Abstract This work examines the potential of predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator other Gram-negative bacteria, as external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) naturally Pseudomonas putida and Cupriavidus necator , or recombinant Escherichia coli strains. B. with a mutated PHA depolymerase gene prevent unwanted breakdown...

Collateral sensitivity (CS) is a promising alternative approach to counteract the rising problem of antibiotic resistance (ABR). CS occurs when acquisition one produces increased susceptibility second antibiotic. Recent studies have focused on strategies designed against ABR mediated by chromosomal mutations. However, main drivers in clinically relevant bacteria horizontal transfer genes plasmids. Here, we report first analysis associated with complete plasmids, including important...

Plasmids are extrachromosomal genetic elements commonly found in bacteria. known to fuel bacterial evolution through horizontal gene transfer (HGT), but recent analyses indicate that they can also promote intragenomic adaptations. However, the role of plasmids as catalysts beyond HGT remains poorly explored. In this study, we investigate impact a widespread conjugative plasmid, pOXA-48, on various multidrug-resistant clinical enterobacteria. Combining experimental and within-patient...

Abstract Major antibiotic groups are losing effectiveness due to the uncontrollable spread of antimicrobial resistance (AMR) genes. Among these, β-lactam genes –encoding β-lactamases– stand as most common mechanism in Enterobacterales their frequent association with mobile genetic elements. In this context, novel approaches that counter AMR urgently needed. Collateral sensitivity (CS) occurs when acquisition one increases susceptibility another and can be exploited eliminate selectively....

Abstract This work aimed to evaluate the predatory activity of Bdellovibrio bacteriovorus 109J on clinical isolates Pseudomonas aeruginosa selected from well-characterized collections cystic fibrosis (CF) lung colonization (n = 30) and bloodstream infections (BSI) 48) including strains by genetic lineage (frequent rare sequence types), antibiotic resistance phenotype (susceptible multidrug-resistant isolates), colony (mucoid non-mucoid isolates). The intraspecies predation range (I-PR) was...

Summary Over the past few decades, considerable interest has been shown in developing nano‐ and microcarriers with biocompatible biodegradable materials for medical biotechnological applications. Microencapsulation is a technology capable of enhancing survival rate bacteria, providing stability harsh environments. In present paper, we developed to encapsulate microorganisms within polyhydroxyalkanoate ( PHA )‐based microcapsules MP s), employing modified double emulsion solvent evaporation...

In this study we analyze the growth-phase dependent metabolic states of Bdellovibrio bacteriovorus by constructing a fully compartmented, mass and charge-balanced genome-scale model predatory bacterium (iCH457). Considering differences between life cycle phases driving growth predator, condition-specific models have been generated allowing systematic its capabilities. Using these computational tools, able to analyze, from system level, dynamic metabolism bacteria as progresses. We provide...

Identifying the nutritional requirements and growth conditions of microorganisms is crucial for determining their applicability in industry understanding role clinical ecology. Predatory bacteria such as Bdellovibrio bacteriovorus have emerged promising tools combating infections by human bacterial pathogens due to natural killing features. Bdellovibrio's lifecycle occurs inside prey cells, using cytoplasm a source nutrients energy. However, this supposes challenge when specific uptake...

Bdellovibrio bacteriovorus HD100 is an obligate predator that preys upon a wide variety of Gram negative bacteria. The biphasic growth cycle includes free-swimming attack phase and intraperiplasmic phase, where the replicates its DNA grows using prey as source nutrients, finally dividing into individual cells (Sockett, 2009). Due to obligatory predatory lifestyle, manipulation requires two-member culturing techniques selected microorganisms ( Lambert et al., 2003 ). In this protocol, we...

Abstract Conjugative plasmids promote the dissemination and evolution of antimicrobial resistance in bacterial pathogens. However, plasmid acquisition can produce physiological alterations host, leading to potential fitness costs that determine clinical success bacteria-plasmid associations. In this study, we used a transcriptomic approach characterize interactions between globally disseminated carbapenem plasmid, pOXA-48, diverse collection multidrug resistant enterobacteria. Although...

Conjugative plasmids promote the dissemination and evolution of antimicrobial resistance in bacterial pathogens. However, plasmid acquisition can produce physiological alterations host, leading to potential fitness costs that determine clinical success bacteria-plasmid associations. In this study, we use a transcriptomic approach characterize interactions between globally disseminated carbapenem plasmid, pOXA-48, diverse collection multidrug resistant (MDR) enterobacteria. Although pOXA-48...

ABSTRACT In this study we analyze the growth-phase dependent metabolic states of Bdellovibrio bacteriovorus by constructing a fully compartmented, mass and charge-balanced genome-scale model predatory bacterium ( i CH457). Considering differences between life cycle phases driving growth predator, condition-specific models have been generated allowing systematic its capabilities. Using these unprecedented computational tools, able to analyze, from system level, dynamic metabolism bacteria as...

Abstract Collateral sensitivity (CS) is a promising alternative approach to counteract the rising problem of antibiotic resistance (ABR). CS occurs when acquisition one produces increased susceptibility second antibiotic. For be widely applicable in clinical practice, it would need effective against different mechanisms available bacteria. Recent studies have focused on strategies designed ABR mediated by chromosomal mutations. However, main drivers clinically relevant bacteria horizontal...

ABSTRACT The use of predatory bacteria as live antibiotics has been proposed for managing bacterial infections, especially those caused by antibiotic multiresistant isolates which there are few therapeutic options. However, the current knowledge in this field is scarce, with most available data based on environmental isolates, a significant lack human clinical samples. In study, we evaluated spectrum reference strain Bdellovibrio bacteriovorus 109J 13 Serratia marcescens (5 were...

Abstract Major antibiotic groups are losing effectiveness due to the uncontrollable spread of antimicrobial resistance (AMR) genes. Among these, β-lactam genes –encoding β-lactamases– stand as most common mechanism in Enterobacterales their frequent association with mobile genetic elements. In this context, novel approaches that counter AMR urgently needed. Collateral sensitivity (CS) occurs when acquisition one increases susceptibility another and can be exploited selectively eliminate AMR....

ABSTRACT Identifying the nutritional requirements and growth conditions of microorganisms is crucial for determining their applicability in industry understanding role clinical ecology. Predatory bacteria such as Bdellovibrio bacteriovorus have emerged promising tools combating infections by human bacterial pathogens due to natural killing features. Bdellovibrio’s lifecycle occurs inside prey cells, using cytoplasm a source nutrients energy. However, this supposes challenge when specific...