A5088592256- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Plant-Microbe Interactions and Immunity
- Fungal Infections and Studies
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Antifungal resistance and susceptibility
- Mycorrhizal Fungi and Plant Interactions
- Biofuel production and bioconversion
- NF-κB Signaling Pathways
- bioluminescence and chemiluminescence research
- Cellular transport and secretion
- Yeasts and Rust Fungi Studies
- Toxin Mechanisms and Immunotoxins
- Plant Disease Resistance and Genetics
- Heat shock proteins research
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Signaling Pathways in Disease
Kyungpook National University
2017-2025
Filamentous fungi produce a vast number of asexual spores that act as efficient propagules. Due to their infectious and/or allergenic nature, fungal affect our daily life. Aspergillus species called conidia; formation involves morphological development and metabolic changes, the associated regulatory systems are coordinated by multiple transcription factors (TFs).
ABSTRACT All life forms have evolved to respond appropriately various environmental and internal cues. In the animal kingdom, prototypical regulator class of such cellular responses is Rel homology domain proteins including nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB). Fungi, close relatives animals, also with their own NF-κB-like regulators called velvet family govern chemical development. Here, we conducted a detailed investigation taxonomic broad presence proteins....
Abstract Myeloblastosis (MYB)-like proteins are a family of highly conserved transcription factors in animals, plants, and fungi involved the regulation mRNA expression genes. In this study, we identified characterized one MYB-like protein model organism Aspergillus nidulans . We screened levels genes encoding containing two MYB repeats conidia found that four including flbD , cicD uncharacterized genes, were high conidia. To investigate roles AN4618 AN10944 deletion mutants for each gene...
Abstract The homeobox domain-containing transcription factors play an important role in the growth, development, and secondary metabolism fungi other eukaryotes. In this study, we characterized roles of genes coding for homeobox-type proteins model organism Aspergillus nidulans . To examine their A. , deletion mutant strains each gene protein were generated, phenotypes examined. Phenotypic analyses revealed that two proteins, HbxA HbxB, required conidia production. Deletion hbxA caused...
Filamentous fungi reproduce asexually or sexually, and the processes of asexual sexual development are tightly regulated by a variety transcription factors. In this study, we characterized Zn2Cys6 factor in two Aspergillus species, A. nidulans (AN5859) flavus (AFLA_046870). AN5859 encodes factor, called ZcfA. nidulans, ΔzcfA mutants exhibit decreased fungal growth, reduction cleistothecia production, increased reproduction. Overexpression zcfA results conidial suggesting that ZcfA is...
Filamentous fungi produce myriads of asexual spores, which are the main reproductive particles that act as infectious or allergenic agents. Although serial sporogenesis is coordinated by various genetic regulators, there remain uncharacterized transcription factors in Aspergillus. To understand underlying mechanism spore formation, integrity, and viability, we have performed comparative transcriptomic analyses on three Aspergillus species found a spore-specific factor, SscA. SscA has major...
Abstract Calcineurin modulates environmental stress survival and virulence of the human fungal pathogen Cryptococcus neoformans. Previously, we identified 44 putative calcineurin substrates, proposed that pathway is branched to regulate targets including Crz1, Pbp1, Puf4 in C. In this study, characterized Had1, which one substrates belonging ubiquitously conserved haloacid dehalogenase β-phosphoglucomutase protein superfamily. Growth had1∆ mutant was found be compromised at 38° or higher....
The calcineurin pathway is an important signaling cascade for growth, sexual development, stress response, and pathogenicity in fungi. In this study, we investigated the function of CrzA, a key transcription factor pathway, aflatoxin-producing fungus Aspergillus flavus (A. flavus). To examine role crzA gene, deletion mutant strains A. were constructed their phenotypes, including fungal spore formation, sclerotial examined. Absence results decreased colony number conidia, sclerocia...
In the Aspergillus species, conidia are asexual spores that infectious particles responsible for propagation. Conidia contain various mycotoxins can have detrimental effects in humans. Previous study demonstrated VadA is required fungal development and spore viability model fungus nidulans. present study, vadA transcriptomic analysis revealed affects mRNA expression of a variety genes A. nidulans conidia. The were primarily affected associated with trehalose biosynthesis, cell-wall...
The Mkt1-Pbp1 complex promotes mating-type switching by regulating the translation of HO mRNA in Saccharomyces cerevisiae. Here, we performed vivo immunoprecipitation assays and mass spectrometry analyses human fungal pathogen Cryptococcus neoformans to show that Pbp1, a poly(A)-binding protein-binding protein, interacts with Mkt1 containing PIN like-domain. Association Pbp1 was confirmed co-immunoprecipitation assays. Results spot dilution growth showed unlike pbp1 deletion mutant strains,...
McrA is a key transcription factor that functions as global repressor of fungal secondary metabolism in Aspergillus species. Here, we report mcrA one the VosA-VelB target genes and governs cellular metabolic development nidulans. The deletion resulted reduced number conidia decreased mRNA levels brlA, asexual developmental activator. In addition, absence led to loss long-term viability spores (conidia), which likely associated with lack conidial trehalose increased β-(1,3)-glucan conidia....
Mon1 is a guanine nucleotide exchange factor subunit that activates the Ypt7 Rab GTPase and essential for vacuole trafficking autophagy in eukaryotic organisms. Here, we identified characterized function of Mon1, an ortholog Saccharomyces cerevisiae human fungal pathogen, Cryptococcus neoformans. Mutation mon1 resulted hypersensitivity to thermal stress. The deletion mutant exhibited increased sensitivity cell wall endoplasmic reticulum However, showed more resistance antifungal agent...
Homeobox transcription factors are conserved in eukaryotes and act as multi-functional filamentous fungi. Previously, it was demonstrated that HbxB governs fungal development spore viability Aspergillus nidulans. Here, the role of A. nidulans further characterized. RNA-sequencing revealed affects transcriptomic levels genes associated with trehalose biosynthesis response to thermal, oxidative, radiation stresses asexual spores called conidia. A phenotypic analysis found hbxB deletion mutant...
The velvet regulators VosA and VelB are primarily involved in spore maturation dormancy. Previous studies found that the VosA-VelB hetero-complex coordinates certain target genes related to fungal differentiation conidial Aspergillus nidulans. Here, we characterized VosA/VelB-inhibited developmental gene vidD A. Phenotypic analyses demonstrated deleted mutant exhibited defect growth, a reduced number of conidia, delayed formation sexual fruiting bodies. deletion decreased amount trehalose,...
MonA is a subunit of guanine nucleotide exchange factor that important for vacuole passing and autophagy processes in eukaryotes. In this study, we characterized the function MonA, an orthologue Saccharomyces cerevisiae Mon1, model fungus Aspergillus nidulans toxigenic A. flavus. nidulans, absence AnimonA led to decreased fungal growth, reduced asexual reproduction, defective cleistothecia production. addition, deletion mutants exhibited spore viability, had trehalose contents conidia, were...
Abstract A major group of fungi produces asexual spores (conidia) for propagation and infection. Despite the critical role conidia, underlying mechanism spore formation, integrity, viability is not fully elucidated. In this study, we have identified investigated spore-specific transcription factor (TF) SscA in three representative Aspergillus species. Comparative transcriptomic analyses revealed that 25 TF encoding genes showed higher mRNA levels conidia than hyphae Functional as a key...