A5041595457- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Bioenergy crop production and management
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Gold and Silver Nanoparticles Synthesis and Applications
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Plasmonic and Surface Plasmon Research
- Antibiotic Resistance in Bacteria
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Evolution and Genetic Dynamics
- Plant tissue culture and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Escherichia coli research studies
- Molecular Sensors and Ion Detection
- Bacterial Genetics and Biotechnology
- Chemical Synthesis and Analysis
- Barrier Structure and Function Studies
HUN-REN Szegedi Biológiai Kutatóközpont
2016-2024
Institute of Plant Biology
2016-2024
Hungarian Research Network
2021-2023
Institute of Biochemistry
2021
University of Szeged
2003-2020
Hungarian Academy of Sciences
2015-2019
Semmelweis University
1976
Antibiotic development is frequently plagued by the rapid emergence of drug resistance. However, assessing risk resistance in preclinical stage difficult. Standard laboratory evolution approaches explore only a small fraction sequence space and fail to identify exceedingly rare mutations combinations thereof. Therefore, new exhaustive methods are needed accurately assess potential uncover underlying mutational mechanisms. Here, we introduce directed with random genomic (DIvERGE), method that...
The biomass productivity of the energy willow Salix viminalis as a short-rotation woody crop depends on organ structure and functions that are under control genome size. Colchicine treatment axillary buds resulted in set autotetraploid S. var. Energo genotypes (polyploid [PP-E]; 2n = 4x 76) with variation green pixel-based shoot surface area. In cases where increased was observed, it primarily derived from larger leaf size wider stem diameter. Autotetraploidy slowed primary growth diameter...
Multitargeting antibiotics, i.e., single compounds capable of inhibiting two or more bacterial targets, are generally considered to be a promising therapeutic strategy against resistance evolution. The rationale for this theory is that multitargeting antibiotics demand the simultaneous acquisition multiple mutations at their respective target genes achieve significant resistance. presumes individual provide little no benefit host.
Inefficient drug delivery across the blood–brain barrier (BBB) and into target cells in brain hinders treatment of neurological diseases. One strategy to increase penetration drugs is use vesicular nanoparticles functionalized with multiple ligands BBB transporters as vehicles. Once within brain, however, must also be able reach their therapeutic targets different cell types. It is, therefore, favorable if such nanocarriers are designed that can deliver cargo not only endothelial cells, but...
Abstract In vitro regeneration of Arabidopsis from roots is generally achieved via indirect organogenesis. First, transdifferentiation lateral root primordia to calli by a balanced auxin-to-cytokinin ratio that followed the induction shoot meristem formation using high cytokinin level. Here we demonstrate if explants were transferred onto hormone-free medium after transient (4-days) treatment, embryogenic marker genes ( LEC1, LEC2, FUS3 ) started be expressed. App. 50% foci developed into...
Nanosized drug delivery systems targeting transporters of the blood-brain barrier (BBB) are promising carriers to enhance penetration therapeutics into brain. The expression solute (SLC) is high and shows a specific pattern at BBB. Here we show that ligands ascorbic acid, leucine glutathione on nanoparticles elevated uptake albumin cargo in cultured primary rat brain endothelial cells. Moreover, demonstrated ability triple-targeted nanovesicles deliver their midbrain organoids after crossing...
Abstract A simple sheathless capillary electrophoresis (CE)/mass spectrometry (MS) interface was constructed by combining widely used nanospray needles with fused‐silica capillaries and it successfully applied for the separation of peptides. The end CE pulled to a taper, etched then fitted into metal‐coated borosilicate capillary. needle can be several runs, but easily rapidly changed in case accidental breakage or evaporation coating. fast electrochromatographic method also developed MS...
Presently, targeted gene mutagenesis attracts increasing attention both in plant research and crop improvement. In these approaches, successes are largely dependent on the efficiency of delivery editing components into cells. Here, we report optimization cationic polymer poly(2-hydroxypropylene imine) (PHPI)-mediated plasmid DNAs, or single-stranded oligonucleotides labelled with Cyanine3 (Cy3) 6-Carboxyfluorescein (6-FAM)-fluorescent dyes maize protoplasts. Co-delivery GFP-expressing...
Plasmonically enhanced fluorescence is a widely studied and applied phenomenon, however, only comparative theoretical experimental analysis of coupled fluorophores plasmonic nanoresonators makes it possible to uncover how this phenomenon can be controlled. A numerical optimization method was design configurations that are capable resulting in an enhancement excitation emission, moreover, both phenomena simultaneously Cy5 dye molecule gold nanorod systems. Parametric sensitivity studies...
Hybrid vigor and polyploidy are genetic events widely utilized to increase the productivity of crops. Given that bioenergy usage needs be expanded, we investigated triploid hybrid in terms biology biomass-related willow traits their relevance control biomethane production. To produce genotypes, crossed two female diploid Swedish cultivars (Inger, Tordis) with male autotetraploid (Salix viminalis) variants (PP-E7, PP-E15). Field studies at locations successive years recorded considerable...
Microfluidics-based protoplast isolation, which enabled intracellular uptake of singlet oxygen sensor fluorophore and DNA oligonucleotides in Symbiodinium spp.
Successful use of woody species in reducing climatic and environmental risks energy shortage spreading pollution requires deeper understanding the physiological functions controlling biomass productivity phytoremediation efficiency. Targets breeding willow include size functionality root system. For combination polyploidy heterosis, we have generated triploid hybrids (THs) by crossing autotetraploid plants with leading cultivars (Tordis Inger). These novel Salix genotypes (TH3/12, TH17/17,...
The synthesis, chemical, physical, biological, spectroscopic and miscellaneous analytical properties of Nalkylguanine derivatives substituted at 1-, 1,N²-, N -, 3-, 3,N 7-, 7,9- 9-positions have been surveyed, mainly from the 2003-2009 period. Beyond synthetic methods, particular emphasis has given to products mutagenesis carcinogenesis, role modified fluorescent guanosines (wyosine, wyebutosine), mRNA cap structures drugs stemming N-alkylguanines. review is based on 154 references contains...
Cysteinyl nucleoside and homonucleoside oligomers, the first representatives of a new class artificial nucleic acid family were prepared.
Abstract ssDNA recombineering has been exploited to hyperdiversify genomically-encoded nanobodies displayed on the surface of Escherichia coli for originating new binding properties. As a proof-of-principle nanobody recognizing antigen TirM from enterohaemorrhagic E. (EHEC) was evolved towards otherwise not recognized enteropathogenic (EPEC). To this end, cells displaying fused intimin outer membrane-bound domain were subjected multiple rounds mutagenic oligonucleotide targeting...
The family of cisplatin and its analogues contains some the most powerful anticancer drugs developed to date and, since their discovery, extensive efforts have been devoted understand mode action. 9-Alkylguanines featured widely in these studies as model nucleobases. This review describes both synthesis chemical, biological miscellaneous spectroscopic properties a variety transition metal ion (Pt, Pd, Ru, Os, Rh, Re, Tc, Zn, Cu, Cd) complexes N-alkylguanines (mostly 9- alkylguanines) which...
In macromolecular crystallography, a great deal of effort has been invested in understanding radiation-damage progression. While the sensitivity protein crystals well characterized, DNA and DNA–protein complexes have not thus far studied as thoroughly. Here, systematic investigation radiation damage to crystal 16-mer diffracting 1.8 Å resolution held at 100 K, up an absorbed dose 45 MGy, is reported. The RIDL ( Radiation-Induced Density Loss ) automated computational tool was used for...
Plant Rho-type GTPases (ROPs) are versatile molecular switches involved in a number of signal transduction pathways. Although it is well known that they indirectly linked to protein kinases, our knowledge about their direct functional interaction with upstream or downstream kinases scarce. It reasonable suppose similarly animal counterparts, ROPs might also be regulated by phosphorylation. There only, however, very limited experimental evidence support this view. Here, we present the...
Multi-targeting antibiotics, i.e. single compounds capable to inhibit two or more bacterial targets offer a promising therapeutic strategy, but information on resistance evolution against such drugs is scarce. Gepotidacin an antibiotic candidate that selectively inhibits both DNA gyrase and topoisomerase IV. In susceptible organism, Klebsiella pneumoniae , combination of specific mutations in these target proteins provide over 2000-fold increment resistance, while individually none affect...