A5036702193- Contact Dermatitis and Allergies
- Occupational exposure and asthma
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immunotoxicology and immune responses
- Advancements in Transdermal Drug Delivery
- Animal testing and alternatives
- Wound Healing and Treatments
- Allergic Rhinitis and Sensitization
- 3D Printing in Biomedical Research
- Dermatologic Treatments and Research
- Dermatology and Skin Diseases
- Ocular Surface and Contact Lens
- Tattoo and Body Piercing Complications
- Tissue Engineering and Regenerative Medicine
- Skin Protection and Aging
- Silk-based biomaterials and applications
- Pesticide Exposure and Toxicity
- Periodontal Regeneration and Treatments
- Diagnosis and Treatment of Venous Diseases
- Hair Growth and Disorders
- Cell Adhesion Molecules Research
- Pressure Ulcer Prevention and Management
- vaccines and immunoinformatics approaches
- Agricultural safety and regulations
Amsterdam University Medical Centers
2020-2024
Vrije Universiteit Amsterdam
2008-2024
University Medical Center
2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2010-2020
University of Twente
2014
National Institute for Public Health and the Environment
1999-2002
ABSTRACT Full‐skin substitutes, epidermal and dermal substitutes are currently being used to heal deep burns chronic ulcers. In this study, we investigated which wound‐healing mediators released from these constructs whether keratinocyte–fibroblast interactions involved. Autologous skin were constructed human keratinocytes, fibroblasts, acellular donor dermis. Full‐thickness was represent an autograft. Secretion of by means protein array, enzyme‐linked immunosorbent assay, neutralizing...
In this report, the construction of a functional, immunocompetent, full-thickness skin equivalent (SE) is described, consisting an epidermal compartment containing keratinocytes, melanocytes, and human LCs derived from MUTZ-3 cell line (MUTZ-LC) fibroblast-populated dermal compartment. The CD1a(+)Langerin(+)HLA-DR(+) MUTZ-LCs populate entire epidermis at similar density to that found in native skin. Exposure SE subtoxic concentrations allergens NiSO(4) resorcinol resulted LC migration out...
Organotypic models make it possible to investigate the unique properties of oral mucosa in vitro. For gingiva, use human primary keratinocytes (KC) and fibroblasts (Fib) is limited due availability size donor biopsies. The physiologically relevant immortalized cell lines would solve these problems. aim this study was develop fully differentiated gingiva equivalents (GE) constructed entirely from lines, compare them with counterpart (Prim), test relevance an vitro wound healing assay....
After allergen or irritant exposure, Langerhans cells (LC) undergo phenotypic changes and exit the epidermis. In this study we describe unique ability of MUTZ-3 derived (MUTZ-LC) to display similar plasticity as their primary counterparts when incorporated into a physiologically relevant full-thickness skin equivalent model (SE-LC). We differences similarities in mechanisms regulating LC migration upon exposure. The consisted reconstructed epidermis containing differentiated keratinocytes...
The immune system is called into action by alarm signals generated from injured tissues. We examined the nature of these after exposure skin residential cells to contact allergens (nickel sulfate and potassium dichromate) a irritant [sodium dodecyl (SDS)]. Nickel sulfate, dichromate, SDS were applied topically stratum corneum human equivalents. A similar concentration-dependent increase in chemokine (CCL20, CCL27, CXCL8) secretion was observed for all three chemicals. Exposure nickel...
Background Chronic wounds represent a major problem to our society. Therefore, advanced wound‐healing strategies for the treatment of these are expanding into field tissue engineering. Objectives To develop novel tissue‐engineered, autologous, full‐thickness skin substitute entirely human origin and determine its ability heal chronic wounds. Methods Skin substitutes (fully differentiated epidermis on fibroblast‐populated dermis) were constructed from 3‐mm punch biopsies isolated patients be...
Background/purpose: Cytokines play an important role in inflammatory and repair processes occurring the skin. The objectives of this study were to determine amounts cytokines protein isolated by tape stripping different layers stratum corneum (SC), compare normal skin with exposed vivo irritant sodium lauryl sulphate (SLS). Methods: In eight volunteers, we determined amount total soluble also interleukin‐1α (IL‐1α) pooled strips obtained from upper, intermediate lower parts SC. Three types...
Both oral mucosa and skin have the capacity to maintain immune homeostasis or regulate responses upon environmental assault. Whereas much is known about key innate events in skin, little mucosa. Comparative studies are limited due scarce supply of for ex vivo studies. Therefore, we used organotypic tissue equivalents (reconstructed epithelium on fibroblast-populated collagen hydrogel) study cross talk between cells. Oral were compared regarding secretion cytokines chemokines involved LC...
Application of reconstructed human Skin (RhS) is a promising approach for the treatment extensive wounds and drug efficacy safety testing. However, incorporating appendages, such as hair follicles, into RhS still remains challenge. The follicle plays critical role in thermal regulation, dispersion sweat sebum, sensory tactile functions, skin regeneration, repigmentation. aim this study was to determine whether neopapilla could be incorporated (differentiated epidermis on fibroblast...
Abstract Integration of reconstructed human skin (RhS) into organ‐on‐chip (OoC) platforms addresses current limitations imposed by static culturing. This innovation, however, is not without challenges. Microfluidic devices, while powerful, often encounter usability, robustness, and gas bubble issues that hinder large‐scale high‐throughput setups. study aims to develop a novel re‐usable multi‐well microfluidic adaptor (MMA) with the objective provide flexible tool for biologists implementing...
During the last decade, number of people with ≥1 tattoo has increased noticeably within European population. Despite this, limited safety information is available for inks.To test skin sensitization potential 5 inks in vitro by using reconstructed human (RHS) and contact biomarker interleukin (IL)-18.Two red 3 black inks, 1 additive (Hamamelis virginiana extract) irritant control (lactic acid) were tested. The culture medium RHS (reconstructed epidermis on a fibroblast-populated collagen...
Abstract: At this moment, pressure ulcer risk assessment is dominated by subjective measures and does not predict development satisfactorily. Objective are, therefore, needed for an early detection of these ulcers. The current in vitro study evaluates cytokines chemokines [interleukin 1 α (IL‐1 ), interleukin receptor antagonist (IL‐1RA), tumor necrosis factor (TNF‐ ) 8 (CXCL8/IL‐8)] as markers mechanically‐induced epidermal damage. Various degrees damage were induced subjecting commercially...
According to the new EU Medical Devices (MDR) legislation coming into effect in 2017, manufactures will have comply with higher standards of quality and safety for medical devices order meet common concerns regarding such products. Metal alloys are extensively used dentistry medicine (e.g. orthopedic surgery cardiology) even though clinical experience suggests that many metals sensitizers. The aim this study was further test applicability domain vitro reconstructed human epidermis (RhE)...