A5039788965- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Antimicrobial agents and applications
- Electrospun Nanofibers in Biomedical Applications
- Biofuel production and bioconversion
- Advanced Cellulose Research Studies
- Microbial Fuel Cells and Bioremediation
- Biopolymer Synthesis and Applications
- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Gene Regulatory Network Analysis
- Graphene and Nanomaterials Applications
- Carbon dioxide utilization in catalysis
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Blood transfusion and management
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Recycling and Waste Management Techniques
- Microbial bioremediation and biosurfactants
- Photosynthetic Processes and Mechanisms
Consejo Superior de Investigaciones Científicas
2015-2025
Centro de Investigaciones Biológicas Margarita Salas
2015-2024
Centro Nacional de Biotecnología
2018-2021
Hospital Universitario Ramón y Cajal
2020
Waterford Institute of Technology
2014
Hospital Universitario de La Princesa
2010-2013
Unidades Centrales Científico-Técnicas
1996-1999
ETH Zurich
1999
Comunidad de Madrid
1994
Two hundred and seventy five million tons of plastic waste were produced in 2010 alone (Jambeck et al., 2015), with Europe accounting for about 55 per year. The environmental impact these, primarily fossil-based, plastics has been broadly discussed. While the vast majority these polymers are not biodegradable, their strength light weight provide comparative advantages. Poly(ethylene terephthalate) (PET), instance, contributed significantly to reducing energy expenditure during transport,...
Worldwide plastic production has surged over the past 50 years. In 2016, it reached 335 million tonnes per annum, with Europe alone producing 60 tonnes. Over next 20 years, is expected to double. Plastic packaging most important product (26% of total volume all plastics used), although a short life compared used in, for example, construction and car industries. producers transformers are keen highlight benefits derived from packaging; not only does deliver direct economic profits, but also...
Summary The synthesis and degradation of polyhydroxyalkanoates (PHAs), the storage polymer many bacteria, is linked to operation central carbon metabolism. To rationalize impact PHA accumulation on metabolism prototype bacterium Pseudomonas putida , we have revisited production in quantitative physiology experiments wild‐type strain vs. a negative mutant growing under low nitrogen conditions. When octanoic acid was used as precursor energy source, detected higher intracellular flux via...
Modular cloning has become a benchmark technology in synthetic biology. However, notable disparity exists between its remarkable development and the need for standardization to facilitate seamless interoperability among systems. The field is thus impeded by an overwhelming proliferation of organism-specific systems that frequently lack compatibility. To overcome these issues, we present Golden Standard (GS), Type IIS assembly method underpinned European Vector Architecture. GS unlocks...
Abstract Bioplastics, comprised of bio‐based and/or biodegradable polymers, have the potential to play a crucial role in transition towards sustainable circular economy. The use polymers not only leads reduced greenhouse gas emissions but also might address problem plastic waste persisting environment, especially when removal is challenging. Nevertheless, plastics should be considered as substitutes for proper management practices, given that their biodegradability strongly depends on...
Bacterial polyhydroxyalkanoates (PHAs) have emerged as promising eco-friendly alternatives to petroleum-based plastics since they are synthesized from renewable resources and offer exceptional properties. However, their production is limited the stationary growth phase under nutrient-limited conditions, requiring customized strategies costly two-phase bioprocesses. In this study, we tackle these challenges by employing a model-driven approach reroute carbon flux remove regulatory constraints...
Escherichia coli W uses the aromatic compound 4-hydroxyphenylacetate (4-HPA) as a sole source of carbon and energy for growth. The monooxygenase which converts 4-HPA into 3,4-dihydroxyphenylacetate, first intermediate pathway, consists two components, HpaB (58.7 kDa) HpaC (18.6 kDa), encoded by hpaB hpaC genes, respectively, that form single transcription unit. Overproduction small component in E. K-12 cells has facilitated purification protein, was revealed to be homodimer catalyzes...
ABSTRACT The phaC1 gene codes for the medium-chain-length polyhydroxyalkanoate (mcl PHA) synthase of Pseudomonas oleovorans GPo1, which produces mcl PHA when grown in an excess carbon source and under nitrogen limitation. In this work, we have demonstrated, by constructing a recombinant P. strain carrying :: lacZ reporter system, that is expressed efficiently presence octanoic acid while its expression repressed glucose or citrate used as source. Moreover, GPo1 mutant (strain GPG-Tc6)...
We have determined and analyzed the nucleic acid sequence of a 14,855-bp region that contains complete gene cluster encoding 4-hydroxyphenylacetic (4-HPA) degradative pathway Escherichia coli W (ATCC 11105). This catabolic is composed by 11 genes, i.e., 8 enzyme-encoding genes distributed in two putative operons, hpaBC (4-HPA hydroxylase operon) hpaGEDFHI (meta-cleavage operon); 2 regulatory hpaR hpaA; gene, hpaX, encodes protein related to superfamily transmembrane facilitators appears be...
The nucleotide sequences of the hpaB and hpaC genes encoding 4-hydroxyphenylacetate 3-hydroxylase from Escherichia coli W ATCC 11105 have been determined.These appear to be part an operon encode two proteins 58,781 18,679 Da, respectively, that are required for hydroxylase activity.This aromatic is NADH-dependent uses FAD as redox chromophore.The largest component (HpaB) has purified by affinity chromatography in Cibacron blue.E. cells express exclusively showed only a very low activity was...
Summary The PhaF is a nucleoid‐associated like protein of Pseudomonas putida KT2442 involved in the polyhydroxyalkanoate (PHA) metabolism. Its primary structure shows two modular domains; N‐terminal PHA granule‐binding domain (phasin domain) and C‐terminal half containing AAKP‐like tandem repeats characteristic histone H1 family. Although binding to granules its role as transcriptional regulator have been previously demonstrated, cell physiology meaning these properties remains unknown. This...
Abstract This work examines the potential of predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator other Gram-negative bacteria, as external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) naturally Pseudomonas putida and Cupriavidus necator , or recombinant Escherichia coli strains. B. with a mutated PHA depolymerase gene prevent unwanted breakdown...
Shoe patterns and a sole made of BC, baby shoe BC and, on the right material dyed in red. (prototype by www.patent-shoes.com). All consumer goods – including fashion products use up resources; there is no exception to this rule. According Mark Sumner University Leeds (UK), world has some major sustainability problems. By 2030, it predicted that industry's water consumption will grow 50 per cent 118 billion cubic metres, its carbon footprint increase 2,791 tonnes, amount waste creates hit 148...
Abstract Organic wastes are a suitable feedstock for the production of value‐added products that have been insufficiently exploited due to their complexity, which challenges transformation by conventional procedures. Gasification and pyrolysis organic can reduce this complexity producing syngas ( CO plus H 2 other C1 gases), be used as valuable commodity catalytic conversion into chemicals. However, high cost susceptibility poisoning chemical catalysts encouraged research on biocatalysts...
Abstract This article introduces the EU Horizon 2020 research project MIX-UP, "Mixed plastics biodegradation and upcycling using microbial communities". The focuses on changing traditional linear value chain of to a sustainable, biodegradable based one. Plastic mixtures contain five top six fossil-based recalcitrant [polyethylene (PE), polyurethane (PUR), polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS)], along with upcoming bioplastics polyhydroxyalkanoate (PHA)...
Innovative approaches for recycling and upcycling of textile waste are required to sustainably treat the tremendous, discarded amounts. Due their high specificity mild reaction conditions, enzymes offer an environmentally friendly alternative separation blended materials. Glucose was recovered from cellulose (viscose) polyamide blends utilized as carbon source biosynthesis two biodegradable polymers. Polyhydroxybutyrate (PHB) produced by Cupriavidus necator reaching intracellular...
Novel biodegradable bacterial plastics, made up of units 3-hydroxy-n-phenylalkanoic acids, are accumulated intracellularly by Pseudomonas putida U due to the existence in this bacterium (i) an acyl-CoA synthetase (encoded fadD gene) that activates aryl-precursors; (ii) a β-oxidation pathway affords 3-OH-aryl-CoAs, and (iii) polymerization-depolymerization system phalocus) integrated two polymerases (PhaC1 PhaC2) depolymerase (PhaZ). The complete assimilation these compounds requires...