A5043281036- Mycobacterium research and diagnosis
- Viral Infections and Vectors
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-borne infectious diseases
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Biotin and Related Studies
- Parvovirus B19 Infection Studies
- Oral Health Pathology and Treatment
- Plant Virus Research Studies
- Insect and Pesticide Research
- Viral Infections and Immunology Research
- Veterinary medicine and infectious diseases
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
Ingenasa (Spain)
1992-2021
Inmunal (Spain)
1994-2019
Centre de Recerca en Sanitat Animal
2012
Universitat Autònoma de Barcelona
2012
University of Alabama at Birmingham
1994
University of Oxford
1994
Background/Objectives: African Swine Fever (ASF) is one of the most significant infectious diseases affecting both domestic pig and wild boar populations, leading to substantial economic biosanitary consequences. In Europe, disease management relies on stringent biosecurity measures surveillance through diagnosis, highlighting urgent need for an effective safe vaccine ASF control. this context, VACDIVA project has generated several promising candidates, including those with EP153R gene...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected more than 8 million people worldwide, becoming a pandemic. Detecting antibodies against SARS-CoV-2 is of utmost importance and good indicator exposure circulation the virus within general population. Two serological tools based on double recognition assay [enzyme-linked immunosorbent (DR-ELISA) lateral flow (DR-LFA)] to detect total have been developed recombinant nucleocapsid protein. A 1065 serum samples, including...
The native Eurasian wild boar (Sus scrofa) is a reservoir of Mycobacterium bovis, the causative agent animal tuberculosis (TB), chronic disease in livestock, companion animals and mammals. Cases M. bovis infection or feral pig have been reported worldwide, making early detection priority eradication disease. Point-of-care diagnostic tests, such as low cost lateral flow assays, provide high specificity sensitivity can be performed on site, an essential requirement for rapid screening...
Early diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV) is critically important for control the disease. Two new commercially available enzyme-linked immunosorbent assays (ELISAs) based on different methodologies have been developed. In present report, 2 ELISAs were compared using blood samples from experimentally naturally infected pigs. One was shown to be more sensitive than other. The higher sensitivity one could pose a problem in PRRS endemic farms, because it can...
An indirect enzyme-linked immunosorbent assay (ELISA) based on baculovirus recombinant P30 protein of Ehrlichia canis and the 1BH4 anticanine IgG monoclonal antibody was developed evaluated by examining a panel 98 positive 157 negative sera using fluorescent (IFA) test as reference technique. The P30-based ELISA appeared to be sensitive specific (77.55% 95.54%, respectively) when qualitative results (positive/negative) were compared with those IFA test; coefficient correlation (R) between 2...
A new commercially available antigen-capture, blocking enzyme-linked immunosorbent assay (antigen-capture b-ELISA), based on baculovirus truncated-S recombinant protein of Transmissible gastroenteritis virus (TGEV) and 3 specific monoclonal antibodies, was developed evaluated by examining a panel 453 positive Porcine respiratory coronavirus (PRCoV), 31 TGEV, 126 negative field sera using another differential b-ELISA as the reference technique to differentiate TGEV- from PRCoV-induced...
ss Open Acce Poster Presentation Evaluation of the baculovirus and E.coli-expressed non-structural (NS) proteins bluetongue virus (BTV) as antigen in an indirect or competition ELISA to differentiate infected from vaccinated animals Lissette Lopez*1, Angel Venteo1, Miguel Jimenez-Clavero2, Juan Luis Carrasco1, Maria Jose Cano1, Elena Soria1, Concepcion GomezTejedor2, Antonio Sanz1, Carmen Vela1 Paloma Rueda1
West Nile virus (WNV) is a zoonotic arboviral pathogen affecting humans, birds, and horses. Vaccines are available for veterinary use, which efficiently prevent the infection in Most common diagnostic tools rely on identification of agent (RT-PCR, isolation), or detection antibodies (IgM IgG) recognizing structural proteins neutralizing cell cultures (virus-neutralization tests). The recent emergence WNV different parts world has resulted an increase vaccination horses many countries....
During the COVID-19 pandemic, infection of farmed mink has become not only an economic issue but also a widespread public health concern. International agencies have advised use strict molecular and serosurveillance methods for monitoring SARS-CoV2 status on farms. We developed 2 ELISAs duplex protein microarray immunoassay (MI), all in double-recognition format (DR), to detect antibodies specific receptor-binding domain (RBD) spike full-length nucleoprotein (N) sera. collected 264 serum...
A duplex rapid assay for detection of serum antibodies to canine parvovirus (CPV) was developed. Canine immunoglobulin (Ig)M or IgG were captured in immunotubes with anti-canine IgM and detected VP2 recombinant protein followed by an anti-VP2 monoclonal antibody. The tested using a collection sera from dogs that vaccinated against CPV on arrival at animal shelter Madrid, Spain. Results compared those 2 commercial enzyme-linked immunosorbent assays (ELISAs) considered as reference techniques....