A5008461707- Tryptophan and brain disorders
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cardiac Valve Diseases and Treatments
- Acute Myocardial Infarction Research
- Hepatitis Viruses Studies and Epidemiology
- Coronary Interventions and Diagnostics
- Immune cells in cancer
- Antiplatelet Therapy and Cardiovascular Diseases
- Infective Endocarditis Diagnosis and Management
- Viral gastroenteritis research and epidemiology
- Connexins and lens biology
- Electrolyte and hormonal disorders
- Peripheral Artery Disease Management
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- RNA regulation and disease
- Liver Disease and Transplantation
- Atrial Fibrillation Management and Outcomes
- Renal and Vascular Pathologies
- Ethnobotanical and Medicinal Plants Studies
- Aortic Disease and Treatment Approaches
- Cardiac Imaging and Diagnostics
Indian Institute of Science Education and Research Kolkata
2022-2024
Augusta University
2007-2021
University of Delhi
2021
MemorialCare Health System
2020-2021
Icahn School of Medicine at Mount Sinai
2017-2020
Cardiovascular Institute of the South
2017-2019
Augusta University Health
2008-2018
Mount Sinai Hospital
2018
Mount Sinai Health System
2017
Mount Sinai Medical Center
2014-2017
Antigen-presenting cells (APCs) can induce tolerance or immunity. We describe a subset of human APCs that express indoleamine 2,3-dioxygenase (IDO) and inhibit T cell proliferation in vitro. IDO-positive constituted discrete identified by coexpression the cell-surface markers CD123 CCR6. In dendritic (DC) lineage, IDO-mediated suppressor activity was present fully mature as well immature CD123+ DCs. IDO+ DCs could also be readily detected vivo, which suggests these may represent regulatory humans.
One mechanism contributing to immunologic unresponsiveness toward tumors may be presentation of tumor antigens by tolerogenic host APCs. We show that mouse tumor-draining LNs (TDLNs) contained a subset plasmacytoid DCs (pDCs) constitutively expressed immunosuppressive levels the enzyme indoleamine 2,3-dioxygenase (IDO). Despite comprising only 0.5% LN cells, these pDCs in vitro potently suppressed T cell responses presented themselves and also, dominant fashion, third-party nonsuppressive...
A small population of plasmacytoid DCs (pDCs) in mouse tumor-draining LNs can express the immunoregulatory enzyme indoleamine 2,3-dioxygenase (IDO). We show that these IDO+ pDCs directly activate resting CD4+CD25+Foxp3+ Tregs for potent suppressor activity. In vivo, isolated from were constitutively activated and suppressed antigen-specific T cells immediately ex vivo. vitro, rapidly non-tumor-bearing hosts without need mitogen or exogenous anti-CD3 crosslinking. Treg activation by was MHC...
Abstract Indoleamine 2,3-dioxygenase (IDO) is an immunosuppressive enzyme that contributes to tolerance in a number of biological settings. In cancer, IDO activity may help promote acquired tumor antigens. The inhibitor 1-methyl-tryptophan being developed for clinical trials. However, exists two stereoisomers with potentially different properties, and it has been unclear which isomer might be preferable initial development. this study, we provide evidence the d l exhibit important cell...
Abstract Human monocyte-derived dendritic cells (DCs) are capable of expressing the tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO), which allows them to suppress Ag-driven proliferation T in vitro. In DCs that express IDO, activity is tightly regulated, with protein being constitutively expressed, but functional requiring an additional set triggering signals supplied during Ag presentation. We now show IDO obligately requires ligation B7-1/B7-2 molecules on by CTLA4/CD28...
TLR ligands are effective vaccine adjuvants because they stimulate robust proinflammatory and immune effector responses abrogate suppression mediated by regulatory T cells (Tregs). Paradoxically, systemic administration of high doses CpGs that bind to TLR9 stimulated Tregs in mouse spleen acquire potent suppressor activity dependent on interactions between programmed death-1 its ligands. This response CpG treatment manifested 8-12 h was a rare population plasmacytoid dendritic (CD19(+) pDC)...
The agent(s) responsible for sporadic non-A, non-B hepatitis in humans was serially transmitted rhesus monkeys by intravenous inoculation of the stool extract from a patient. A novel agent called HFV (hepatitis French [origin] virus) present as 27- to 37-nm particles infectious extract. Hepatopathic lesions were noticed infected during acute phase illness. purified viral consist double-stranded DNA approximately 20 kb and are detected monkey liver. Analysis cell culture detects 20-kb-long...
Topical application of phorbol myristate acetate (PMA) elicits intense local inflammation that facilitates outgrowth premalignant lesions in skin after carcinogen exposure. The inflammatory response to PMA treatment activates immune stimulatory mechanisms. However, we show here exposure also induces plasmacytoid dendritic cells (pDCs) draining lymph nodes (dLNs) express indoleamine 2,3 dioxygenase (IDO), which confers T cell suppressor activity on pDCs. induced IDO-mediated inhibitory this...
At sites of inflammation, certain regulatory T cells (Treg cells) can undergo rapid reprogramming into helper-like without loss the transcription factor Foxp3. We show that is controlled by downregulation Eos (Ikzf4), an obligate corepressor for Reprogramming was restricted to a specific subset "Eos-labile" Treg present in thymus and identifiable characteristic surface markers DNA methylation. Mice made deficient this became impaired their ability provide help presentation new antigens naive...
The tumor microenvironment is profoundly immunosuppressive. We show that multiple types create intratumoral immune suppression driven by a specialized form of regulatory T cell (Treg) activation dependent on the PTEN (phosphatase and tensin homolog) lipid phosphatase. acted to stabilize Tregs in tumors, preventing them from reprogramming into inflammatory effector cells. In mice with Treg-specific deletion PTEN, tumors grew slowly, were inflamed, could not an immunosuppressive...
Meeting abstracts The tumor microenvironment is profoundly immunosuppressive, but exactly how this coordinated and maintained remains poorly understood. We show that multiple transplantable autochthonous mouse tumors actively elicit a population of highly suppressive regulatory T cells (
<h3>Importance</h3> Women with acute myocardial infarction (MI) undergoing mechanical reperfusion remain at increased risk of adverse cardiac events and mortality compared their male counterparts. Whether the benefits new-generation drug-eluting stents (DES) are preserved in women MI remains unclear. <h3>Objective</h3> To investigate long-term safety efficacy DES vs early-generation MI. <h3>Design, Setting, Participants</h3> Collaborative, international, individual patient-level data...
The extensive use of antibiotics to treat bacterial infections has led the widespread emergence multidrug-resistant (MDR) pathogens, becoming increasingly difficult with currently available antibacterial agents. present study is based on prospecting ethnomedicinal potential Indian plant varieties for treatment MDR bacteria. Plants produce an array diverse pharmacological compounds in defence against microbial pathogens which may be employed as a novel intervention strategy combat human...
Abstract Combination immunotherapy regimens incorporating agents that target more than a single regulatory pathway or immune inhibitory checkpoint can confer marked enhancement of anti-tumor responses when combined with active immunotherapy. We have tested the antitumor effect IDO inhibitor NLG-919 (currently in Phase I clinical trial) combination indoximod (a different currently II trials), chemotherapy, vaccination and/or PD-1/PD-L1/PD-L2 blockade. Here we show highly potent,...