A5091692161- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Acute Lymphoblastic Leukemia research
- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Mosquito-borne diseases and control
- Endoplasmic Reticulum Stress and Disease
- Paraoxonase enzyme and polymorphisms
- Viral Infections and Vectors
- Phagocytosis and Immune Regulation
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- Vector-borne infectious diseases
- Identification and Quantification in Food
- Galectins and Cancer Biology
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Listeria monocytogenes in Food Safety
- Microbial infections and disease research
- IL-33, ST2, and ILC Pathways
- Viral gastroenteritis research and epidemiology
- Bacillus and Francisella bacterial research
- Fibroblast Growth Factor Research
- Diabetes and associated disorders
Qiqihar Medical University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Soochow University
2011-2022
University of California, San Francisco
2017-2021
University of California System
2018
Beckman Research Institute
2017
City Of Hope National Medical Center
2017
City of Hope
2017
Pediatrics and Genetics
2009-2014
The University of Texas Medical Branch at Galveston
2004-2011
Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present complete genome sequence R. typhi (1,111,496 bp) compare it to two published rickettsial sequences: prowazekii conorii. We identified 877 genes in encoding 3 rRNAs, 33 tRNAs, noncoding RNAs, 838 proteins, which are frameshifts. In addition, discovered more than 40 pseudogenes, including entire cytochrome c oxidase...
Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are leading causes of upper lower tract infections in young children among elderly immunocompromised patients. The pathogenesis hMPV-induced lung disease is poorly understood. macrophage population consists alveolar macrophages (AMs) residing at the luminal surface alveoli interstitial present within parenchymal interstitium. involvement AMs innate immune responses to remains elusive. In this study, BALB/c mice depleted by...
O'nyong-nyong virus (ONNV) and chikungunya (CHIKV) are closely related alphaviruses that cause human disease in Africa Asia. Like most alphaviruses, CHIKV is vectored by culicine mosquitoes. ONNV considered unusual as it primarily infects anopheline mosquitoes; however, there relatively few experimental data to support this. In this study, three strains of one strain were evaluated Anopheles gambiae Aedes aegypti mosquitoes four cell lines. As predicted, was not infectious An. gambiae, we...
Abstract Lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hMPV) and syncytial virus (RSV) are characterized short-lasting virus-specific immunity often long-term airway morbidity, both of which may be result alterations in Ag-presenting function lung follow these infections. In this study, we investigated whether hMPV RSV experimental alter phenotype dendritic cell (DC) subsets that recruited to lung. Characterization DC trafficking demonstrated a...
Abstract Human metapneumovirus (hMPV) is a major cause of upper and lower respiratory infections in children adults. Recent work from our group demonstrated that hMPV G glycoprotein an important virulence factor, responsible for inhibiting innate immune responses airway epithelial cells. Myeloid dendritic cells (DCs) are potent APCs play role initiating modulating the adaptive responses. In this study, we found TLR4 plays hMPV-induced activation monocyte-derived DCs (moDCs), as...
Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infection in infants, young children, and high-risk adults. Currently, there no vaccine to prevent RSV infection, the available therapeutic agents are limited utility. Peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs) class antisense that can enter cells readily interfere with viral protein expression through steric blocking complementary RNA. Two PPMOs, designed target sequence includes...
Abstract Although calreticulin (CRT) is a major Ca2+-binding luminal resident protein, it can also appear on the surface of various types cells and functions as an immunopotentiating molecule. However, molecular mechanisms underlying potent immunobiological activity cell CRT are still unclear. In present study, recombinant fragment (rCRT/39–272) covering lectin-like N domain partial P murine has been expressed in Escherichia coli. The affinity-purified rCRT/39–272 assembles into homodimers...
The alphaviruses o'nyong nyong virus (ONNV) and chikungunya (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline culicine mosquitoes, whereas CHIKV only mosquitoes. In this study, chimeric viruses were constructed that contained from CHIKV. These chimeras previously described full-length infectious clones of evaluated Anopheles gambiae Aedes aegypti Virus derived retained specificity parental viruses. All six found infect Ae....
Francisella tularensis is the causative agent of tularaemia. Effective antibiotic treatment tularaemia now available, but early diagnosis remains a problem. Four primers (three pairs) were designed to detect F. by polymerase chain reaction (PCR), based on previously published nucleotide sequence T-cell epitopes membrane protein. Amplification purified chromosomal DNA with three pairs resulted in different products sizes consistent those predicted from data (211 bp, 347 bp and 568 bp). The...
We have recently reported that calreticulin (CRT), a luminal resident protein, can be found in the sera of patients with rheumatoid arthritis and also recombinant CRT (rCRT) exhibits extraordinarily strong immunological activities. herein further demonstrate rCRT fragments 18–412 (rCRT/18-412), rCRT/39-272, rCRT/120-308 rCRT/120-250 self-oligomerize solution are 50–100 fold more potent than native (nCRT, isolated from mouse livers) activating macrophages vitro. narrowed down active site to...
Significance B lymphoid transcription factors (e.g., IKZF1 and PAX5) not only mediate cell lineage commitment but also repress glucose transport energy supply, thus acting as metabolic gatekeepers. While previous studies showed regulation of gatekeeper functions at the transcriptional level, we here discovered a mechanism based on protein–protein interactions between PON2 glucose-transport inhibitor stomatin (STOM).
ABSTRACT Fragment 450–650 of the spike (S) protein (S450–650) severe acute respiratory syndrome‐associated coronavirus (SARS‐CoV) contains epitopes capable being recognized by convalescent sera SARS patients. Vaccination mice with recombinant S450–650 (rS450–650) can induce Abs against SARS‐CoV, although titer is relatively low. In present study, a fusion linking fragment (residues 39–272) murine calreticulin (CRT) to in prokaryotic expression system was created. Compared target antigen...
We have recently demonstrated that the greatly increased immunological activities of recombinant murine calreticulin (rCRT) are largely attributed to its self-oligomerization. Although native CRT (nCRT) can also oligomerize under stress conditions in vitro, whether this phenomenon could occur inside cells and activity difference between nCRT monomers oligomers remained unclear. In study, we illustrated formation tranfectant "heat & low pH" (42°C/pH 6.5) condition. The mixture (OnCRT) was...
Lactoferrin (LTF), a multifunctional glycoprotein of the transferrin family mainly found in exotic secretions mammals, is an important defense molecule against not only microbial invasion but also tumors. It folds into two globular domains (N- and C-lobes) each containing iron-binding site. The cationic antimicrobial peptide N-lobe known to exert anti-tumor effect via non-receptor-mediated pathway. However, whether LTF C-lobe contributes its activity remains be investigated. In this study,...
Clinical data reveal that patients with allogeneic hematopoietic stem cell transplantation (HSCT) are vulnerable to infection and prone developing severe sepsis, which greatly compromises the success of transplantation, indicating a dysregulation inflammatory immune response in this clinical setting. Here, by using mouse model haploidentical bone marrow (haplo-BMT), we found uncontrolled macrophage inflammation underlies pathogenesis both LPS- E.coli-induced sepsis recipient animals...
Although caltreticulin (CRT) is mainly a residential ER protein, it also expressed on the membrane surface of various types cells exhibiting multiple functions. We report here that intraperitoneal administration soluble recombinant CRT fragment (rCRT/39-272) led to substantial decrease in delayed type hypersensitivity (DTH) responses BALB/c mice and EAE C57BL/6 mice. In recall response against keyhole limpet hemocyanin (KLH) vitro, draining lymph node from rCRT/39-272-treated produced less...