A5052759613- Animal Ecology and Behavior Studies
- Pharmacogenetics and Drug Metabolism
- Vitamin K Research Studies
- Yersinia bacterium, plague, ectoparasites research
- Leptospirosis research and findings
- Poisoning and overdose treatments
- Vitamin C and Antioxidants Research
- Hormonal Regulation and Hypertension
- Insect and Pesticide Research
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Pesticide Exposure and Toxicity
- Carbohydrate Chemistry and Synthesis
- Drug Transport and Resistance Mechanisms
- Plant chemical constituents analysis
- Viral Infections and Vectors
- Zoonotic diseases and public health
- Pesticide Residue Analysis and Safety
- Effects and risks of endocrine disrupting chemicals
- Phytochemical compounds biological activities
- Neuroendocrine regulation and behavior
- Analytical Chemistry and Chromatography
- Connective tissue disorders research
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
VetAgro Sup
2016-2025
Université Claude Bernard Lyon 1
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2012-2024
Gouvernance, Risque, Environnement, Développement
2015-2022
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
2016
Institut National des Sciences Appliquées de Lyon
2016
Laboratoire de Chimie Organique
2016
Centre National de la Recherche Scientifique
2004-2016
Institut National de la Recherche Agronomique
2002-2015
Ecologie Microbienne Lyon
2014
When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or monomeric apoprotein solution. Analytical and spectroscopic studies of wild-type protein site-directed variants structural characterization the by using x-ray crystallography indicate that contains subunit-bridging [2Fe-2S] cluster is ligated catalytic cysteines two glutaredoxins glutathiones. Mutagenesis data on variety suggest incorporation an...
Second-generation anticoagulant rodenticides (SGARs) are persistent chiral pesticides used to control rodent populations. Raptors protected species and may be exposed through the ingestion of rodents contaminated with SGARs. Commercial formulations SGARs a mixture four stereoisomers (E1, E2, E3, E4): cis- trans-diastereoisomers each racemic two enantiomers. In this study, residue levels all (bromadiolone, difenacoum, brodifacoum, difethialone, flocoumafen) were evaluated in liver 529 raptor...
Vitamin K is involved in the γ-carboxylation of vitamin K-dependent proteins, and epoxide a by-product this reaction. Due to limited intake K, its regeneration necessary involves 2,3-epoxide reductase (VKOR) activity. This activity known be supported by VKORC1 protein, but recently second gene, VKORC1L1, appears able support when encoded protein expressed HEK293T cells. Nevertheless, was described as being responsible for driving K-mediated antioxidation pathways. In paper we precisely...
Brown rats are one of the most widespread urban species worldwide. Despite nuisances they induce and their potential role as a zoonotic reservoir, knowledge on rat populations remains scarce. The main purpose this study was to characterize an brown population from Chanteraines park (Hauts-de-Seine, France), with regards haematology, genetics, immunogenic diversity, resistance anticoagulant rodenticides, community parasites. Haematological parameters were measured. Population genetics...
Leptospirosis, a bacterial zoonosis with worldwide distribution, represents major public health challenge. It is caused by the spirochete Leptospira, whose main reservoir in urban environments brown rat ( Rattus norvegicus ). Understanding transmission dynamics of this disease within population essential for controlling risk human infection. In study, an original capture method was used to analyze variations carriage and load according age two distinct populations rats, provide better...
We determined whether the two major structural modifications, i.e. phosphorylation and sulfation of glycosaminoglycan-protein linkage region (GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1), govern specificity glycosyltransferases responsible for biosynthesis tetrasaccharide primer. analyzed influence C-2 Xyl residue on human beta1,4-galactosyltransferase 7 (GalT-I), which catalyzes transfer Gal onto Xyl, we evaluated consequences C-4/C-6 Galbeta1-3Gal (Gal2-Gal1) activity...
Second-generation anticoagulant rodenticides (SGARs) have been used since the 1980s for pest management. They are highly efficient even in warfarin-resistant rodents. Nevertheless, because of their tissue persistence, nontarget poisoning by SGARs is commonly described wildlife. Due to this major problem, a new generation anticoagulants must be developed limit risk. This study proposes method developing revisiting old based on concept stereochemistry. Each current SGAR mixture diastereomers....
Abstract Anticoagulant rodenticides are commonly used to control rodent pests worldwide. They specifically inhibit the vitamin K epoxide reductase ( VKORC 1), which is an enzyme encoded by Vkorc1 gene, involved in recycling of K. Therefore, they prevent blood clotting. Numerous mutations gene were reported rodents, and some resistant phenotype. Two hundred sixty‐six mice tails received from 65 different locations France. Coding sequences sequenced order detect mutations. Consequences...
To characterize the contribution of human flavin-containing monooxygenase form 3 (FMO3) in metabolism and disposition drugs xenobiotics, we determined single nucleotide polymorphisms coding region adjacent splice junctions <i>FMO3</i> 134 African Americans 120 Caucasians from United States. In regions examined, DNA resequencing or high throughput MassEXTEND studies coupled with mass spectrometric genotyping showed that 12 sites variation were present. Three variants encoding synonymous...
Difenacoum, an antivitamin K anticoagulant, has been widely used as rodenticide to manage populations of rodents. Difenacoum belongs the second generation and, all molecules belonging difenacoum is often involved in primary poisonings domestic animals and secondary wildlife by feeding contaminated To develop a new ecofriendly difenacoum, we explored this study differences properties between diastereomers difenacoum. Indeed, currently commercial mixture 57% cis-isomers 43% trans-isomers. Cis-...
Rodents are the primary reservoirs for pathogenic Leptospira species, which cause leptospirosis. Among key potential carriers water voles, whose population outbreaks can consequently pose a major threat to human and animal health. We studied prevalence, prominence, epidemiology of species in voles central France. First, 46 were captured, DNA was extracted from kidney, lung, liver, blood, urine tested presence using three molecular methods: PCR, O-antigen typing, variable number tandem repeat...
Vitamin K family molecules—phylloquinone (K1), menaquinone (K2), and menadione (K3)—act as γ-glutamyl carboxylase (GGCX)-exclusive cofactors in their hydroquinone state, activating proteins of main importance for blood coagulation the liver arterial calcification prevention energy metabolism extrahepatic tissues. Once GGCX is activated, vitamin found epoxide which then recycled to quinone states by reductase (VKORC1). Nevertheless, little information available concerning K1, K2, or K3 tissue...
The systematic use of antivitamin K anticoagulants (AVK) as rodenticides caused the selection rats resistant to AVKs. resistance is mainly associated genetic polymorphisms in Vkorc1 gene encoding VKORC1 enzyme responsible for reduction vitamin 2,3-epoxide K. Five major mutations, which are AVK resistance, have been described. Possible explanations biological cost these mutations suggested. This might be linked an increase requirements. To analyze possible involvement this cost, rVKORC1 and...
In spite of intensive use bromadiolone, rodent control was inefficient on a farm infested by rats in Zaragoza, Spain. While metabolic resistance previously described this species, the observation target to antivitamin K rodenticides had been poorly documented Rattus rattus.From trapped farm, cytochrome b and Vkorc1 genes were amplified PCR sequenced order identify species detect potential mutations. VKORC1-deduced amino acid sequences thus expressed Pichia pastoris, inhibition constants...
Abstract Leptospirosis is a re-emergent worldwide zoonosis. It endemic in Martinique where transmission conditions are favourable. Humans usually infected through contact with water contaminated urine of rodents. Recent human leptospirosis outbreaks require today effective rodent management to prevent transmission. Nowadays, use anticoagulant rodenticides (AR) the main method implemented control populations. Nevertheless, intensive these AR has selected many VKORC1-based resistant strains...