We previously demonstrated that most eyes have regionally variable extensions of Bruch's membrane (BM) inside the clinically identified disc margin (DM) are and photographically invisible. studied impact these findings on DM- BM opening (BMO)-derived neuroretinal rim parameters.Disc stereo-photography spectral domain optical coherence tomography (SD-OCT, 24 radial B-scans centered optic nerve head) were performed 30 glaucoma patients 10 age-matched controls. Photographs colocalized to SD-OCT...

purpose. To delineate three-dimensionally the anterior and posterior surfaces of lamina cribrosa, scleral flange, peripapillary sclera, to determine position thickness these structures within digital three-dimensional (3-D) reconstructions monkey optic nerve head (ONH). methods. The trephinated ONH sclera from both eyes three monkeys with early glaucoma (EG; one eye normal, given laser-induced EG) were serially sectioned at 3-μm thickness, embedded tissue block's face stained imaged after...

To investigate whether longitudinal changes deep within the optic nerve head (ONH) are detectable by spectral domain optical coherence tomography (SDOCT) in experimental glaucoma (EG) and these at onset of Heidelberg Retina Tomography (HRT; Engineering, Heidelberg, Germany)-defined surface topography depression.Longitudinal SDOCT imaging (Spectralis; Engineering) was performed both eyes nine rhesus macaques every 1 to 3 weeks. One eye each underwent trabecular laser-induced IOP elevation....

To quantify the lamina cribrosa insertion into peripapillary sclera and optic nerve pia in normal (N) early experimental glaucoma (EEG) monkey eyes.Perfusion-fixed heads (ONHs) from 21 animals were digitally reconstructed three dimensionally delineated. Anterior Laminar Insertion Position (ALIP), Posterior (PLIP), Length (LIL; distance between anterior posterior laminar insertions), Scleral Thickness (at Sub-arachnoid space) calculated for each ONH. Animals pooled four groups based on kill...

To delineate three dimensionally the neural canal landmarks-Bruch's membrane opening (BMO), anterior sclera (ASCO), laminar insertion (ALI), posterior (PLI), and scleral (PSCO)-and anterior-most aspect of subarachnoid space (ASAS), within digital three-dimensional (3-D) reconstructions monkey optic nerve head (ONH).The trephinated ONH peripapillary from both eyes early glaucoma (EG) monkeys (one eye normal, one with laser-induced EG) were serial sectioned at 3-microm thickness, embedded...

To introduce a three-dimensional (3-D) histomorphometric strategy for characterizing the connective tissue (laminar) and prelaminar neural (prelaminar) components of optic nerve head (ONH) cupping in one bilaterally normal monkey three monkeys with early experimental glaucoma (EG) eye.Trephined ONH peripapillary sclera from both eyes four were serially sectioned at either 3-mum thickness (three EG monkeys) or 1.5-microm (the monkey) embedded block face stained imaged after each cut. Digital...

To study the anterior-posterior lamina cribrosa deformation (LCD) and scleral canal expansion (SCE) produced by an increase in IOP identify main factors interactions that determine these responses monkey.

To investigate spectral domain optical coherence tomography (SD-OCT)-detected optic disc margin anatomy in the monkey eye by colocalizing photographs to SD-OCT scans acquired from same eyes.The neural canal opening (NCO) was delineated within 40 digital radial sections generated volumes 33 normal eyes (15 degrees, 290 x 768 horizontal grid pattern). Each volume colocalized its photograph matching retinal vessels each vessel outlines visible en face images. Border tissue where it extended...

Purpose.: To determine whether acutely elevated IOP alters optic nerve head (ONH) structural parameters characterized in vivo using spectral domain optical coherence tomography (SD-OCT). Methods.: Five rhesus macaques were tested under isoflurane anesthesia. SD-OCT images of the ONH both eyes acquired 30 minutes after was stabilized to 10 mm Hg and 60 stabilization 45 Hg. The internal limiting membrane, Bruch's membrane/retinal pigment epithelium, neural canal opening (NCO), anterior lamina...

Purpose.: To retest the hypothesis that monkey ONH connective tissues become hypercompliant in early experimental glaucoma (EEG), by using 3-D histomorphometric reconstructions, and to expand characterization of EEG tissue deformation nine eyes. Methods.: Trephinated peripapillary sclera from both eyes monkeys were perfusion fixed, with one normal eye at IOP 10 mm Hg other (n = 3), 30 or 45 3) serial sectioned, reconstructed, delineated, quantified reconstruction techniques developed prior...

Purpose.: To study the association between intraocular pressure (IOP)–induced anterior–posterior lamina cribrosa deformation (LCD) and scleral canal expansion (SCE). Methods.: 3D eye-specific models of sclera eyes three normal monkeys were constructed. Morphing techniques used to produce 768 with controlled variations in geometry materials. Finite element analysis was predict LCD SCE resulting from an increase IOP. We analyzed for population as a whole, subsets. Results.: For some...

Purpose.: To characterize optic nerve head (ONH) connective tissue deformation after acute (15 or 30 minutes) intraocular pressure (IOP) elevation in six adult normal monkeys using three-dimensional (3-D) histomorphometry. Methods.: Trephined ONH and peripapillary sclera from both eyes of monkeys, each perfusion fixed with one eye at IOP 10 mm Hg (IOP-10) the other 45 (IOP-30 IOP-45, by anterior chamber manometer), were serially sectioned, 3-D reconstructed, delineated, quantified according...

purpose. To assess the ability to detect neural canal opening (NCO) and its characteristics within three-dimensional (3-D) histomorphometric 3-D spectral domain optical coherence tomography (SD-OCT) reconstructions of optic nerve head from nonhuman primate (NHP) eyes. methods. NCO was delineated 40 radial, sagittal sections 44 normal eyes 38 NHPs, each perfusion fixed at IOP 10 mm Hg, SD-OCT (Spectralis; Heidelberg Engineering, Heidelberg, Germany) volumes acquired in vivo a separate group...

We determined if the detection of spectral-domain optical coherence tomography (SDOCT) optic nerve head (ONH) change precedes confocal scanning laser (CSLT) ONH surface, SDOCT retinal fiber layer (RNFL), perimetry (SLP), and multifocal electroretinography (mfERG) in eight experimental glaucoma (EG) eyes.Both eyes from monkeys were tested at least three times baseline, then every 2 weeks following laser-induced chronic unilateral IOP elevation. Event trend-based definitions onset control EG...

To assess the effect of age on spectral-domain optical coherence tomography (SDOCT)-detected lamina cribrosa depth while controlling for visual field (VF) status and retinal nerve fiber layer thickness (RNFLT) in 221 high-risk ocular hypertension glaucoma patients enrolled Portland Progression Project.In this cross-sectional study, each participant underwent 870-nm SDOCT to obtain high-resolution radial B-scans centered optic head (ONH) a standardized ophthalmologic examination, including...

Purpose: Minimum rim width (MRW) and area (MRA) have been introduced as anatomically defensible measures of neuroretinal tissue observable using spectral-domain optical coherence tomography (SDOCT). They reported to change earlier than retinal nerve fiber layer thickness (RNFLT) in glaucoma. This study sought determine which is better distinguish subsequent from variability, the previously described longitudinal signal-to-noise ratio (LSNR). Methods: Data 157 eyes participants with high-risk...

purpose. To determine whether acutely elevated intraocular pressure (IOP) alters peripapillary retinal thickness, nerve fiber layer thickness (RNFLT), or retardance. methods. Nine adult nonhuman primates were studied while under isoflurane anesthesia. Retinal and RNFLTs measured by spectral domain optical coherence tomography 30 minutes after IOP was set to 10 mm Hg 60 45 Hg. RNFL retardance scanning laser polarimetry in 10-minute intervals for Hg, then another returned results. RNFLT 1120...

To investigate the anatomic basis of optic disc margin in normal monkey eye by colocalizing photographs to three-dimensional (3D) histomorphometric reconstructions same nerve head.Optic from 28 eyes were overlaid onto 3D central retinal vessel generated as part postmortem for each eye. Within reconstruction, Bruch's membrane opening (BMO) was delineated. Alignment achieved matching clinical outline reconstruction with parallel viewing software. An experienced observer viewed...

Purpose To quantify the effects of using fovea to Bruch's membrane opening (FoBMO) axis as nasal-temporal midline for 30° sectoral (clock-hour) spectral domain optical coherence tomography (SDOCT) optic nerve head (ONH) minimum rim width (MRW) and area (MRA) calculations. Methods The internal limiting BMO were delineated within 24 radial ONH B-scans in 222 eyes participants with ocular hypertension glaucoma. For each eye was marked infrared reflectance image, FoBMO angle (θ) relative...

Purpose: This study quantified the thickness and depth of lamina cribrosa (LC) peripapillary scleral in high-resolution three-dimensional (3D) fluorescent reconstructions optic nerve head (ONH) eyes from donors African (AD) European descent (ED). Methods: A total 64 (45 ED, 19 AD) 51 normal were obtained within 6 hours death fixed at 10 mm Hg pressure. The was trephined globe digitally reconstructed 1.5 × μm voxel resolution with an automated episcopic fluorescence technique. load-bearing...