A5100665804- Traditional Chinese Medicine Analysis
- thermodynamics and calorimetric analyses
- Traditional Chinese Medicine Studies
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Ginseng Biological Effects and Applications
- Phytochemistry and biological activity of medicinal plants
- Chromatography in Natural Products
- Computational Drug Discovery Methods
- Medicinal Plant Pharmacodynamics Research
- Berberine and alkaloids research
- Essential Oils and Antimicrobial Activity
- Phytoestrogen effects and research
- Drug Transport and Resistance Mechanisms
- Healthcare and Venom Research
- Flavonoids in Medical Research
- Plant-based Medicinal Research
- Drug-Induced Hepatotoxicity and Protection
- Bioactive Compounds and Antitumor Agents
- Hepatitis C virus research
- Antibiotic Resistance in Bacteria
- Hepatitis B Virus Studies
- Alcohol Consumption and Health Effects
- Natural product bioactivities and synthesis
Chinese PLA General Hospital
2019-2025
Chinese People's Liberation Army
2012-2023
302 Military Hospital of China
2010-2020
Chengdu University of Traditional Chinese Medicine
2016
Institute of Chinese Materia Medica
2008-2014
Hubei Normal University
2009
Wuhan University
2009
PLA 306 Hospital
2008
In this study, fluorescence spectroscopy in combination with UV-vis absorption and circular dichroism (CD) was employed to investigate the high affinity binding of palmatine human serum albumin (HSA) under physiological conditions. mechanism discussion it proved that quenching HSA by is a result formation palmatine/HSA complex. Binding parameters calculating from Stern-Volmer method Scatchard showed bind affinities order 10(4) L.mol(-1). The thermodynamic studies revealed characterized...
Background: The dried root of Paeonia lactiflora Pall. (PLP) is a classical Chinese herbal medicine that has been used to treat hepatic disease for thousands years. Our previous work suggested PLP can be hepatitis with severe cholestasis. This study explored the mechanism by which affects ANIT-induced cholestasis in rats using metabolomics approach. Methods: effects on serum indices (TBIL, DBIL, AST, ALT, ALP and TBA) histopathology liver were analyzed. Moreover, UHPLC-Q-TOF was performed...
ABSTRACT Background The mechanism underlying chronic drug‐induced liver injury (DILI) remains unclear. Immune activation is a common feature of DILI progression and closely associated with metabolism. We explored the immunometabolic profile potential progression. Methods Plasma peripheral blood mononuclear cells from patients were analyzed using multiplex immunoassays untargeted metabolomics to reveal their profile. effects mechanisms DILI‐related metabolite on acute or induced by LPS CCl 4...
Cholestasis is a serious manifestation of liver diseases with limited therapies. Rhubarb, widely used herbal medicine, has been frequently at relatively large dose for treating cholestasis. However, whether doses are optimal and the therapeutic mechanism remain unclear. To explore these questions, anti-cholestatic effect five rhubarb (0.21, 0.66, 2.10, 6.60, 21.0 g/kg) in an alpha-naphthylisothiocyanate (ANIT)-induced rat model cholestasis was examined by histopathology serum biochemistry. A...
Ethnopharmacological relevance: San-Cao Granule (SCG) has been used in patients with liver fibrosis for many years and shown good effect. However, its mechanism of therapeutic action is not clear because complex chemical system. The purpose our study to establish a comprehensive systemic method that can predict the SCG antihepatic fibrosis. Materials methods: In this study, "compound–target–disease" network was constructed by combining SCG-specific fibrosis–specific target proteins...
Although berberine alkaloids (BAs) are reported to be with broad-spectrum antibacterial and antiviral activities, the interactions among BAs have not been elucidated. In present study, methicillin-resistant Staphylococcus aureus (MRSA) was chosen as a model organism, modified broth microdilution applied for determination of fluorescence absorption values calculate anti-MRSA activity BAs. We initiated four steps seek optimal combination that (1) determining single BA, (2) investigating...
Panax notoginseng (Burk.) F.H. Chen, called Sanqi in China, is a perennial herb that has been used as medicinal traditional Chinese medicine for more than 400 years. Because included many proprietary medicines, the quality of directly affects its efficacy and safety. However, considering complex special growth environment requirements notoginseng, it insufficient to evaluate based solely on analysis marker chemicals. Thus, this study, we tried with integrated indicators: (1) concentration...
Liuweiwuling Tablet (LWWL) is a licensed Chinese patent medicine (approval number: Z20060238) included in the national health insurance for anti-inflammation of chronic HBV infection, whereas its anti-HBV effect remains clarification. The study aimed to clarify antiviral and related mechanisms. HepG2.2.15 cells (wild-type HBV-replicating cells) HepG2. A64 (entecavir-resistant were used vitro test. Hydrodynamic injection-mediated mouse model was vivo Active compounds mechanisms LWWL analyzed...
Context: Various Rhizoma coptidis are commonly used in clinical applications due to their strong antibacterial effect.Objective: The effect of various R. were evaluated by microcalorimetry together with statistical analysis.Materials and methods: dried roots Coptis chinensis Franch, deltoidea C.Y. Cheng et Hsiao, teeta Wall (Ranunculaceae) identified Prof. Xiao-He Xiao. herbs tested using thermal activity monitor (TAM) air isothermal microcalorimeter, ampoule mode, evaluate the biological...
Abstract The use of threatened animals as a source traditional medicines is accelerating the extinction such species and imposes great challenges to animal conservation. In this study, we propose feasible strategy for conservation medicinal that combines trade monitoring search substitutes. First, DNA barcoding provides powerful technique species, which helps in restricting excessive illegal species. Second, pharmacological tests have been adopted evaluate biological equivalence domestic...
Abstract To identify major active constituents and measure their levels in a typical medicinal herb– Rhizoma coptidis , we applied the concept of removing adding, taking inspiration from functional genetic methods. As this herb has bacteriostatic properties is used to treat bacterial diarrhea, examined effects individual (berberine, palmatine, coptisine, epiberberine, jateorrhizine columbamine) on growth Shigella dysenteriae with microcalorimetry. The adding procedures revealed that...
This study deciphered the ameliorating effect and molecular mechanism of total glucosides White Paeony Capsules(TGP) in treatment mice model with acute lung injury(ALI) via NOD-like receptor thermal protein domain associated 3(NLRP3) signaling pathway inflammasome. The established an inflammasome activation primed bone marrow-derived macrophages(BMDMs), its was investigated by Western blot(WB), immunofluorescence staining, enzyme-linked immunosorbent assay(ELISA), flow cytometry. C57BL/6J...