Anthraquinone compounds have been shown to induce apoptosis in different cancer cell types. Effects of chrysophanol, an anthraquinone compound, on death not well studied. The goal this study was examine if chrysophanol had cytotoxic effects and such involved or necrosis J5 human liver cells. Chrysophanol induced cells a dose- time-dependent manner. Non-apoptotic by characterized caspase independence, delayed externalization phosphatidylserine plasma membrane disruption. Blockage apoptotic...

Anthraquinones have been shown to induce apoptosis in different types of tumor cells, but the mechanisms danthron-induced cytotoxicity and human gastric cancer cells not adequately explored. This study investigated roles caspase cascades, ROS, DNA damage, mitochondrial disruption, Bax Bcl-2 proteins SNU-1 a commonly used cell culture system for vitro studies. Cells were incubated with concentrations danthron time- and/or dose-dependent manner. Cell morphological changes (shrinkage rounding)...

Bronchial hyperresponsiveness to contractile agonists and nonspecific irritants is a characteristic feature of bronchial asthma. The mechanisms causing this hyperirritability are unknown. existence separate receptors for leukotrienes C4 D4 (LTC4 LTD4) has been demonstrated previously by physiologic radioligand binding studies. rank order potency the sulfidopeptide contracting tracheal spirals [leukotriene E4 (LTE4) greater than LTD4 = LTC4] different from that parenchymal strips (LTD4 LTE4...

Human leukocytes generate platelet-activating factor (PAF-acether), a lipid mediator of inflammation, from membrane alkyl phospholipids through the release arachidonic acid or other fatty acids at 2-position and subsequent acetylation. Because it was previously demonstrated that fish oil suppress human leukocyte metabolism, separate experiments were conducted to investigate effects dietary supplementation in vitro incubation with on calcium ionophore-stimulated PAF-acether generation...

Adjacent segment disease is an important complication after fusion of degenerative lumbar spines. However, the role body mass index (BMI) in adjacent has been addressed less.To examine relationship between BMI and for spine diseases.For this retrospective study, we enrolled 190 patients undergoing surgery degeneration. at admission was documented. defined by integration clinical presentations radiographic criteria based on morphology dural sac magnetic resonance images.Adjacent identified 13...

This study investigated whether there are marked differences in surface markers between rabbit and human mesenchymal stem cells (MSCs). Murine MSCs have been reported to be CD90-negative. Rat CD71-negative. Our previous also shows that CD29-negative. However, generally considered CD29-, CD71-, CD90-positive. Therefore, the of might differ from those other species. Rabbit bone marrow were obtained had a multi-differentiation potential. The phenotype these was studied using flow cytometry...

Dysfunction of mitochondria causes defects in oxidative phosphorylation system (OXPHOS) and increased production reactive oxygen species (ROS) triggering the activation cell death pathway that underlies pathogenesis aging various diseases. The process autophagy to degrade damaged cytoplasmic components as well dysfunctional is essential for ensuring survival. We analyzed role inpatient-specific induced pluripotent stem (iPS) cells generated from fibroblasts patients with mitochondrial...

Varicocele is recognized as a cause of male infertility. Testis hypoxia may be one the possible mechanisms varicocele. We examined whether tissue occurred in ISV patients with varicocele by detecting expression HIF-1alpha.The study group consisted 8 grade 3 left The control 6 volunteers indirect inguinal hernia. Using surgical incision, 1 cm section was resected from each patient both groups specimens for immunoblotting and immunohistochemical staining HIF-1alpha. Results were analyzed using...

Background: Delayed contralateral epidural hematoma (EDH) after decompressive surgery for acute subdural (SDH) is uncommon. If unrecognized, this delayed can cause devastating consequences. We present our experience with group of patients and discuss the diagnosis management dangerous condition. Methods: This study included 12 traumatic SDH who developed EDH evacuation. Clinical radiographic information was obtained through a retrospective review medical records radiographs. Results: There...

Environmental hypoxia is a common challenge that many aquatic organisms experience in their habitat. Responding to requires metabolic reprogramming so energy-demanding processes are regulated match available energy reserves. In this study we explored the transcriptional control of reorganization liver hypoxia-tolerant burrow-dwelling goby, Gillichthys mirabilis. Gene expression data revealed pathways associated with triglyceride hydrolysis were upregulated by whereas synthesis downregulated....

Decompressive craniectomy is a life-saving measure for patients who have sustained traumatic brain injury (TBI), but undergoing this procedure may still die during an early phase of head injury. The aim study was to investigate the incidence, causes, and risk factors 30-day mortality in traumatically brain-injured decompressive craniectomy.The authors included 201 head-injured 3-year retrospective study. main outcome evaluated had undergone after TBI. Demographic clinical data, including...

The present study was to explore the biological responses of newly compound, MJ-29 in murine myelomonocytic leukemia WEHI-3 cells vitro and vivo fates. We focused on effects ER stress mitochondria-dependent apoptotic death cells, hypothesize that might fully impair orthotopic leukemic mice. Our results indicated a concentration-dependent decrease cell viability shown MJ-29-treated cells. DNA content examined utilizing flow cytometry, whereas populations were determined using annexin V/PI,...

Milkfish (Chanos chanos), an important marine aquaculture species in southern Taiwan, show considerable euryhalinity but have low tolerance to sudden drops water temperatures winter. Here, we used high throughput next-generation sequencing (NGS) identify molecular and biological processes involved the responses environmental changes. Preliminary tests revealed that seawater (SW)-acclimated milkfish tolerated lower than fresh (FW)-acclimated group. Although FW- SW-acclimated different levels...

Amphibians are highly susceptible to osmotic stress but, nonetheless, some species can adapt locally withstand moderately high levels of salinity. Maintaining the homeostasis body fluids by efficient osmoregulation is thus critical for larval survival in saline environments. We studied role acclimation increased physiological tolerance elevated water salinity Indian rice frog (Fejervarya limnocharis) tadpoles exposed brackish water. quantified effects on tadpole survival, osmolality,...