A5020975029- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Virus-based gene therapy research
- Down syndrome and intellectual disability research
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Genomic variations and chromosomal abnormalities
- Viral gastroenteritis research and epidemiology
- Child Nutrition and Feeding Issues
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Archaeological and Geological Studies
- Medieval Architecture and Archaeology
- CAR-T cell therapy research
- Viral Infections and Immunology Research
- Animal Nutrition and Physiology
- Pediatric health and respiratory diseases
- Meat and Animal Product Quality
- Child and Adolescent Health
- Global Educational Reforms and Inequalities
- Genetics and Neurodevelopmental Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Biotin and Related Studies
- Archaeological and Historical Studies
University of Cambridge
2018-2024
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2014-2017
Centro de Investigación Biomédica en Red
2014
Centre for Biomedical Network Research on Rare Diseases
2014
Rutgers New Jersey Medical School
2006
Rutgers, The State University of New Jersey
2006
Abstract Although the subcellular dynamics of RNA and proteins are key determinants cell homeostasis, their characterization is still challenging. Here we present an integrative framework to simultaneously interrogate transcriptome proteome at resolution by combining two methods: localization (LoRNA) a streamlined density-based isotope tagging (dLOPIT) map protein organelles (nucleus, endoplasmic reticulum mitochondria) membraneless compartments (cytosol, nucleolus cytosolic granules)....
Down syndrome (DS) or trisomy 21 is the result of a genetic dosage imbalance that translates in broad clinical spectrum. A major challenge study DS identification functional elements with wide impact on phenotypic alterations. Recently, miRNAs have been recognized as contributors to several disease conditions by acting post-transcriptional regulators plethora genes. Five chromosome (HSA21) found overexpressed individuals and could function key pathophysiology. Interestingly, trisomic Ts65Dn...
Abstract Codon usage adaptation of lytic viruses to their hosts is determinant for viral fitness. In this work, we analyzed the codon adenoviral proteins by principal component analysis and assessed host. We observed a general clustering according function. However, there was significant variation in preference between host-interacting fiber protein rest structural late phase proteins, with non-optimal fiber. To understand impact bias fiber, optimized Adenovirus-5 hexon protein. The...
Systemic treatment of cancer requires tumour-selective therapies that eliminate cells yet preserve healthy tissues from undesired damage. Tumoral transformation is associated with profound effects in translational reprogramming gene expression, such tumour-specific regulation presents an attractive possibility for generating oncoselective therapies. We recently discovered mRNA control by cytoplasmic polyadenylation element-binding proteins (CPEBs) reactivated cancer. Here we present a novel...
// Xavier Bofill-De Ros 1,2,* , Eneko Villanueva 1,* and Cristina Fillat 1,2 1 Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain 2 Centro de Investigación Biomédica en Red Enfermedades Raras (CIBERER), * These authors contributed equally to this work Correspondence to: Fillat, email: Keywords : Oncolytic adenovirus, fiber protein, L5 gene, miRNAs, pancreatic cancer Received October 03, 2014 Accepted January 12, 2015 Published 31, Abstract Tissue-specific...
Abstract Existing methods to analyse RNA localisation are constrained specific RNAs or subcellular niches, precluding the cell-wide mapping of RNA. We present Localisation (LoRNA), which maps, at once, membranous (nucleus, ER and mitochondria) membraneless compartments (cytosol, nucleolus phase-separated granules). Simultaneous interrogation all locations allows system-wide quantification proportional distribution comprehensive analysis dynamics. Moreover, we have re-engineered LOPIT...
Abstract Arming oncolytic adenoviruses with therapeutic transgenes is a well-established strategy for multimodal tumour attack. However, this sometimes leads to unexpected attenuated viral replication and loss of effects, preventing these viruses from reaching the clinic. Previous work has shown that altering codon usage in genes can hamper fitness. Here, we have analysed how transgene impacts activity. We observe that, although optimized codons show high expression levels at first round...
Abstract Current methods for the identification of RNA–protein interactions require a quantity and quality sample that hinders their application, especially dynamic biological systems or when material is limiting. Here, we present new approach to enrich RNA-Binding Proteins (RBPs): Orthogonal Organic Phase Separation (OOPS), which compatible with downstream proteomics RNA sequencing. OOPS enables recovery RBPs free protein, protein-bound RNA, from single in an unbiased manner. By applying...
Abstract Arming oncolytic adenoviruses with therapeutic transgenes is a well-established strategy for multimodal tumour attack. However, this sometimes leads to unexpected attenuated viral replication and loss of effects, preventing these viruses from reaching the clinic. Previous work has shown that altering codon usage in genes can hamper fitness. Here, we have analysed how transgene impacts activity. We observe that, although optimised codons show high expression levels at first round...
Abstract Characterising RNA-protein interaction dynamics is fundamental to understand how bacteria respond their environment. In this study, we have analysed the of 91% Escherichia coli expressed proteome and RNA-interaction properties 271 RNA-binding proteins (RBPs) at different growth phases. We find that 68% RBPs differentially bind RNA across phases characterise 17 previously unannotated as bacterial including YfiF, a ncRNA-binding protein. While these new are mostly present in...
Polymorphic genes located on the X chromosome may account for some of response differences between males and females after injury infection. Due to dosage compensation by X- inactivation, are mosaics as half their cells carry paternal other maternal X. Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked genetic polymorphism in humans higher animals. This study tested whether G6PD could be utilized a potential marker skewing using mutant mouse model. Anti-mouse antibody was...
Noticia de dos fragmentos cerámica con decoración excisa aparecidos en la antigua Rauda.