A5048858422- Computational Drug Discovery Methods
- Contact Dermatitis and Allergies
- Allergic Rhinitis and Sensitization
- Nerve injury and regeneration
- Biomedical Text Mining and Ontologies
- Chemical Safety and Risk Management
- Signaling Pathways in Disease
- Exercise and Physiological Responses
- Thermoregulation and physiological responses
- Transgenic Plants and Applications
- Evaluation and Performance Assessment
- Muscle metabolism and nutrition
- Immune Cell Function and Interaction
- Hereditary Neurological Disorders
- Genetics, Bioinformatics, and Biomedical Research
- Clinical Nutrition and Gastroenterology
- Stress Responses and Cortisol
- Dermatology and Skin Diseases
- Biomedical and Engineering Education
- Risk and Safety Analysis
- Effects and risks of endocrine disrupting chemicals
- Food Allergy and Anaphylaxis Research
- Cancer, Stress, Anesthesia, and Immune Response
- T-cell and B-cell Immunology
- Food Safety and Hygiene
University of Applied Sciences Utrecht
2014-2025
Utrecht University
2002-2024
Maastricht University
2024
University Medical Center Utrecht
2002-2004
Wilhelmina Children's Hospital
2003
Netherlands Institute for Neuroscience
1997
Both because of the shortcomings existing risk assessment methodologies, as well newly available tools to predict hazard and with machine learning approaches, there has been an emerging emphasis on probabilistic assessment. Increasingly sophisticated AI models can be applied a plethora exposure data obtain not only predictions for particular endpoints but also estimate uncertainty outcome. This provides basis shift from deterministic more approaches comes at cost increased complexity process...
The first Stakeholder Network Meeting of the EU Horizon 2020-funded ONTOX project was held on 13–14 March 2023, in Brussels, Belgium. discussion centred around identifying specific challenges, barriers and drivers relation to implementation non-animal new approach methodologies (NAMs) probabilistic risk assessment (PRA), order help address issues rank them according their associated level difficulty. aims advance chemical humans, without use animal testing, by developing NAMs PRA line with...
Chemical safety assessment still heavily relies on animal testing, presenting ethical dilemmas and limited human predictive value. New approach methodologies (NAMs), including in vitro silico techniques, offer alternative solutions. In toxicology has made progress predicting chemical effects but frequently lacks biological mechanistic foundations. Recent developments focus understanding of adverse inflicted by chemicals, as embedded (quantitative) outcome pathways (AOPs). However, there is a...
Outgrowth of solid tumors requires blood supply to the tumor. Tumor angiogenesis is dependent on interplay between tumor-derived angiogenic factors and stromal cells. Recently, it has been shown that neurotransmitter dopamine a potent inhibitor VEGF-induced angiogenesis. Moreover, there evidence patients with schizophrenia have hyperreactive dopaminergic system are relatively protected from cancer. We hypothesized hyperreactivity related reduced tumor development. Therefore, we investigated...
Adverse outcome pathway (AOP) networks are versatile tools in toxicology and risk assessment that capture visualize mechanisms driving toxicity originating from various data sources. They share a common structure consisting of set molecular initiating events key events, connected by event relationships, leading to the actual adverse outcome. AOP be considered living documents should frequently updated feeding new data. Such iterative optimization exercises typically done manually, which not...
This assay does not distinguish a sensitizer from non-sensitizer, but may classify known skin sensitizers according to their potency. It assesses the chemical concentration resulting in 50% cytotoxicity (EE-EC50) or 2-fold increase IL-1α (IL-1α2x). Four laboratories received 13 coded sensitizers. Reproducible results were obtained each laboratory. A binary prediction model, EC50 ≥7 mg/ml = weak moderate and <7 strong extreme had an accuracy of 77%. superior EE (EC50 IL-1α2x) correlation was...
The neurotrophins are a family of proteins that promote neuronal survival and neurite outgrowth during development can also enhance the regeneration injured adult neurons. local continuous delivery these at site injury is problematic, since this requires repeated intraparenchymal injections or use invasive canula-micropump devices. In present study we report generation characterization an adenoviral vector for member neurotrophins, neurotrophin-3 (Ad-NT-3). Using Ad-NT-3, examined expression...
Adverse Outcome Pathways (AOPs) are conceptual frameworks that tie an initial perturbation (molecular initiating event) to a phenotypic toxicological manifestation (adverse outcome), through series of steps (key events). They provide therefore standardized way map and organize mechanistic information. As such, AOPs inform on key events underlying toxicity, thus supporting the development New Approach Methodologies (NAMs), which aim reduce use animal testing for toxicology purposes. However,...
The kinetic responses of leukocyte subsets to exercise and their recovery may serve as indicators immunological resilience. These time-dependent were investigated in healthy young men using a bicycle ergometer test. Fifteen recreationally active male cyclists (20–35 years, VO2max 56.9 ± 3.9 mL kg−1 min−1) performed four protocols with 1 h duration cross-over design: at 70% the maximal workload (Wmax) hydrated mildly dehydrated state, 50% Wmax, intermittently 85/55% Wmax blocks 2 min. numbers...
Objective To develop and internally validate a prognostic model to predict chronic pain after new episode of acute or subacute non-specific idiopathic, non-traumatic neck in patients presenting physiotherapy primary care, emphasising modifiable biomedical, psychological social factors. Design A prospective cohort study with 6-month follow-up between January 2020 March 2023. Setting 30 care practices. Participants Patients presentation pain, duration lasting no longer than 12 weeks from...
The first ONTOX Hackathon of the EU Horizon 2020-funded project was held on 21-23 April 2024 in Utrecht, Netherlands (https://ontox-project.eu/hackathon/). This participatory event aimed to collectively advance innovation for human safety through use Artificial Intelligence (AI), and hence significantly reduce reliance animal-based testing. Expert scientists, industry leaders, young investigators, members animal welfare organisations academics alike, joined hackathon. Eight teams were...
To study the ways in which compounds can induce adverse effects, toxicologists have been constructing Adverse Outcome Pathways (AOPs). An AOP be considered as a pragmatic tool to capture and visualize mechanisms underlying different types of toxicity inflicted by any kind stressor, describes interactions between key entities that lead outcome on multiple biological levels organization. The construction or optimization an is labor intensive process, currently depends manual search,...
Implementation of reliable methodologies allowing Reduction, Refinement, and Replacement (3Rs) animal testing is a process that takes several decades still not complete. Reliable methods are essential for regulatory hazard assessment chemicals where differences in test protocol can influence the outcomes thus affect confidence predictive value organisms used as an alternative mammals. Although guidelines common mammalian studies, they scarce non-vertebrate would allow 3Rs testing. Here, we...