A5033199845- Renal Diseases and Glomerulopathies
- Epigenetics and DNA Methylation
- Carbon and Quantum Dots Applications
- Hedgehog Signaling Pathway Studies
- Chronic Kidney Disease and Diabetes
- Genetic and Kidney Cyst Diseases
- Advanced biosensing and bioanalysis techniques
- Nanocluster Synthesis and Applications
- Hippo pathway signaling and YAP/TAZ
- Renal and related cancers
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Assisted Reproductive Technology and Twin Pregnancy
- Acute Kidney Injury Research
- Lysosomal Storage Disorders Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Vasculitis and related conditions
- Complement system in diseases
- Endoplasmic Reticulum Stress and Disease
- Ion Transport and Channel Regulation
Shanghai Medical College of Fudan University
2016-2025
Northwest Normal University
2023-2025
Binzhou University
2018-2024
Binzhou Medical University
2016-2024
Fudan University
2012-2024
Shanxi University
2017-2024
Sichuan University
2023
West China Second University Hospital of Sichuan University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Zhongshan Hospital
2012-2023
The Hedgehog (Hh) signal is transduced across the membrane by heptahelical protein Smoothened (Smo), a developmental regulator, oncoprotein and drug target in oncology. We present 2.3 Å crystal structure of extracellular cysteine rich domain (CRD) vertebrate Smo show that it binds to oxysterols, endogenous lipids activate Hh signaling. oxysterol-binding groove CRD analogous used Frizzled 8 bind palmitoleyl group Wnt ligands similar pockets other Frizzled-like CRDs hydrophobic ligands....
CXC chemokines and their cognate receptors have been implicated widely in cancer pathogenesis. In this study, we report a critical causal relationship between CXCR6 expression tumorigenesis the setting of human hepatocellular carcinoma (HCC). Among chemokine receptors, only was detected all hepatoma cell lines studied. Moreover, HCC tissue, significantly higher than noncancerous liver tissues. Reduction or its ligand CXCL16 cells reduced invasion vitro tumor growth, angiogenesis, metastases...
In mammals, the homeodomain transcription factor Prox1 acts as central regulator of lymphatic cell fate. Its restricted expression in a subset cardinal vein cells leads to switch towards specification and hence represents prerequisite for initiation lymphangiogenesis. Murine Prox1-null embryos lack structures, sustained is indispensable maintenance fate even at adult stages, highlighting unique importance this gene lineage. Whether pre-eminent role within vasculature conserved other...
Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), a potent immunoregulatory molecule, can down-regulate T-cell activation and inhibit anti-tumor immune response. This study showed that LPS-stimulated human dendritic cells (DCs) decreased the expression of HLA-DR, CD83 costimulatory molecules (CD40, CD80 CD86) following coculturing with CTLA-4+ breast cancer cells. Moreover, suppressed DCs further inhibited proliferation allogeneic CD4+/CD8+ T-cells, differentiation Th1 function cytotoxic...
Abstract Acute kidney injury (AKI) is associated with significant morbidity and its chronic inflammation contributes to subsequent disease (CKD) development. Yes-associated protein (YAP), the major transcriptional coactivator of Hippo pathway, has been shown inflammation, but role mechanism in AKI-CKD transition remain unclear. Here we aimed investigate YAP AKI-induced inflammation. Renal ischemia/reperfusion (I/R) was used induce a mouse model transition. We verteporfin (VP),...
Glutathione (GSH), a cancer-related biomarker in cells, has numerous important roles maintaining intracellular homeostasis; hence, rapid detection and imaging of GSH are essential physiological pathological processes. However, because other biological thiols, such as cysteine (Cys) homocysteine (Hcy), very similar structure properties to GSH, it remains challenge distinguish from Cys Hcy, particularly vivo. Herein, novel ratiometric fluorescent probe (NSCDs@Hg2+) was designed discriminate...
Loss of function mutations Kif7, the vertebrate orthologue Drosophila Hh pathway component Costal2, cause defects in limbs and neural tubes mice, attributable to ectopic expression target genes. While this implies a functional conservation Cos2 Kif7 between flies vertebrates, association with primary cilium, an organelle absent from most cells, suggests their mechanisms action may have diverged. Here, using mutant alleles induced by Zinc Finger Nuclease-mediated targeted mutagenesis, we show...
Abstract The G‐protein‐coupled receptor kinase 2 (adrbk2/ GRK 2) has been implicated in vertebrate Hedgehog (Hh) signalling based on the effects of its transient knock‐down mammalian cells and zebrafish embryos. Here, we show that response to Hh is effectively abolished absence Grk2 activity. Zebrafish embryos lacking all activity are refractory both Sonic hedgehog (Shh) oncogenic Smoothened (Smo) activity, but remain responsive inhibition cAMP‐dependent protein ( PKA ) Mutation domain...
Nod-like receptor protein 3 (NLRP3), as an inflammatory regulator, has been implicated in acute kidney injury (AKI). Failed recovery after AKI can lead to chronic disease (CKD). However, the role of NLRP3 AKI-CKD transition is still unknown. A mild or severe mouse model was performed by using ischemia-reperfusion (IRI). We evaluated renal expression and phases ischemic AKI, respectively. Although serum creatinine (Cr) blood urea nitrogen (BUN) levels phase were equivalent normal baseline,...
Abstract If optical probes with different sensitivity could be performed simultaneously in their hard states (e.g., film), soft gel), and solution states, sensing accuracy enhanced due to the highly mutual calibration. The biomass of cellulose nanocrystals is less compatible commonly used organo‐fluorescent dyes require chemical modification improve application performance, but this leads less‐effective material cost. In work, depending on electrostatic attractions between negatively charged...