A5033210923- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Coral and Marine Ecosystems Studies
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Chemical synthesis and alkaloids
- Cancer therapeutics and mechanisms
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Marine Invertebrate Physiology and Ecology
- Melanoma and MAPK Pathways
- Natural product bioactivities and synthesis
- Synthesis and biological activity
- Mycotoxins in Agriculture and Food
- HIV Research and Treatment
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Marine and coastal plant biology
- Protist diversity and phylogeny
- Vanadium and Halogenation Chemistry
- Synthetic Organic Chemistry Methods
University of Florida
2020-2025
Whitney Museum of American Art
2020-2024
University of St. Augustine for Health Sciences
2022
University of Mississippi
2022
Oregon State University
2014-2021
National Institute of Diabetes and Digestive and Kidney Diseases
2011-2017
University of Göttingen
2011-2014
National Institutes of Health
2011-2013
Center for Biologics Evaluation and Research
2013
United States Food and Drug Administration
2013
Meroterpenoids are mixed polyketide-terpenoid natural products with a broad range of biological activities. Herein, we present the structures four new meroterpenoid antibiotics, merochlorins A-D, produced by marine bacterium Streptomyces sp. strain CNH-189, which possess novel chemical skeletons unrelated to known bacterial agents. Draft genome sequencing, mutagenesis, and heterologous biosynthesis in genome-minimized model actinomycete coelicolor provided 57.6 kb merochlorin gene cluster...
Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism. Cancer are known to reprogram their metabolism using different strategies meet energetic anabolic needs ( Koppenol et al., 2011 ; Zheng, 2012). Additionally, each cancer tissue has its own individual metabolic features. Mitochondria not only play a key role in energy but also cell cycle regulation of cells. Therefore, mitochondria have emerged as potential target for...
Abstract To determine the best sources of novel, biologically active metabolites, both endophytic fungi (plant isolates) and associated with algae were isolated from plants marine habitats North, Baltic Mediterranean Seas, Atlantic Ocean, Gulf Mexico. Following preselection isolates according to taxon metabolic profiles, almost all in at least one tests for antibacterial, antifungal, and/or herbicidal activities. Metabolites culture extracts belonged diverse structural groups; 42% previously...
Background We evaluated the in vitro activity of a merochlorin A, novel compound with unique carbon skeleton, against spectrum clinically relevant bacterial pathogens and previously characterized clinical laboratory Staphylococcus aureus isolates resistance to numerous antibiotics. Methods Merochlorin A was isolated purified from marine-derived actinomycete strain CNH189. Susceptibility testing for performed human using broth microdilution agar dilution methods. Cytotoxicity assayed tissue...
A new bicyclic diterpenoid, benditerpenoic acid, was isolated from soil-dwelling Streptomyces sp. (CL12-4). We sequenced the bacterial genome, identified responsible biosynthetic gene cluster, verified function of terpene synthase, and heterologously produced core diterpene. Comparative bioinformatics indicated this strain is phylogenetically unique possesses nine synthases. The absolute configurations trans-fused bicyclo[8.4.0]tetradecanes were achieved by extensive spectroscopic analyses,...
Filamentous fungi produce a vast number of asexual spores that act as efficient propagules. Due to their infectious and/or allergenic nature, fungal affect our daily life. Aspergillus species called conidia; formation involves morphological development and metabolic changes, the associated regulatory systems are coordinated by multiple transcription factors (TFs).
The advent of antibiotic resistance in microorganisms requires the discovery and synthesis novel antibiotics. At same time, human pathogens are contributing to chronic persistent inflammation. Motivated by these two concerning issues, new drug candidates synthesized incorporation benzothiazine, pyrazole, amide moieties a scaffold create multifunctional derivatives pyrazolo-1,2-benzothiazine. presented compounds have been analyzed using spectroscopic spectrometric techniques including FTIR,...
Behavioral models have served a key role in understanding nociception, the sensory system by which animals detect noxious stimuli their environment. Developing zebrafish (Danio rerio) are powerful study organism for examining nociceptive pathways, given vast array of genetic, developmental, and neuroscience tools available these animals. However, at present there few widely-adopted behavioral nociception developing zebrafish. This examines locomotor response hatching-stage embryos to dilute...
Plant or microbial lectins are known to exhibit potent antiviral activities against viruses with glycosylated surface proteins, yet the mechanism(s) by which these carbohydrate-binding proteins exert their is not fully understood. Hepatitis C virus (HCV) possess envelope (gpE1E2) and be potently inhibited lectins. Here, we tested in detail properties of newly discovered Microcystis viridis lectin (MVL) along cyanovirin-N (CV-N) Galanthus nivalis agglutinin (GNA) cell culture HCV, as well...
The genetically encoded, small-molecule chemical diversity of filamentous fungi is still largely unexplored and represents an attractive source for the discovery new compounds. Here we report production chlorinated bianthrones from coculture two different developmental stages, or morphs, a marine alga-derived Aspergillus alliaceus (teleomorph: Petromyces alliaceus) strain. vegetative stage (asexual morph) can be separated morph that switched to sexual development (sclerotial morph); both...
Mutualisms between cnidarian hosts and dinoflagellate endosymbionts are foundational to coral reef ecosystems. These symbioses often re-established every generation with high specificity, but gaps remain in our understanding of the cellular mechanisms that control symbiont recognition uptake dynamics. Here, we tested whether differences glycan profiles among different species account for rates at which they initially colonize aposymbiotic polyps model sea anemone Aiptasia (Exaiptasia...
Abstract A comparative metabolomic study of a marine derived fungus ( Aspergillus terreus ) grown under various culture conditions is presented. The was in eleven different using solid agar, broth cultures, or grain based media (OSMAC). Multivariate analysis LC/MS data from the organic extracts revealed drastic differences metabolic profiles and guided our subsequent isolation efforts. compound 7‐desmethylcitreoviridin isolated identified, fully described for first time. In addition, 16...
Bioactivity-guided isolation of Aspergillus ustus led to the discovery five new drimane sesquiterpenes, named ustusal A, ustusolate F and G, ustusoic acid A B, 1–5 respectively. Structural elucidation these fungal terpenes relied on 1D 2D NMR techniques, high-resolution mass spectrometry, chiroptical properties. Their relative configurations were determined by methods, while absolute established using comparative analyses computed experimental chemical shifts ECD spectra. The sesquiterpenes...
The diversity of genetically encoded small molecules produced by filamentous fungi remains largely unexplored, which makes these an attractive source for the discovery new compounds. However, accessing their full chemical repertoire under common laboratory culture conditions is a challenge. Epigenetic manipulation gene expression has become well-established tool overcoming this obstacle. Here, we report that perturbation endophytic ascomycete Chalara sp. 6661, producer isofusidienol class...
Fusarium graminearum, the primary cause of head blight (FHB) in small-grain cereals, demonstrates remarkably variable levels aggressiveness its host, producing different infection dynamics and contrasted symptom severity. While secreted proteins, including effectors, are thought to be one essential components aggressiveness, our knowledge intra-species genomic diversity F. graminearum is still limited. In this work, we sequenced eight European strains contrasting characterize their...
Thalassia testudinum has undergone die-offs in the past century due to seagrass wasting disease caused by Labyrinthula sp. Little is known about how seagrasses resist infections, but metabolites that inhibit were previously extracted from leaves. Furthermore, leaf fungal endophytes possess antipathogenic potential, their activity against unknown. Here, we aimed identify whether of T. can aid defense . Through Illumina amplicon sequencing leaves’ mycobiome, identified fungi are produce...
The chemical logic associated with assembly of many bacterial terpenoids remains poorly understood. We focused our efforts on the early-stage biosynthesis phenalinolactone diterpenoids, demonstrating that
Abstract From the culture extracts of endophytic fungus, Phoma sp., isolated from plant Salsola oppositifolia , five secondary metabolites were and characterized. On biomalt agar medium, strain produced known substances chrysophanol ( 1 ) hypothemycin 2 ), while liquid‐surface fermentations on a glucose/maltose 2,4‐pyridione derivatives obtained. Interestingly, one compounds, flavipucine 3a showed positive [ α ] D ; it, thus, was enantiomer known, but configurationally as yet unassigned...
ABSTRACT The molecular fingerprinting technique terminal-restriction fragment length polymorphism (T-RFLP) was used in combination with sequence-based approaches to evaluate the geographic distribution of secondary metabolite biosynthetic genes strains marine actinomycete Salinispora arenicola . This study targeted ketosynthase (KS) domains from type I polyketide synthase (PKS) and revealed four distinct clusters, largest which comprised all six global locations sampled. remaining fell into...