To evaluate the efficacy of a functional microarray microneedles (MNs) plus topical tranexamic acid (TA) for melasma in middle-aged women China. Thirty female subjects with were enrolled this study. The left or right side face was chosen randomly to be pretreated MNs, followed by 0.5% TA solution once per week 12 weeks. other half-face control, treated sham device solution. At baseline and at weeks 4, 8, treatment, clinical (photographic) evaluations parameters determined Visia recorded. 12,...

China has the highest number of new cancer cases and deaths globally. Due to particularly low scores in health care quality for cutaneous squamous cell carcinoma (cSCC), country's cSCC burden requires greater awareness. Consequently, we aimed evaluate predict trend globally from 1990 2030.

Reticulate pigmented anomaly of the flexures (RPAF), also called Dowling-Degos disease, is a rare autosomal-dominant cutaneous disorder characterized by spotted and reticulate pigmentation flexures. The gene, or even chromosomal location, for RPAF has not yet been identified. In this study, one Chinese family with was identified subjected to genomewide screen linkage analysis. We locus at chromosome 17p13.3 maximum two-point limit detection score 3.61 markers D17S831and D17S1866 (at...

To investigate the diagnostic value of dermoscopy in defining tumor margin basal cell carcinoma (BCC) for appropriate surgical margin. A total 107 BCC patients were enrolled this study. The boundaries observed by naked eye and dermoscope respectively, 5 mm outward was used as according to dermoscopy-defined Pathological examinations performed at 2 intervals direction previously marked effect assessed accordingly. There still 16.8% whose visual insufficient dermoscopy-detected With excision...

Background Tacrolimus is a conventional medication for the treatment of vitiligo, but effect single limited. Objective This paper aims at observing effects, adverse responses, and repigmentation results joint vitiligo by Carbon dioxide (CO 2 ) fractional laser together with tacrolimus. Methods Forty‐five patients were randomly divided into two groups: (T) group control (C) group, each was further three subgroups (face, torso limbs, hand foot) according to location skin defect. Both groups...

We tested the susceptibility of nucleic acid strands in a halted transcription elongation complex to digestion by micrococcal nuclease (MN). The 16-nucleotide nascent RNA was protected within polymerase. A 27-28-nucleotide template strand DNA fragment also resistant MN digestion. However, upstream half nontemplate this region digested rapidly MN, suggesting that emerges from polymerase near middle melted bubble with bases oriented away enzyme surface. cleavage exposed shifted backward,...

Background: The pathogenesis of acquired melanocytic nevi (AMN) is still unclear, and the origin nevus cells has not been clarified. Objective: To analyze clinical features pathological types AMN identify possible cells. Methods: A retrospective study 2929 cases was conducted, 96 specimens intradermal junctional were selected. Immunohistochemical assays performed to detect expression basement membrane component receptor DDR-1 molecular markers on epidermal melanocytes, dermal stem (DSCs),...

Objective To investigate the diagnostic value of dermoscopy in defining tumor margin cutaneous squamous cell carcinoma (cSCC) for appropriate surgical margin. Methods A total 90 cSCC patients were enrolled study. All recruited into two groups: those who preserved intact macroscopic features neoplasms without or after incisional biopsy and with uncertain residual tumors excisional biopsy. dermoscopy-defined 8mm outward was used according to boundaries observed naked eye dermoscopy. excised...

We describe a modified method for establishing long-term pure cultures of amelanotic melanocytes (AMMC) derived from human hair follicles. Normal corpse scalp (just after death, 1 h) was transected mm below the epidermis, and follicles in remaining dermis were isolated by two-step enzyme treatment. Hair follicle cell suspensions prepared 0.50% trypsin treatment 30 min cultured an optimized melanoblast proliferation nature mitogen medium. Cells attached to substratum mostly melanocytic...

BackgroundNail matrix histopathologic examination is still the criterion standard to diagnose longitudinal melanonychia (LM).ObjectiveTo introduce modified shave surgery combined with nail window technique for managing LM and evaluate postoperative outcome of procedure.MethodsWe retrospectively reviewed medical records 67 patients who underwent longitudinal-strip at our institution from March 2015 June 2018.ResultsPathologic diagnosis was accessible in all cases, 60 cases were assessable...

In conjunction with matrix proteins, stem cell factor (SCF) plays an important role in the migration of melanocyte precursors (MPs) derived from mouse embryo. However, no studies have demonstrated effect SCF on human follicular MPs vitro. this report, first we demonstrate immature state MPs. Then attachment rate was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Standard 48-well chemotaxis chambers were used for a transfilter assay. F-actin labeled...

The melanocytes in vitiligo repigmentation are derived predominantly from melanocyte precursors (MPs) present the outer root sheath (ORS) of hair follicles. methods currently used for culturing MPs unstable, and cultured cells have capacity to produce melanin. These factors problematic when conducting vitro studies investigate mechanism repigmentation. Although 1,25-dihydroxyvitamin D3 (VID) has been demonstrated be highly effective treatment clinic, its precise mode action yet elucidated....

Objective Small intestinal neuroendocrine tumors (SI-NETs) are often detected after they have become metastatic. Using a novel protein array, we identified pathways important in SI-NET metastasis development surgically resected patients. Methods Paired primary and liver metastases from 25 patients undergoing surgical resection for metastatic SI-NETs were harvested. Extracted proteins separated by sodium dodecyl sulfate gel multiplex immunoblots performed with 136 antibodies. Significant...

Syphilis is a sexually transmitted disease caused by Treponema pallidum. The signs and symptoms of syphilis vary depending on which the four stages it presents. primary stage classically presents with painless ulcer (chancre). We report case extragenital chancre nipple examined from skin biopsy immunohistochemistry. This showed that important to identify special site's pruritus erythema pathology serological examination.

Onychopapilloma is generally recognized as a benign tumor of the nail bed and distal matrix. However, origin onychopapilloma has not been explained yet. To clarify onychopapilloma, we detected expression patterns hair-related keratins epithelial keratins, which are expressed specifically in unit. The clinical histopathologic features 11 patients with were analyzed, detected. Histologically, all subjects showed acanthosis, papillomatosis matrix metaplasia within bed. Immunohistochemically,...