A5061542116- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Bone Metabolism and Diseases
- Immune cells in cancer
- Bone health and treatments
- interferon and immune responses
- Influenza Virus Research Studies
- Polymer Nanocomposites and Properties
- Immunodeficiency and Autoimmune Disorders
- NF-κB Signaling Pathways
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Inflammatory mediators and NSAID effects
- Phagocytosis and Immune Regulation
- Respiratory viral infections research
- biodegradable polymer synthesis and properties
- Cancer Immunotherapy and Biomarkers
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Virus-based gene therapy research
- Fiber-reinforced polymer composites
- Galectins and Cancer Biology
- Endoplasmic Reticulum Stress and Disease
- Osteoarthritis Treatment and Mechanisms
Qingdao University of Science and Technology
2023-2025
Huashan Hospital
2025
Fudan University
2025
Wuhan Institute of Bioengineering
2023-2024
Soochow University
2013-2024
China National Biotechnology (China)
2023-2024
Union Hospital
2024
Huazhong University of Science and Technology
2024
Bengbu Medical College
2018-2020
Highlights•STING-induced PD-L1hi Tu.Mons dominate the resistance to STING agonist therapy•Cell-intrinsic PD-L1 induced by STING-IFN-I axis drives protumoral Tu.Mons•TLR2 activation rewires signaling reduce cell-intrinsic PD-L1•STING combined with TLR2 pre-activation shows enhanced antitumor efficacySummarySTING is an important DNA sensing machinery in initiating immune response, yet therapies targeting have shown poor outcomes clinical trials. Here, we reveal that induces tumor monocytes...
Seasonal influenza, causes hundreds of thousands deaths annually, posing a severe threat to human health. Currently available influenza vaccines are targeted only at specific strains or conserved epitopes; however, these not completely efficacious because viruses can undergo mutation during circulation, leading antigenic mismatch between recommended and circulating elusion from the immune system. Therefore, developing an vaccine that is quick, effective, broadly protective has become...
Tofacitinib (CP-690550), an oral Janus kinase inhibitor, has shown significant efficacy in the treatment of rheumatoid arthritis through blocking signaling pathways pro-inflammatory cytokines. However, recent evidence suggests that long-term tofacitinib is associated with increased risk infection (e.g. tuberculosis) patients. In present study, we illustrate administration significantly reduced survival rate mice given lethal or sub-lethal dose challenge Candida albicans. This was related to...
Vaccination remains the best prevention strategy against influenza. The MDCK-based influenza vaccine prompted development of innovative cell culture manufacturing processes. In present study, we report effects multiple administrations a candidate, seasonal, MDCK-based, quadrivalent split virus MDCK-QIV in Sprague-Dawley (SD) rats. Moreover, were evaluated terms fertility and early embryonic development, embryo-fetal perinatal toxicity SD rats immunogenicity Wistar BALB/c mice. Regarding...
Abstract Prevalence of circulating immunocomplexes (ICs) strongly correlates with rheumatoid arthritis (RA) in humans. Deposits IgG-ICs are abundant affected joints patients, yet molecular mechanisms for the pathogenic roles such ICs not fully understood. In this study, we present evidence that precipitated from RA sera sensitized human monocytes a long-lasting inflammatory functional state, characterized by strong TNF-α response to cellular proteins representing damage-associated patterns...
Abstract Cellulose nanofiber (CNF) is an advanced bio‐nanomaterial. However, the hydroxyl groups on surface of CNFs are highly polar, leading to its weak interfacial compatibility with natural rubber (NR). Therefore, it necessary organically modify by silanization improve between and NR, which can then be used prepare high‐performance CNF/NR composites. In this study, effect plasma‐activated pre‐silanization vulcanization characteristics, mechanical properties, Payne effect, dynamic...
BACKGROUND Immunotherapy presents both promises and challenges in treating hepatocellular carcinoma (HCC) due to its complex immunological microenvironment. The role of B cells, a key part the immune system, remains uncertain HCC. AIM To identify B-cell-specific signatures reveal novel immunophenotyping therapeutic targets for METHODS Using Tumor Immune Single-cell Hub 2 database, we identified B-cell-related genes (BRGs) Gene enrichment analysis was performed explore possible collaboration...
We have recently reported that calreticulin (CRT), a luminal resident protein, can be found in the sera of patients with rheumatoid arthritis and also recombinant CRT (rCRT) exhibits extraordinarily strong immunological activities. herein further demonstrate rCRT fragments 18–412 (rCRT/18-412), rCRT/39-272, rCRT/120-308 rCRT/120-250 self-oligomerize solution are 50–100 fold more potent than native (nCRT, isolated from mouse livers) activating macrophages vitro. narrowed down active site to...
Abstract In this paper, an accelerant with vulcanization‐promoting and silanization‐promoting effects, 1,3‐diphenylguanidine (DPG), is loaded on green filler silica by high‐energy ball milling technology. Meanwhile, DPG breaks the ionization equilibrium of latex realizes self‐flocculation. The DPG‐induced self‐flocculation technology promotes dispersion silanization reaction, forming more complete filler‐rubber network three‐dimensional crosslinking network. X‐ray diffraction,...
Poor immune responses to inactivated influenza vaccine can be improved by effective and safe adjuvants increase antibody titers cellular protective response. In our study, AddaVax PolyI:C combined adjuvant (AP adjuvant) were used for development. After immunizing BALB/c mice Wistar rats intramuscularly, Split H3N2 adjuvanted with AP elicited higher serum hemagglutination-inhibition antibodies IgG titers. We demonstrated that induced a transient innate cytokines production at the injection...
Given the importance of B lymphocytes in inflammation and immune defense against pathogens, mice transgenic for Cre under control Cd19 promoter (Cd19Cre/+ mice) have been widely used to specifically investigate role loxP-flanked genes cell development/function. However, impacts expression/insertion transgene on phenotype function cells not carefully studied. Here, we show that number marginal zone B1a was selectively reduced Cd19Cre/+ mice, while development bone marrow total numbers...
Abstract In this study, a VOC‐free animal glycerol potassium salt is introduced to replace the silane coupling agent, and plasma used treat modifier silica. The impact of high‐energy electrons in increases contact area between Combined with atomization technology, velocity difference realizes microscopic dispersion silica, instantaneous thin‐layer drying reduces oxidation rubber chains. XPS SEM show that promotes silanization reaction FTIR demonstrates existence chemical bonds hydrogen...
Rheumatoid arthritis (RA) is a chronic autoimmune disease accompanied by local and systemic bone loss. FcγRs, especially FcγRIIa (hFcγRIIa), have been implicated in the pathogenesis of RA. However, contribution hFcγRIIa to loss has not fully elucidated. In present study, we demonstrated double-edged sword role on osteoclast differentiation through investigations involving hFcγRIIa-transgenic (hFcγRIIa-Tg) mice. Our findings reveal that hFcγRIIa-Tg mice, previously shown exhibit heightened...
Calreticulin (CRT), a multifunctional Ca2+-binding glycoprotein mainly located in the endoplasmic reticulum, is tumor-associated antigen that has been shown to play protective roles angiogenesis suppression and anti-tumor immunity. We previously reported soluble CRT (sCRT) was functionally similar heat shock proteins or damage-associated molecular patterns terms of ability activate myeloid cells elicit strong inflammatory cytokine productions. In present study, B16 melanoma cell lines...
Tumor necrosis factor receptor-associated 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) resistance to influenza A virus (IVA) remains elusive. In the present study, CRISPR-Cas9 gene editing technology was used construct knockout MDCK cells (MDCK-TRAF3−/−). Hemagglutination assay, plaque transcriptome, quantitative real-time PCR were performed after IVA infection. results...
An endoplasmic reticulum resident protein, calreticulin (CRT), participates in many cellular processes. CRT is a tumor-associated antigen with an important role antitumor immunity. Previously, we reported that the recombinant fragment 39-272 (CRT/39-272) exhibited superior immunobiological activity, activating macrophages to release cytokines and promoting dendritic cell (DC) maturation. However, effect of CRT/39-272 vivo, especially its adjuvant on vivo immune responses, was not fully...
1. This study determined the effects of (E)-3-(2-(4-(3-(2,4-dimethoxyphenyl)acryloyl)phenoxy)ethoxy)-5,7-dimethoxy-2-(3,4,5-trimethoxyphenyl)-4H-chromen-4-one (EDACO) on differentiation Gaoyou duck embryonic osteoclasts cultured in vitro. 2. Bone marrow mononuclear cells (BM-MNC) were collected from 23-d-old embryos and induced by macrophage colony-stimulating factor receptor activator nuclear κB ligand presence EDACO at different concentrations (i.e. 10, 20, 40, 80 160 µM)....
ABSTRACT Potentiation of receptor activator NF-κB ligand (RANKL)-induced osteoclastogenesis by IgG immunocomplexes (ICs) is generally considered an important pathway leading to cartilage and bone destruction in rheumatoid arthritis (RA). However, whether ICs possess pro-osteoclastogenic potential independent RANKL inflammatory cytokines unclear. Here we demonstrate that fully cross-linking human FcγRIIa (hFcγRIIa) or co-ligating hFcγRIIa TLR4, alone could drive the differentiation blood...
Much evidence suggests that trained immunity is inappropriately activated in the synovial tissue rheumatoid arthritis (RA), but underlying mechanism remains unclear. Here, we describe how RA-specific autoantibody deposits can train human monocytes to exert hyperactive inflammatory response, particularly via exacerbated release of tumor necrosis factor α (TNFα). Comparative transcriptomic analysis by plate-bound IgG (cIgG) or β-glucan indicated metabolic shift towards glycolysis a crucial for...
Calreticulin (CRT), a luminal resident calcium-binding glycoprotein of the cell, is tumor-associated antigen involved in tumorigenesis and also an autoantigen targeted by autoantibodies found patients with various autoimmune diseases. We have previously shown that prokaryotically expressed recombinant murine CRT (rCRT) exhibits strong stimulatory activities against monocytes/macrophages vitro potent immunogenicity vivo, which partially attributable to self-oligomerization soluble rCRT....