A5066666658- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Polyamine Metabolism and Applications
- Animal testing and alternatives
- DNA Repair Mechanisms
- Wound Healing and Treatments
- CRISPR and Genetic Engineering
- Nanoparticles: synthesis and applications
- Hair Growth and Disorders
- Cannabis and Cannabinoid Research
- Municipal Solid Waste Management
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Electrospun Nanofibers in Biomedical Applications
- Polysaccharides and Plant Cell Walls
- Healthcare and Environmental Waste Management
- Telomeres, Telomerase, and Senescence
- Microplastics and Plastic Pollution
- Pesticide Exposure and Toxicity
- DNA and Nucleic Acid Chemistry
- Heavy Metal Exposure and Toxicity
- Chemical Safety and Risk Management
Agency for Science, Technology and Research
2018-2024
Nanyang Technological University
2022-2024
Institute of Medical Biology
2018
National University of Singapore
2012-2017
Background Investigating the cellular and molecular signatures in eukaryotic cells following exposure to nanoparticles will further our understanding on mechanisms mediating nanoparticle induced effects. This study illustrates effects of silver (Ag-np) normal human lung cells, IMR-90 brain cancer U251 with emphasis gene expression, induction inflammatory mediators interaction Ag-np cytosolic proteins. Results We report that are capable adsorbing proteins their surface may influence function...
Silver nanoparticles (Ag-np) have been used in medicine and commercially due to their anti-microbial properties. Therapeutic potentials of these are being explored extensively despite the lack information on mechanism action at molecular cellular level. Here, we investigated DNA damage response repair following Ag-np treatment mammalian cells. Studies shown that exerts genotoxicity through double-strand breaks (DSBs). DNA-PKcs, catalytic subunit dependent protein kinase, is an important...
Patients suffering from brain tumours such as glioblastoma and medulloblastoma have poor prognosis with a median survival of less than year. Identifying alternative molecular targets would enable us to develop different therapeutic strategies for better management these tumours. Glioblastoma (MO59K KNS60) cells (ONS76) were used in this study. Telomerase inhibitory effects MST-312, chemically modified-derivative epigallocatechin gallate, the assessed using telomere repeat amplification...
The micronucleus (MN) assay is widely used as part of a battery tests applied to evaluate the genotoxic potential chemicals, including new food additives and novel ingredients. Micronucleus assays typically utilise homogenous in vitro cell lines which poorly recapitulate physiology, biochemistry genomic events gut, site first contact for ingested materials. Here we have adapted validated MN endpoint protocol use with complex 3D reconstructed intestinal microtissues; named this intestine...
Abstract The aim of the present study was to evaluate compatibility reconstructed 3D human small intestinal microtissues perform in vitro comet assay. assay is a common follow-up genotoxicity test confirm or supplement other data. Technically, it can be performed utilizing range and vivo systems. Here, we have developed new (RICom) protocol for assessment orally ingested materials. intestine major site food digestion adsorption, first-pass metabolism as well an early toxicant first contact...
<title>Abstract</title> While valuable for hazard identification, <italic>in vitro</italic> genotoxicity tests that are conducted in 2D monolayer cell cultures possess limitations to accurately envisage vivo</italic> outcomes and may result unnecessary follow-up studies. One limitation is their relevance actual human exposure the assessment of tissues representative “sites-of-first-contact” with genotoxic agents. The revised OECD testing guidelines had emphasized need considering...