The UV components of sunlight are believed to be a major cause human skin cancer, and DNA is thought the principal molecular target. Alterations intensity wavelength distribution solar radiation reaching surface earth, for example by depletion stratospheric ozone, will change effectiveness in damaging skin. Evaluation magnitude such effects requires knowledge altered spectrum action We have determined an frequency pyrimidine dimer formation induced per unit dose incident on surface. peak...

Although DNA absorption at wavelengths greater than 300 nm is much weaker that shorter wavelengths, this seems to be responsible for of the biological damage caused by solar radiation less 320 nm. Accurate measurement spectrum above complicated turbidity characteristic concentrated solutions DNA. We have measured spectra from calf thymus, Clostridium perfringens, Escherichia coli, Micrococcus luteus, salmon testis, and human placenta using procedures which separate optical density due true...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOptical spectra of oxy- and deoxyhemoglobinWilliam A. Eaton, Louise Karle Hanson, P. J. Stephens, C. Sutherland, B. R. DunnCite this: Am. Chem. Soc. 1978, 100, 16, 4991–5003Publication Date (Print):August 1, 1978Publication History Published online1 May 2002Published inissue 1 August 1978https://pubs.acs.org/doi/10.1021/ja00484a013https://doi.org/10.1021/ja00484a013research-articleACS PublicationsRequest reuse permissionsArticle...

Sutherland, B. M., Bennett, P. V., J. C. and Laval, Clustered DNA Damages Induced by X Rays in Human Cells. Radiat. Res. 157, 611–616 (2002).Although DSBs are known to be important producing the damaging effects of ionizing radiation cells, bistranded clustered damages—two or more oxidized bases, abasic sites strand breaks on opposing strands within a few helical turns—are postulated difficult repair thus critical radiation-induced lesions. Gamma rays can induce damages solution, high-energy...

New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at new CD1 dedicated SRCD beamline ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on flux also focal spot size light. Comparisons with similar studies other facilities worldwide lead to estimation a...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDNA photoreactivating enzyme from placental mammals. Origin and characteristicsBetsy M. Sutherland, Paul Runge, John C. SutherlandCite this: Biochemistry 1974, 13, 23, 4710–4715Publication Date (Print):November 1, 1974Publication History Published online1 May 2002Published inissue 1 November 1974https://doi.org/10.1021/bi00720a005Request reuse permissionsArticle Views61Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the...

We have measured the magnetic circular dichroism of ferrocytochrome c from 350 to 1000 nm and MCD ferricytochrome 750 nm. present expressions for shapes absorption spectra in presence a field randomly oriented molecules general case which band is split zero field, two components different intensities. After correcting effects unresolved zero-field splitting, we find that ``angular momentum'' Q00 Fe(II) cyt 7.9 μB. show 23-fold decrease upon oxidation Fe(III) due about fivefold increased...

Abstract The organization of pigment‐protein complexes into large chiral macrodomains was investigated in wild‐type and chlorophyll b ‐less mutant thylakoid membranes barley. variations the anomalous circular dichroism bands angular‐dependence intensity differential scattering showed that chloroplasts, formation governed by interactions light‐harvesting alb (LHCII). Two external factors could be identified which regulate parameters signal: (i) electrostatic screening divalent cations under...

Predicting the effects of polychromatic light on biological systems is a central goal environmental photobiology. If dose–response function for process linear incident system at each wavelength within spectrum, effect spectrum obtained by integrating product cross section reaction and spectral irradiance that over both time. This procedure cannot be used, however, if functions an are not photon dose. Although many photochemical reactions biologically relevant range doses, end points not. I...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTime-resolved fluorescence and proton NMR studies of tyrosine analogs: correlation NMR-determined rotamer populations kineticsWilliam R. Laws, J. B. Alexander Ross, Herman Wyssbrod, Joseph M. Beechem, Ludwig Brand, John Clark SutherlandCite this: Biochemistry 1986, 25, 3, 599–607Publication Date (Print):February 11, 1986Publication History Published online1 May 2002Published inissue 11 February...

Paranasal sinusitis occurred in 52 immunosuppressed cancer patients treated over 5 years at the University of Maryland Cancer Center. Twenty-one had aspergillus sinusitis; Aspergillus sp, including flavus and niger were directly recovered from sinus 19 21 infections. Two other with involvement positive nose cultures for or fumigatus microbiologically documented pulmonary aspergillosis considered clinically, although not microbiologically, documented. Predisposing factors during 60 days prior...

Journal Article Immune Complex Disease in the Kidneys of Lymphoma-Leukemia Patients: The Presence an Oncornavirus-Related Antigen Get access John C. Sutherland, Sutherland Section Immunology and Cell Biology, Baltimore Cancer Research Center, National Institute, Department Pathology, U.S.P.H.S. Hospital,4Baltimore, Maryland 21211 Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael R. Mardiney, Jr. JNCI: Volume 50, Issue 3, March 1973, Pages 633–644,...

Pericardial effusions following radiotherapy for Hodgkins Disease have previously been described as infrequent and related to the total dose of radiation received. Analysis all chest x-rays on 81 patients who received upper-mantle at Baltimore Cancer Research Center between 1968 1972 disclosed an incidence pericardial 30.9% (25 81), with 13.6% (11 81) requiring limitation activity (5) or pericardiectomy (6). Clinical presentation radiation-related percardial was subtle, signs symptoms a late...

SUMMARYA case of central nervous system chloromas has been presented, along with the pathological and clinical manifestations this tumor its association acute leukemia. This is unique since it first reported intracranial preceding bone marrow evidence leukemia by one year.

Ultraviolet radiation in sunlight damages DNA plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels cyclobutyl pyrimidine dimers measured by excision photoreactivation. By using alkaline gel electrophoresis nonradioactive DNAs treated a dimer-specific endonuclease, we quantitated ethidium-stained electronic imaging calculated frequencies from number...