Aspergillus fumigatus has to cope with a combination of several stress types while colonizing the human body. A functional interplay between these different responses can increase chances survival for this opportunistic pathogen during invasion its host. In study, we shed light on how H2O2-induced oxidative response depends iron available filamentous fungus, using transcriptomic analysis, proteomic profiles, and growth assays. The applied H2O2 treatment, which induced only negligible in...

Pathogens have to cope with oxidative, iron- and carbon(glucose)-limitation stresses in the human body. To understand how combined iron–carbon limitation alters oxidative stress responses, Aspergillus fumigatus was cultured glucose–peptone or peptone containing media supplemented not deferiprone as an iron chelator. Changes transcriptome these cultures were recorded after H2O2 treatment. Responses highly dependent on availability of glucose iron. Out 16 responsive antioxidative enzyme genes,...

The b-Zip transcription factor AtfA plays a key role in regulating stress responses the filamentous fungus Aspergillus nidulans. To identify core regulons of AtfA, we examined genome-wide expression changes caused by various stresses presence/absence using A. nidulans microarrays. We also intended to address intriguing question regarding existence environmental response this important model eukaryote.

Gamma-amminobutyric acid (GABA), the major inhibitory neurotransmitter in central nervous system of vertebrates, serves as an autocrine/paracrine signaling molecule during development, modulating a number calcium (Ca(2+))-dependent processes, including proliferation, migration, and differentiation, acting via 2 types GABA receptors (GABARs): ionotropic GABA(A)Rs metabotropic GABA(B)Rs. Here, we demonstrate that mouse embryonic stem cells (mESCs), which possess capacity for virtually...

Abstract High‐frequency field potential activity between 50 and 400 Hz occurs throughout seizure‐like events recorded from the CA3 region of juvenile rat hippocampal slices under low‐[Mg 2+ ] condition. Another (400–800 Hz) component occurred mainly during preictal paroxysmal spiking onsets (97%) less frequently tonic clonic phases (38% 70%, respectively). Short epochs oscillations in this range were associated with fast negative deflections at start transients. Voltage‐clamp recordings...

To better understand the molecular functions of master stress-response regulator AtfA in Aspergillus nidulans , transcriptomic analyses atfA null mutant and appropriate control strains exposed to menadione sodium bisulfite- (MSB-), t -butylhydroperoxide- diamide-induced oxidative stresses were performed. Several elements stress response differentially expressed. Many them, including downregulation mitotic cell cycle, as MSB stress-specific upregulation FeS cluster assembly nitrate reduction,...

The contribution of stress protein duplication and deletion events to the evolution Aspergilli was studied. We performed a large-scale homology analysis proteins generated analysed three defence system models based on

Cadmium is an exceptionally toxic industrial and environmental pollutant classified as a human carcinogen. In order to provide insight into how we can keep our environment safe from cadmium contamination prevent the accumulation of it in food chain, aim elucidate Aspergillus nidulans, one most abundant fungi soil, survives handles stress. As AtfA main transcription factor governing stress responses A. examined genome-wide expression wild-type atfA null mutant exposed CdCl2. Both strains...

The bZIP transcription factors (TFs) govern regulation of development, secondary metabolism, and various stress responses in filamentous fungi. In this work, we carried out genome-wide expression studies employing Illumina RNAseq to understand the roles two AtfA AtfB Aspergillus nidulans. Comparative analyses transcriptomes control, ΔatfA, ΔatfB, ΔatfAΔatfB mutant strains were performed. Dependence a gene on (AtfB) was decided by its differential downregulation both between reference ΔatfA...

Genome-wide transcriptional changes in Aspergillus nidulans induced by nine different stress conditions were evaluated to reveal the general environmental response gene set showing unidirectional expressional under various types of stress. Clustering genes their was a useful technique for identifying large groups co-regulated genes. Altogether, 1642 co-upregulated and 3916 co-downregulated identified. Nevertheless, describe difference between transcriptomes recorded tested one chosen...

Abstract Background Fungal siderophores are likely to possess atheroprotective effects in humans, and therefore studies needed develop siderophore‐rich food additives or functional foods increase the siderophore uptake people prone cardiovascular diseases. In this study contents of mould‐ripened cheeses meat products were analysed coprogen production by Penicillium nalgiovense was characterised. Results High concentrations hexadentate fungal detected penicillia‐ripened Camembert‐...

The genome of the osmophilic Aspergillus wentii, unlike that osmotolerant nidulans, contains only gfdA, but not gfdB, glycerol 3-phosphate dehydrogenase gene. Here, we studied transcriptomic changes A. nidulans (reference strain and ΔgfdB gene deletion mutant) wentii An-gfdB expressing elicited by high osmolarity. showed a canonic hyperosmotic stress response characterized upregulation trehalose metabolism genes (including gfdB), as well high-osmolarity (HOG) map kinase pathway. gfdB caused...

To investigate the functional role of dendrites thalamocortical neurones, we have used our one-compartmental model to construct a multi-compartmental with dendritic regions chosen according representative soma-to-dendritic terminal path an X cell cat dorsal lateral geniculate nucleus. The low- threshold Ca2+ and delayed rectifier K+ channels yields more accurate results than when simulating tonic firing oscillatory activities, provides useful means for study propagation excitation on dendrites.

Glutathione (GSH) is an abundant tripeptide that plays a crucial role in shielding cellular macromolecules from various reactive oxygen and nitrogen species fungi. Understanding GSH metabolism of vital importance for deciphering redox regulation these microorganisms. In the present study, to better understand filamentous fungi, we investigated functions dugB dugC genes model fungus Aspergillus nidulans These are orthologues dug2 dug3, which involved cytosolic degradation Saccharomyces...

Th e present part of the series provides new records locally introduced native species in Hungary (Apium repens, Crypsis schoenoides, Limonium gmelinii, Rumex confertus, Sagina apetala).Th spread schoenoides and gmelinii on roadsides is due to winter salting roads, whereas appearance Apium repens apetala likely consequence use soil or peat material containing their seeds.Th reports Knautia dipsacifolia from northern Great Hungarian Plain Spiraea crenata Hungarian-Romanian-Ukrainian border...

Iron is an essential element for all microorganisms. Bacteria and fungi produce versatile siderophores binding storing this transition metal when its availability limited in the environment. The aim of study was to optimize fermentation medium Aspergillus fumigatus siderophore production. Triacetyl-fusarinine C ferricrocin yields were dependent on glucose glycine supplementations as well initial pH culture media. optimal triacetylfusarinine production contained 8% glucose, 0.4% set 5.9....