A5089965213- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- 14-3-3 protein interactions
- Light effects on plants
- Heavy Metal Exposure and Toxicity
- Microbial Natural Products and Biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Protist diversity and phylogeny
- Heavy metals in environment
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Bacterial Identification and Susceptibility Testing
- Plant and fungal interactions
- Urinary Tract Infections Management
- Clinical practice guidelines implementation
- bioluminescence and chemiluminescence research
- Redox biology and oxidative stress
- Chromium effects and bioremediation
University of Wisconsin–Madison
2018-2023
In the filamentous fungus Aspergillus nidulans, velvet family protein VeA and global regulator of secondary metabolism LaeA govern development mostly by acting as VelB/VeA/LaeA heterotrimeric complex. While functions these highly conserved controllers have been well studied, genome-wide regulatory networks governing cellular chemical remain to be uncovered. Here, integrating transcriptomic analyses, protein-DNA interactions, known A. nidulans gene/protein interaction data, we unraveled gene...
Filamentous fungi produce a vast number of asexual spores that act as efficient propagules. Due to their infectious and/or allergenic nature, fungal affect our daily life. Aspergillus species called conidia; formation involves morphological development and metabolic changes, the associated regulatory systems are coordinated by multiple transcription factors (TFs).
Sesame Sesamum indicum L. is a major oil-based seed crop that has been widely cultivated and consumed in Pakistan. Unfortunately, sesame highly prone to Aspergillus fungal growth the field, under inappropriate storage conditions can become contaminated with aflatoxins, most potent carcinogen found nature. Here, we have isolated high number of isolates from seeds fresh stored obtained rainfed irrigated zones Punjab, Pakistan, characterized them for aflatoxigenic potentials. Using...
Cadmium is an exceptionally toxic industrial and environmental pollutant classified as a human carcinogen. In order to provide insight into how we can keep our environment safe from cadmium contamination prevent the accumulation of it in food chain, aim elucidate Aspergillus nidulans, one most abundant fungi soil, survives handles stress. As AtfA main transcription factor governing stress responses A. examined genome-wide expression wild-type atfA null mutant exposed CdCl2. Both strains...
Glutathione (GSH) is an abundant tripeptide that plays a crucial role in shielding cellular macromolecules from various reactive oxygen and nitrogen species fungi. Understanding GSH metabolism of vital importance for deciphering redox regulation these microorganisms. In the present study, to better understand filamentous fungi, we investigated functions dugB dugC genes model fungus Aspergillus nidulans These are orthologues dug2 dug3, which involved cytosolic degradation Saccharomyces...
Aspergillus flavus is an agriculturally and medically important filamentous fungus that produces mycotoxins, including aflatoxins, which are potent carcinogens. Here, we generated short- long-read transcript sequence data from the growth of A. strain NRRL 3357 under both typical stress conditions to produce a new annotation its genome.
The VosA-VelB hetero-dimeric complex plays a pivotal role in regulating development and secondary metabolism Aspergillus nidulans. In this work, we characterize new VosA/VelB-activated gene called vadH, which is predicted to encode 457-amino acid length protein containing four adjacent C2H2 zinc-finger domains. Mutational inactivation of vosA or velB led reduced mRNA levels vadH throughout the lifecycle, suggesting that VosA VelB have positive regulatory effect on expression vadH. deletion...
Abstract In filamentous fungi, asexual development involves morphological differentiation and metabolic changes leading to the formation of spores. The process spore in Aspergillus is precisely regulated by multiple transcription factors (TFs), including VosA, VelB, WetA, these three TFs are key regulators maturation spores (conidia) model fungus nidulans . To gain a mechanistic insight on complex regulatory roles spores, we conducted genome-wide studies expression, protein-DNA interactions,...