A5103016130- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Biosensors and Analytical Detection
- Infectious Diseases and Mycology
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Esophageal Cancer Research and Treatment
- Renal Transplantation Outcomes and Treatments
- Infectious Diseases and Tuberculosis
- Organ Transplantation Techniques and Outcomes
- Oral microbiology and periodontitis research
- Advanced biosensing and bioanalysis techniques
- Eosinophilic Esophagitis
- Prostate Cancer Treatment and Research
- Advanced Biosensing Techniques and Applications
- Synthesis of β-Lactam Compounds
- Pharmacy and Medical Practices
- Syphilis Diagnosis and Treatment
- Liver Diseases and Immunity
- Escherichia coli research studies
Colorado State University
2011-2024
University of Indonesia
2024
University of Colorado Denver
2024
National Jewish Health
2024
University of North Carolina at Chapel Hill
2019
ETH Zurich
2010
Beaumont Hospital, Troy
2010
Queen Mary University of London
2008
Baylor College of Medicine
2006-2007
Biotechnology Institute
2004
We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis T. reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive cooperative measures, consistent its pathogenic nature lifestyle within mixed-species environment subgingival dental plaque. Comparisons previously sequenced genomes revealed specific contributing to differences similarities in physiology as...
Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present complete genome sequence R. typhi (1,111,496 bp) compare it to two published rickettsial sequences: prowazekii conorii. We identified 877 genes in encoding 3 rRNAs, 33 tRNAs, noncoding RNAs, 838 proteins, which are frameshifts. In addition, discovered more than 40 pseudogenes, including entire cytochrome c oxidase...
A crucial and distinctive feature of tuberculosis infection is that Mycobacterium (Mtb) resides in granulomatous lesion at various stages disease development necrosis, an aspect little understood. We used a novel approach, applying high resolution magic angle spinning nuclear magnetic resonance spectroscopy (HRMAS NMR) directly to infected tissues, allowing us study the granulomas guinea pigs untargeted manner. Significant up-regulation lactate, alanine, acetate, glutamate, oxidized reduced...
ABSTRACT Ethambutol (EMB) is an antimycobacterial drug used extensively for the treatment of tuberculosis caused by Mycobacterium . EMB targets biosynthesis cell wall, inhibiting synthesis both arabinogalactan and lipoarabinomannan (LAM), assumed to act via inhibition three arabinosyltransferases: EmbA, EmbB, EmbC. EmbA EmbB are required arabinogalactan, at least one enzyme ( M. [EmbA Mt ]) essential in EmbC also viability but involved LAM. We show that mutations reduce its...
The Emb proteins (EmbA, EmbB, EmbC) are mycobacterial arabinosyltransferases involved in the biogenesis of cell wall. EmbA and EmbB predicted to work unison as a heterodimer. formation crucial terminal hexaarabinoside motif [Araβ(1→2)Araα(1→5)] [Araβ(1→2)Araα(1→3)]Araα(1→5)Araα1→(Ara6) wall polysaccharide arabinogalactan. Studies conducted Mycobacterium smegmatis revealed that mutants with disruptions embA or embB viable, although growth rate was affected. In contrast, we demonstrate here is...
With the understanding that laboratory propagated strain of Mycobacterium tuberculosis H37Rv is modest virulence and drug susceptible, in present study, we performed a nuclear magnetic resonance-based metabolomic analysis lung tissues serum obtained from guinea pigs infected by low dose aerosol exposure to clinical isolates tuberculosis. High Resolution Magic Angle Spinning NMR coupled with multivariate statistical 159 multiple locations age-matched naïve 30 60 days pig lungs revealed wide...
Bacteriophages (phages) are viruses that selectively infect bacteria and have been utilized to treat Mycobacterium abscessus (Mab) with varying success. The POSTSTAMP study is an ongoing, multi-site phage therapy protocol for treatment-refractory pulmonary Mab disease in people cystic fibrosis (pwCF). Participants (n = 10) prospectively assessed while utilizing FDA investigational new drug (IND) approval compassionate use. >6 years old, able produce sputum, treated guideline-based antibiotic...
Structure-based design was used to develop a focused library of A-ring-modified diphenyl ether InhA inhibitors. From this analogs, two high-affinity alkyl-substituted ethers, 6PP and 8PP, were selected for advanced study into their in vitro activity against Mycobacterium tuberculosis clinical isolates, vivo properties, signature response mode action. 8PP demonstrated enhanced whole bacteria showed rapid macrophage model infection. In addition, transcriptional profiling revealed that the...
ABSTRACT Arabinan polymers are major components of the cell wall in Mycobacterium tuberculosis and involved maintaining its structure, as well playing a role host-pathogen interactions. In particular, lipoarabinomannan (LAM) has multiple immunomodulatory effects. nonpathogenic species smegmatis , EmbC been identified key arabinosyltransferase incorporation arabinose into LAM, an embC mutant is viable but lacks LAM. contrast, we demonstrate here that M. essential gene under normal growth...
Globally, tuberculosis is slowly declining each year and it estimated that 37 million lives were saved between 2000 2013 through effective diagnosis treatment. Currently, relies on demonstration of the bacteria, Mycobacterium (Mtb), in clinical specimens by serial sputum microscopy, culture molecular testing. Commercial immunoassay lateral flow kits developed to detect Mtb lipoglycan lipoarabinomannan (LAM) urine as a marker active TB exhibit poor sensitivity, especially immunocompetent...
Abstract HLA eplet mismatch load has been suggested as an improvement to antigen determination for organ selection. Given that mismatches are determined based on amino acid sequence difference among alleles, and the frequency of alleles varies between racial groups, we investigated correlation allograft outcomes in 110 pediatric kidney transplant recipients who received their first from a donor same race (SRT) versus different (DRT). Adjusted modified Poisson regression was used assess...
ABSTRACT The genome of Treponema paraluiscuniculi strain Cuniculi A was compared to the syphilis spirochete pallidum subsp. Nichols using DNA microarray hybridization, whole-genome fingerprinting, and sequencing. T. containing all 1,039 predicted open reading frame PCR products used identify deletions major sequence changes in genome. Using these approaches, deletions, insertions, prominent were found 38 gene homologs six intergenic regions when it Nichols. Most observed differences...
The World Health Organization (WHO) calls for the development of a rapid, biomarker-based, non-sputum test capable detecting all forms tuberculosis (TB) at point-of-care to enable immediate treatment initiation. Lipoarabinomannan (LAM) is only WHO-endorsed TB biomarker that can be detected in urine, an easily collected sample matrix. For obtaining optimal sensitivity, we and others have shown some form pretreatment necessary remove background from patient urine samples. A number systems are...
In Mycobacterium tuberculosis (Mtb), surface-exposed Lipoarabinomannan (LAM) is a key determinant of immunogenicity, yet its intrinsic heterogeneity confounds typical structure-function analysis. Recently, LAM gained strong foothold as validated marker for active (TB) infection and has shown great potential in new diagnostic efforts. However, no efforts have been made to model or evaluate the impact mixed polyclonal Mtb infections (infection with multiple strains) on TB procedures other than...
ELISA on microfluidic interface device for lipoarabinomannan.
Lipoarabinomannan (LAM) is a unique cell wall component containing fatty acid region with large mannan core attached to poly-arabinan chains, sometimes followed by mannose capping in Actinomyces species. The hydrophobic made of tuberculostearic (TBSA) and two palmitic chains linked phosphoinositol, anchors LAM the mycobacterial wall. As major constituent all mycobacteria, includingMycobacterium tuberculosis(M.tb),LAM has been shown be useful diagnostic biomarker identification MTB infection...
Our study sought to determine whether urine lipoarabinomannan (LAM) could be validated in a sample cohort that consisted mainly of HIV uninfected individuals presented with tuberculosis symptoms. We evaluated two tests developed our laboratory, and used them on clinical samples from Lima, Peru where incidence is low. ELISA analysis was performed 160 (from 140 adult culture-confirmed TB cases 20 symptomatic TB-negative child controls) using 100 μL after pretreatment Proteinase K. Two...
BackgroundIndividuals with Cystic fibrosis (CF) are the most vulnerable population for pulmonary infection nontuberculous mycobacteria (NTM). Screening, diagnosis, and assessment of treatment response currently depend on traditional culture techniques, but sputum analysis NTM in CF is challenging, associated a low sensitivity. The cell wall lipoarabinomannan (LAM), lipoglycan found all mycobacterial species, has been validated as biomarker urine active Mycobacterium tuberculosis...