A5045297162- Antibiotic Resistance in Bacteria
- Olfactory and Sensory Function Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Biochemical Analysis and Sensing Techniques
- Antibiotics Pharmacokinetics and Efficacy
- Advanced Chemical Sensor Technologies
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Biofuel production and bioconversion
- Salmonella and Campylobacter epidemiology
- Enzyme Structure and Function
- RNA modifications and cancer
- Escherichia coli research studies
- Drug-Induced Adverse Reactions
- Hydrocarbon exploration and reservoir analysis
- Antibiotic Use and Resistance
- Hydraulic Fracturing and Reservoir Analysis
- Catalysis for Biomass Conversion
- Advanced biosensing and bioanalysis techniques
- Intramuscular injections and effects
- Aquaculture Nutrition and Growth
- Bacterial Identification and Susceptibility Testing
- Pancreatic function and diabetes
University of York
2018-2024
Due to rising resistance, new antibacterial strategies are needed, including methods for targeted antibiotic release. As targeting vectors, chelating molecules called siderophores that released by bacteria acquire iron have been investigated conjugation antibacterials, leading the clinically approved drug cefiderocol. The use of small-molecule catalysts prodrug activation within cells has shown promise in recent years, and here we investigate siderophore-linked ruthenium prodrugs cells....
Mammals produce volatile odours that convey different types of societal information. In Homo sapiens, this is now recognised as body odour, a key chemical component which the sulphurous thioalcohol, 3-methyl-3-sulfanylhexan-1-ol (3M3SH). Volatile 3M3SH produced in underarm result specific microbial activity, act on odourless dipeptide-containing malodour precursor molecule, S-Cys-Gly-3M3SH, secreted axilla (underarm) during colonisation. The mechanism by these bacteria recognise...
A novel ciprofloxacin–siderophore Trojan Horse antimicrobial was prepared by incorporating key design features of salmochelin, a stealth siderophore that evades mammalian siderocalin capture via its glycosylated catechol units. Assessment the activity conjugate revealed attachment salmochelin mimic resulted in decreased potency, compared to ciprofloxacin, against two Escherichia coli strains, K12 and Nissle 1917, both iron replete deplete conditions. This observation could be attributed...
Pseudomonas aeruginosa PA01 is one of the major causes disease persistence and mortality in patients with lung pathologies, relying on various host metabolites as carbon energy sources for growth. The ict-ich-ccl operon (pa0878, pa0882, pa0883) PAO1 required growth molecule itaconate, a C5-dicarboxylate. However, it not known how itaconate taken up into P. aeruginosa. Here we demonstrate that genetically linked tripartite ATP-independent periplasmic (TRAP) transporter (pa0884-pa0886), which...
The skin microbiome is dominated by a few key genera, among which Staphylococcus one of the most well-characterised. Recent studies have examined roles various species such as S. epidermidis and hominis within broader microbial communities. However, these investigations often rely on isolates from multiple individuals hence, limiting their ability to capture intra-community interactions. In this study, we focused axillary single healthy individual characterise genetic functional diversity...
Membrane bound acyltransferase-3 (AT3) domain-containing proteins are implicated in a wide range of carbohydrate O-acyl modifications, but their mechanism action is largely unknown. O-antigen acetylation by AT3 acetyltransferases Salmonella spp. can generate specific immune response upon infection and influence bacteriophage interactions. This study integrates situ vitro functional analyses two these proteins, OafA OafB (formerly F2GtrC), which display an "AT3-SGNH fused" domain...
Body odour is a characteristic trait of Homo sapiens, however its role in human behaviour and evolution poorly understood. Remarkably, body linked to the presence few species commensal microbes. Herein we discover bacterial enzyme, limited odour-forming staphylococci that are able cleave odourless precursors thioalcohols, most pungent components odour. We demonstrated using phylogenetics, biochemistry structural biology this cysteine-thiol lyase (C-T lyase) PLP-dependent enzyme moved...
While lignocellulose is a promising source of renewable sugars for microbial fermentations, the presence inhibitory compounds in typical lignocellulosic feedstocks, such as furfural, has hindered their utilisation. In Escherichia coli, major route furfural toxicity depletion NADPH pools due to its use substrate by YqhD enzyme that reduces less toxic alcohol form. Here, we examine potential exploiting benzyl dehydrogenases an alternative means provide this same catalytic function but using...
Abstract The movement of acyl groups across biological membranes is essential for many cellular processes. One major family proteins catalysing this reaction are the transferase 3 (AT3) proteins, which form a pore to allow acyl-CoA penetrate membrane transfer onto an extracytosolic acceptor molecule. Recent structures sequence-unrelated human heparan-α-glucosaminide N -acetyltransferase (HGSNAT) support similar transmembrane acyl-group mechanism. Here we demonstrate that both protein...
Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests AMR are necessary. One the most common clinically important forms bacterial is β-lactam antibiotics (e.g., penicillin). This often caused by β-lactamases, which hydrolyze drugs, rendering them ineffective. Current methods for detecting these enzymes require either time-consuming growth assays or antibiotic mimics such...
The intracellular pathogen S. Typhimurium is a leading cause of foodborne illness across the world and known to rely on range virulence factors colonize human host disease. gene coding for one such factor, stm3169, was determined be upregulated upon macrophage entry its disruption reduces survival in macrophage. In this study we characterize STM3169 protein, which forms substrate binding protein (SBP) an uncharacterized tripartite ATP-independent periplasmic (TRAP) transporter. Genome...
Our understanding of the skin microbiome has dramatically improved since pioneering studies and improvements in sequencing technologies. Species genus Corynebacterium are known to form a major part human but most detailed have focussed on other similarly prevalent genera like Staphylococcus Cutibacteria. Prior this study, there were few complete genomes for commensals Corynebacterium, with only 9 available commonly identified species C. tuberculostearicum. In study we explored from single...
Molecular probes typically require structural modifications to allow for the immobilisation or bioconjugation with a desired substrate but effects of these changes are often not evaluated. Here, we set out determine attaching functional handles first-generation cephalosporin. A series cephalexin derivatives was prepared, equipped chemical tethers suitable site-selective conjugation antibiotics functionalised surfaces. The were positioned remotely from β-lactam ring ensure minimal effect...
Microbes that have evolved to live on lignocellulosic biomass face unique challenges in the effective and efficient use of this material as food. The bacterium Shewanella sp. ANA-3 has potential utilize arabinan arabinoxylan, uptake monosaccharide, l -arabinose, derived from these polymers, is known be mediated by a single ABC transporter. We demonstrate substrate binding protein system, GafA Sw , binds specifically -arabinofuranose, which rare furanose form -arabinose found biomass....
Abstract Characterising RNA-protein interaction dynamics is fundamental to understand how bacteria respond their environment. In this study, we have analysed the of 91% Escherichia coli expressed proteome and RNA-interaction properties 271 RNA-binding proteins (RBPs) at different growth phases. We find that 68% RBPs differentially bind RNA across phases characterise 17 previously unannotated as bacterial including YfiF, a ncRNA-binding protein. While these new are mostly present in...
The aim of this paper is to present the application seismic inversion and time depth conversion for prediction overpressure zones in geological media. Contrary conventional approach we do not used V NMO or RMS analysis. Results play an important role absence well information can help risk reduction while drilling.