A5028061768- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Evolution and Genetic Dynamics
- Antimicrobial Peptides and Activities
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Bacterial Identification and Susceptibility Testing
- Respiratory and Cough-Related Research
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Alzheimer's disease research and treatments
- Amoebic Infections and Treatments
- Piperaceae Chemical and Biological Studies
- Tuberculosis Research and Epidemiology
- Vibrio bacteria research studies
- Gut microbiota and health
- Traditional and Medicinal Uses of Annonaceae
- Enterobacteriaceae and Cronobacter Research
- Asthma and respiratory diseases
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Helicobacter pylori-related gastroenterology studies
- Inhalation and Respiratory Drug Delivery
- Adenosine and Purinergic Signaling
Centro Nacional de Biotecnología
2023-2024
Agrobiotechnology Institute
2016-2024
Gobierno de Navarra
2021-2024
Consejo Superior de Investigaciones Científicas
2017-2023
Universidad Publica de Navarra
2016-2018
Abstract Age-related neurodegenerative diseases involving amyloid aggregation remain one of the biggest challenges modern medicine. Alterations in gastrointestinal microbiome play an active role aetiology neurological disorders. Here, we dissect amyloidogenic properties biofilm-associated proteins (BAPs) gut microbiota and their implications for synucleinopathies. We demonstrate that BAPs are naturally assembled as amyloid-like fibrils insoluble fractions isolated from human microbiota. show...
Tracking bacterial evolution during chronic infection provides insights into how host selection pressures shape genomes. The human-restricted opportunistic pathogen nontypeable Haemophilus influenzae (NTHi) infects the lower airways of patients suffering obstructive pulmonary disease (COPD) and contributes to progression. To identify genetic variation associated with adaptation COPD lung, we sequenced genomes 92 isolates collected from sputum 13 over 1 9 years. Individuals were colonized by...
Antimicrobial resistance (AMR) in bacteria is a major public health problem. The main route for AMR acquisition clinically important the horizontal transfer of plasmids carrying genes. allow to survive antibiotics, but they also entail physiological alterations host cell. Multiple studies over last few years have indicated that these can translate into fitness cost when antibiotics are absent. However, due technical limitations, most based on analysing new associations between and generated...
Abstract Major antibiotic groups are losing effectiveness due to the uncontrollable spread of antimicrobial resistance (AMR) genes. Among these, β-lactam genes –encoding β-lactamases– stand as most common mechanism in Enterobacterales their frequent association with mobile genetic elements. In this context, novel approaches that counter AMR urgently needed. Collateral sensitivity (CS) occurs when acquisition one increases susceptibility another and can be exploited eliminate selectively....
The respiratory pathogen nontypeable Haemophilus influenzae (NTHi) is an important cause of acute exacerbation chronic obstructive pulmonary disease (AECOPD) that requires efficient treatments. A previous screening for host genes differentially expressed upon NTHi infection identified sirtuin-1, which encodes a NAD-dependent deacetylase protective against emphysema and activated by resveratrol. This polyphenol concomitantly reduces viability, therefore highlighting its therapeutic potential...
Nutrient iron sequestration is the most significant form of nutritional immunity and causes bacterial pathogens to evolve strategies host scavenging. Cigarette smoking contains particulates altering lung systemic homeostasis, which may enhance colonization in lungs patients suffering chronic obstructive pulmonary disease (COPD) by opportunistic such as nontypeable. NTHi a heme auxotroph, genome multiple acquisition systems whose role infection requires global understanding. In this study, we...
Airway infection by nontypeable Haemophilus influenzae (NTHi) associates to chronic obstructive pulmonary disease (COPD) exacerbation and asthma neutrophilic airway inflammation. Lipids are key inflammatory mediators in these conditions consequently, NTHi may encounter free fatty acids during persistence. However, molecular information on the interplay NTHi-free is limited, we lack evidence importance of such interaction infection. Maintenance outer membrane lipid asymmetry play an essential...
Many bacterial species actively take up and recombine homologous DNA into their genomes, called natural competence, a trait that offers means to identify the genetic basis of naturally occurring phenotypic variation. Here, we describe "transformed recombinant enrichment profiling" (TREP), in which transformation is used generate complex pools recombinants, selection enrich for specific deep sequencing survey variation responsible. We applied TREP investigate architecture intracellular...
Antibacterial treatment with cotrimoxazol (TxS), a combination of trimethoprim and sulfamethoxazole, generates resistance by, among others, acquisition thymidine auxotrophy associated mutations in the thymidylate synthase gene thyA, which can modify biology infection. The opportunistic pathogen nontypeable Haemophilus influenzae (NTHi) is frequently encountered lower airways chronic obstructive pulmonary disease (COPD) patients, acute exacerbation COPD symptoms. Increasing NTHi to TxS limits...
Chronic obstructive pulmonary disease (COPD) is characterized by abnormal inflammation and impaired airway immunity, providing an opportunistic platform for nontypeable Haemophilus influenzae (NTHi) infection. In this context, therapies targeting not only overactive without significant adverse effects, but also infection are of interest. Increasing evidence suggests that polyphenols, plant secondary metabolites with anti-inflammatory antimicrobial properties, may be protective. Here, a...
Genetic variants arising from within-patient evolution shed light on bacterial adaptation during chronic infection. Contingency loci generate high levels of genetic variation in genomes, enabling to the stringent selective pressures exerted by host. A significant gap our understanding phase-variable contingency is extent their contribution natural infections. The human-adapted pathogen nontypeable Haemophilus influenzae (NTHi) causes persistent infections, which contribute underlying disease...
Abstract Conjugative plasmids promote the dissemination and evolution of antimicrobial resistance in bacterial pathogens. However, plasmid acquisition can produce physiological alterations host, leading to potential fitness costs that determine clinical success bacteria-plasmid associations. In this study, we used a transcriptomic approach characterize interactions between globally disseminated carbapenem plasmid, pOXA-48, diverse collection multidrug resistant enterobacteria. Although...
Conjugative plasmids promote the dissemination and evolution of antimicrobial resistance in bacterial pathogens. However, plasmid acquisition can produce physiological alterations host, leading to potential fitness costs that determine clinical success bacteria-plasmid associations. In this study, we use a transcriptomic approach characterize interactions between globally disseminated carbapenem plasmid, pOXA-48, diverse collection multidrug resistant (MDR) enterobacteria. Although pOXA-48...
Abstract Antimicrobial resistance (AMR) in bacteria is a major public health problem. The main route for AMR acquisition clinically important the horizontal transfer of plasmids carrying genes. allow to survive antibiotics, but they also entail physiological alterations host cell. Multiple studies over last years indicate that these can translate into fitness cost when antibiotics are absent. However, due technical limitations, most based on analysing new associations between and generated...
ABSTRACT Standardized clinical procedures for antibiotic administration rely on pathogen identification and susceptibility testing, often performed single-colony bacterial isolates. For respiratory pathogens, this could be questionable, as chronic patients may persistently colonized by multiple clones or lineages from the same species. Indeed, strains of nontypeable Haemophilus influenzae , with different profiles, can co-isolated cystic fibrosis obstructive pulmonary disease sputum...
Antibiotic resistance is a major Public Health challenge worldwide. Mechanisms other than are described as contributors to therapeutic failure. These include heteroresistance and tolerance, which escape the standardized procedures used for antibiotic treatment decision-making they do not involve changes in minimal inhibitory concentration (MIC). Haemophilus influenzae causes chronic respiratory infection associated with exacerbations suffered by obstructive pulmonary disease (COPD) patients....