Abstract Background This study aims to evaluate the effects of two different concentrations topical hyaluronic acid (HA) on postoperative patient discomfort and wound healing palatal donor sites after free gingival graft (FGG) surgery. Methods Thirty‐six patients requiring FGG were randomly assigned into three groups in an examiner‐masked, randomized, controlled clinical trial. After harvesting grafts, 0.2% 0.8% HA gels used test 1 2, respectively. Gels applied protected with periodontal...

The aim of this study was to compare the differences in self-reported oral health attitudes and behaviour between preclinical clinical dental students Turkey using Hiroshima University Dental Behavioural Inventory (HU-DBI).A Turkish version HU-DBI questionnaire with additional 7 questions, totally 27 items, distributed among 1022 students.The response rate 75% (486 278 students). Significantly higher (P=0.000) percentage students, compared worry about colour their teeth, are bothered by...

Background: To our knowledge, the association of five serotypes Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) to new diagnostic classification scheme defined by American Academy Periodontology in 1999 has not yet been described. The goal this study was characterize frequencies A. isolates from various forms periodontitis using both old and classifications determine relationships between serotype age clinical diagnosis. Methods: A total 345 115 culture‐positive subjects...

Background: Actinobacillus actinomycetemcomitans is a major pathogen in aggressive periodontitis. Our objectives were to determine the periodontal status and occurrence of A. family members subjects with –positive periodontitis (AgP) evaluate probability its intrafamilial transmission. Methods: Of 300 screened, 66 (22%) had AgP . Eleven (probands) these met strict inclusion criteria for study. The study population consisted 55 subjects, including probands their (N = 44). Two groups formed...

This study aimed to evaluate the levels of total matrix metalloproteinase-8 (MMP-8), macrophage-activating factors (MAF), macrophage inflammatory protein (MIP)-1α, colony-stimulating factor (M-CSF), interleukin (IL)-34 in saliva, and serum periodontally healthy, periodontitis Stage III Grade B (P-III-B) C (P-III-C) participants compare changes after non-surgical periodontal treatment (NSPT).A non-smoker systemically healthy 65 participants, 20 P-III-B, 25 P-III-C were recruited for study....

Matrix metalloproteinase-9 (MMP-9), total superoxide dismutase (T-SOD) and sirtuin (SIRT)-1 are interrelated molecules linked with oxidative stress periodontitis pathogenesis which may have implications for both the diagnosis of or efficacy periodontal treatment. This study aimed to evaluate salivary serum MMP-9, T-SOD, SIRT-1 levels in stage 3 patients (P-S3) following non-surgical treatment (NSPT). Forty-nine subjects, comprising 17 healthy, 16 P-S3 Grade B (P-S3GB), C (P-S3GC)...

We determined differences in the expression of certain virulence factors between oral Candida dubliniensis and albicans species. In addition, clonal were sought among C. isolates recovered from patients with without compromised immune system. The material comprised 93 clinical yeast originated 40 subjects (1–5 per subject). All 26 46 healthy routine dental clinic patients. Additionally, 21 collected autoimmune polyendocrinopathy–candidosis–ectodermal dystrophy (APECED), who have chronic...

Background: No information exists on periodontitis‐associated subgingival microbiota from Turkey. We determined the occurrence, interspecies relationships, and clonal characteristics for a group of periodontal bacteria in Turkish study population. Methods: Subgingival microbial samples were obtained patients with localized (LAgP, N = 18) or generalized (GAgP, 17) types aggressive periodontitis, chronic periodontitis (GCP, 14), non‐periodontitis subjects (N 20). Culture methods used to...

Actinobacillus actinomycetemcomitans isolates from 356 individuals were screened for identification of serotype e in order to investigate its distribution relation periodontal status. From subjects with e, 1–6 per subject ( n =61) genotyped using arbitrarily primed–polymerase chain reaction (AP‐PCR) and apaH gene polymerase reaction–restriction fragment‐length polymorphism (PCR‐RFLP) analysis determine the genetic heterogeneity within serotype. Furthermore, one strain was tested fermentation...

Background: The Gram‐negative facultatively anaerobic coccobacillus Actinobacillus actinomycetemcomitans is the major pathogen in localized juvenile periodontitis (LJP) and some forms of adult (AP). A. can be grouped into 5 serotypes (a through e) based on differences carbohydrate moiety cell surface lipopolysaccharide. population genetically heterogeneous. Since studies colonization have mostly applied only culture techniques, clonality follow‐up isolates has not been established. Thus, it...

Clonal diversity of subgingival yeast strains was determined in relation to geographical location and coexistence selected periodontal pathogenic bacteria. A total 60 dental patients from Finland, the United States Turkey each contributed five Candida albicans isolates. C. isolates were serotyped using slide agglutination genotyped polymerase chain reaction (PCR) amplification a random sequence primer. In general, study subject yielded belonging same serotype genotype. occurred more...

Background Analysis of gingival crevicular fluid (GCF) samples may give information unattached (planktonic) subgingival bacteria. Our study represents the first one targeting identity bacteria in GCF. Methodology/Principal Findings We determined bacterial species diversity GCF a group periodontitis patients and delineated contributing host-associated factors. Subgingival paper point (PP) from same sites were taken for comparison. After DNA extraction, 16S rRNA genes PCR amplified DNA-DNA...

Poorly-controlled glycemic status in type 2 diabetes mellitus (T2DM) is suggested to play a role the periodontal inflammatory process by aggregating local cytokine response. Our objectives were profile salivary interleukin (IL)-17 and tumor necrosis factor (TNF)-α levels subjects with T2DM examine their relevance for health control levels.Unstimulated whole saliva samples, together full-mouth recordings (plaque index [PI], bleeding on probing [BOP %], gingival [GI], pocket depth [PPD],...

Abstract Objective This split-mouth randomized study aimed to assess efficacy of leucocyte-platelet-rich fibrin (L-PRF) versus connective tissue graft (CTG) in achieving root coverage (RC) for multiple adjacent gingival recessions (MAGRs) throughout 12-month period. Materials and methods The enrolled 59 teeth from 12 patients with Miller Class I MAGRs ≥ 2 mm on bilateral or contralateral sides. Patients were randomly assigned receive coronally advanced flap (CAF) either CTG (control) L-PRF...

Objective:  The aim of the study was to assess difference in oral health attitudes and behavior between dental nursing students.  Methods:  A total 27 item questions, Hiroshima University-Dental Behavior Inventory (HU-DBI) additionally 7 were distributed a 253 students, 94 159 from Marmara University. The participating voluntary based.  Results:  Seventy-one 157 students willing participate study. significantly more concerned about appearance their teeth (P<0.001), gums compared (P<0.001)....

Background: Periodontitis has been linked to increased risk of cardiovascular diseases. Systemic reactions associated with events may depend on characteristics the subgingival microflora in periodontitis. Our objectives were compare numbers cultivable bacteria, composition and clonal distribution Actinobacillus actinomycetemcomitans ( A. ) two groups patients generalized chronic periodontitis (GCP), one an acute myocardial infarction (AMI‐GCP) other without AMI (non–AMI‐GCP). Methods: In...

We investigated whether certain Actinobacillus actinomycetemcomitans clones occur in elevated proportions subgingival flora, and if the relate to other bacteria samples. A total of 121 A. strains from patients with periodontitis were serotyped 60 also genotyped. The divided into three groups two according proportion . samples genotyped cultured for five species. Among strains, serotype b occurred significantly more frequently high‐ ( n =14, &gt;5%, mean=18.09, SD=20.07%) than low‐ =49,...

Aggregatibacter actinomycetemcomitans is divided into 6 serotypes. Occurrence of non-serotypeable strains known, but background reasons are unclear. We hypothesized that represent new serotypes or have altered expression serotype-specific polysaccharide antigen (S-PA). first characterized 311 from 189 individuals using both immunoassay- and PCR-based serotyping. Next, natural human infection rabbit immunization approaches, we clarified whether the phenotypically expressed S-PA. Immunoassay...

The process conditions for recombinant hepatitis B surface antigen (HBsAg) extraction from transgenic potato were examined. effects of temperature, the reducing agent β-mercaptoethanol (BME), and proteinase inhibitors on level antigenic activity recovered HBsAg determined. Sedimentation profiles performed to characterize assembly into virus-like particles. Increasing temperature sample about 1 min increased measured activity. optimum was around 50 °C. A 3-fold enhancement obtained in extract...

Background: In this study, the adherence ability of Actinobacillus actinomycetemcomitans serotypes to titanium implant surfaces was evaluated demonstrate if any selective occurs according microorganism. Methods: The study material included 3 reference strains A. a, b, and c (ATCC 29523, ATCC 43718, 33384) 2 clinical isolates e (IDH 781, IDH 1705), together with commercially available blade implants. For each strain, bacterial suspensions identical concentrations (5 × 10 7 cells/ml) were...