A5034443977- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Estrogen and related hormone effects
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Advanced Glycation End Products research
- S100 Proteins and Annexins
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- HER2/EGFR in Cancer Research
- Metabolism, Diabetes, and Cancer
- Fibroblast Growth Factor Research
- Cancer-related molecular mechanisms research
- Biomarkers in Disease Mechanisms
- Epigenetics and DNA Methylation
- Crystallography and molecular interactions
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Advanced Breast Cancer Therapies
- Immune cells in cancer
- Wnt/β-catenin signaling in development and cancer
- Neonatal Respiratory Health Research
University of Calabria
2018-2024
Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione
2018
Renfe Operadora (Spain)
2018
Focal adhesion kinase (FAK) is a cytoplasmatic protein tyrosine that associates with both integrins and growth factor receptors toward the adhesion, migration invasion of cancer cells. The G-protein coupled estrogen receptor (GPER) has been involved in stimulatory action estrogens breast tumor. In this study, we have investigated engagement FAK by GPER signaling triple negative (TNBC) cells.Publicly available large-scale database patient data sets derived from "The Cancer Genome Atlas"...
Triple-negative breast cancer (TNBC) is an aggressive tumor subtype that currently lacks targeted treatment options. The role played by the insulin-like growth factor-1 (IGF-1) and its cognate receptor IGF-1R in TNBC has been reported. Nevertheless, molecular mechanisms which IGF-1/IGF-1R system may contribute to progression still remains be fully understood. By computational analysis of vast genomics information public databases (TCGA METABRIC), we obtained evidence high IGF-1 or levels...
We report a novel highly crystalline MOF, featuring hydroxyl-decorated channels, capable of distinctly organizing guest organic molecules within its pores.
Abstract Background Hypoxia plays a relevant role in tumor-related inflammation toward the metastatic spread and cancer aggressiveness. The pro-inflammatory cytokine interleukin-1β (IL-β) its cognate receptor IL1R1 contribute to initiation progression of breast determining pro-tumorigenic inflammatory responses. transcriptional target hypoxia inducible factor-1α (HIF-1α) namely G protein estrogen (GPER) mediates feedforward loop coupling IL-1β induction by cancer-associated fibroblasts...
The FGF2/FGFR1 paracrine loop is involved in the cross-talk between breast cancer cells and components of tumor stroma as cancer-associated fibroblasts (CAFs). By quantitative PCR (qPCR), western blot, immunofluorescence analysis, ELISA ChIP assays, we demonstrated that 17β-estradiol (E2) G protein estrogen receptor (GPER) agonist G-1 induce up-regulation secretion FGF2 via GPER together with EGFR/ERK/c-fos/AP-1 signaling cascade (ER)-negative primary CAFs. Evaluating genetic alterations...
The G protein-coupled estrogen receptor (GPER, formerly known as GPR30) is a seven-transmembrane that mediates signals in both normal and malignant cells. In particular, GPER has been involved the activation of diverse signaling pathways toward transcriptional biological responses characterize progression breast cancer (BC). this context, correlation between expression worse clinical-pathological features BC suggested, although controversial data have also reported. order to better assess...
Breast cancer represents the most frequently diagnosed malignancy in women worldwide. Various therapeutics are currently used order to halt progression of breast tumor, even though certain side effects may limit beneficial effects. In recent years, many efforts have been addressed usefulness natural compounds as anticancer agents due their low toxicity. Resveratrol, a stilbene found grapes, berries, peanuts and soybeans, has raised notable interest for its antioxidant, anti-inflammatory,...
Background: Breast cancer (BC) mortality is increased among obese and diabetic patients. Both obesity diabetes are associated with dysregulation of both the IGF-1R RAGE (Receptor for Advanced Glycation End Products) pathways, which contribute to complications these disorders. The alarmin S100A7, signaling through receptor RAGE, prompts angiogenesis, inflammation, BC progression. Methods: We performed bioinformatic analysis gene expression datasets from published studies. then used Estrogen...
Abstract Background Metabolic disorders are associated with increased incidence, aggressive phenotype and poor outcome of breast cancer (BC) patients. For instance, hyperinsulinemia is an independent risk factor for BC the insulin/insulin receptor (IR) axis involved in growth metastasis. Of note, anti-diabetic metformin may be considered comprehensive therapeutic approaches on basis its antiproliferative effects obtained diverse pre-clinical clinical studies. Methods Bioinformatics analysis...
The receptor for advanced glycation-end products (RAGE) and its ligands have been implicated in obesity associated inflammatory processes as well metabolic alterations like diabetes. In addition, RAGE-mediated signaling has reported to contribute the metastatic progression of breast cancer (BC), although mechanistic insights are still required. Here, we provide novel findings regarding transcriptomic landscape molecular events through which RAGE may prompt aggressive features estrogen...
Abstract Background Estetrol (E4) is a natural estrogen produced by the fetal liver during pregnancy. Due to its favorable safety profile, E4 was recently approved as estrogenic component of new combined oral contraceptive. selective ligand receptor (ER)α and ERβ, but binding G Protein-Coupled Estrogen Receptor (GPER) has not been described date. Therefore, we aimed explore action in GPER-positive Triple-Negative Breast Cancer (TNBC) cells. Methods The potential interaction between GPER...
Abstract Background Over the last two decades, tumor-derived RNA expression signatures have been developed for most commonly diagnosed tumors worldwide, namely prostate and breast tumors, in order to improve both outcome prediction treatment decision-making. In this context, molecular gained by main components of tumor microenvironment, such as cancer-associated fibroblasts (CAFs), explored prognostic therapeutic tools. Nevertheless, a deeper understanding significance CAFs-related gene...
Estrogens acting through the classic estrogen receptors (ERs) and G protein receptor (GPER) regulate expression of diverse miRNAs, small sequences non-coding RNA involved in several pathophysiological conditions, including breast cancer. In order to provide novel insights on miRNAs regulation by estrogens tumor, we evaluated 754 TaqMan Array ER-negative GPER-positive SkBr3 cancer cells cancer-associated fibroblasts (CAFs) upon 17β-estradiol (E2) treatment. Various were regulated E2 a...
Abstract The G protein-coupled estrogen receptor (GPER) mediates action in different pathophysiological conditions, including cancer. GPER expression and signaling have been found to join the progression of triple-negative breast cancer (TNBC), even though controversial data reported. In present study, we aimed at providing new mechanistic biological discoveries knocking out (KO) by CRISPR/Cas9 technology MDA-MB-231 TNBC cells. KO whole transcriptome respect wild type (WT) cells was...
Advanced glycation end products (AGEs) and the cognate receptor, named RAGE, are involved in metabolic disorders characterized by hyperglycemia, type 2 diabetes mellitus (T2DM) obesity. Moreover, AGEs/RAGE transduction pathway prompts a dysfunctional interaction between breast cancer cells tumor stroma toward acquisition of malignant features. However, action axis main players microenvironment, cancer-associated fibroblasts (CAFs), remains to be fully explored. In present study, chemokine...
Abstract The synthetic peptide ERα17p (sequence: PLMIKRSKKNSLALSLT), which corresponds to the 295–311 region of human estrogen receptor α (ERα), induces apoptosis in breast cancer cells. In mice and at low doses, it promotes not only decrease size xenografted triple-negative tumors, but also anti-inflammatory anti-nociceptive effects. Recently, we have shown that these effects were due its interaction with seven-transmembrane G protein-coupled GPER. Following modeling studies, C-terminus...
Triple-negative breast cancer (TNBC) is an aggressive tumor subtype characterized by poor clinical outcome. In recent years, numerous advancements have been made to better understand the biological landscape of TNBC, though appropriate targets still remain be determined. present study, we determined that expression levels FGF2 and S100A4 are higher in TNBC with respect non-TNBC patients when analyzing “The Invasive Breast Cancer Cohort The Genome Atlas” (TCGA) dataset. addition, found gene...
The cyclin D1-cyclin dependent kinases (CDK)4/6 inhibitor palbociclib in combination with endocrine therapy shows remarkable efficacy the management of estrogen receptor (ER)-positive and HER2-negative advanced breast cancer (BC). Nevertheless, resistance to frequently arises, highlighting need identify new targets toward more comprehensive therapeutic strategies BC patients. cell lines resistant were generated used as a model system. Gene silencing techniques overexpression experiments,...
Abstract Onobrychis carduchorum (Fabaceae) is a plant widely employed in Kurdish traditional medicine to cure wounds, inflammations, and other skin diseases. We could isolate ten different polyphenols from the acetone extract of this plant: 1–4 are isoflavones, having genistein skeleton; 5–7 flavanones, naringenin 8–10 prenylated dihydro-stilbenes. In particular, have been isolated, so far, only Glycyrrhiza glabra (liquorice). Many above phenols showed significant toxicity on some human...
Metabolic disorders, such as obesity, type 2 diabetes (T2D) and metabolic syndrome, have been implicated in breast cancer (BC) progression. In this regard, insulin has shown to promote mitogenic metastatic responses BC through diverse signaling pathways. Moreover, high levels of elevated expression its cognate receptor, namely receptor (IR), associated with increased incidence, resistance treatments poor outcomes. Metformin (1,1-dimethylbiguanide hydrochloride) is the most commonly...