In recent years the safety of probiotics has received increasing attention due to possible transfer and spread virulence factors (VFs) antibiotic resistance genes (ARGs) among microorganisms. The a strain Lactobacillus plantarum named W2 was evaluated in phenotype genotype present study. Its probiotic properties were also both vivo vitro, including adherence properties, antibacterial beneficial effects on growth immunity Pacific white shrimp, Penaeus vannamei. Hemolysis tests, tests whole...

We have examined the effect of neutrophil concentration on killing a clinical isolate Staphylococcus epidermidis . Human neutrophils at concentrations varying from 10 5 to 7 per ml were mixed in suspension with S. 3 8 colony-forming units/ml, and viable bacteria was assayed after various times 37°C. The rate bacterial depended not ratio bacteria. Below critical neutrophils, growth greater than even when 100:1. fitted time course its dependence an exponential function, exponent which is (− kp...

An increased appreciation of the importance transporter and enzyme interplay in drug clearance a desire to delineate these mechanisms necessitates utilization models that contain full complement enzymes transporters at physiologically relevant activities. Additionally, development drugs with longer half-lives requires vitro systems extended incubation times allow characterization metabolic pathways for low-clearance drugs. A recently developed coculture hepatocyte model, HepatoPac, has been...

<i>Background/Aims:</i> Although HIV-infected persons are at higher risk for acute kidney injury (AKI) during hospitalization compared with their uninfected counterparts, factors AKI not well-defined. We aimed to describe the evolving incidence of among individuals and identify important factors. <i>Methods:</i> conducted a prospective cohort study 56,823 in Department Veterans Affairs Clinical Case Registry. Outcomes were: (acute in-hospital serum creatinine increase...

Abstract Objective Racial/ethnic disparities in menopause symptoms and hormone therapy management remain understudied among women served by the Veteran’s Health Administration, despite unique racial/ethnic diversity of this population. Thus, we determined medical record-documented prescribed menopausal veterans. Methods We conducted cross-sectional analyses national Administration electronic health record data from 2014 to 2015. used logistic regression models compare medical-record...

We showed previously that the competition between bacterial killing by neutrophils and growth in stirred serum-containing suspensions could be modeled as a first-order reaction (bacterial growth) second-order neutrophils). The model provided useful parameter, critical neutrophil concentration (CNC), below which increased above it decreased, independent of initial concentration. report here this applies to bacteria three-dimensional fibrin matrices rabbit dermis. measured 103–108 colony...

To evaluate the impact of Integrated Pain Team (IPT)-an interdisciplinary chronic pain care intervention embedded in primary at a large Veterans Affairs health system. Outcomes evaluated included IPT's on perceived effectiveness care; provider self-confidence; and team satisfaction, stress, burnout.This mixed-methods quality-improvement study employed: 1) qualitative semistructured interviews 61 providers, other members, organizational stakeholders; 2) supplementary quantitative survey 65...

Abstract Background Many previous studies have reported factors that contribute to health-related quality of life (HRQoL) for a single skin disease. However, little is known about generalized associated with HRQoL across diseases. The objective this study was investigate overall HRQoL, and identify related severely impaired among patients 16 different Methods A cross-sectional 9845 disease conducted in 9 hospitals China. assessed the Chinese version Skindex-29 which measures...

Faldaprevir, an investigational agent for hepatitis C virus treatment, is well tolerated but associated with rapidly reversible, dose-dependent, clinically benign, unconjugated hyperbilirubinemia. Multidisciplinary preclinical and clinical studies were used to characterize mechanisms underlying this In vitro, faldaprevir inhibited key processes involved in bilirubin clearance: UDP glucuronosyltransferase (UGT) 1A1 (UGT1A1) (IC₅₀ 0.45 <i>µ</i>M), which conjugates bilirubin, hepatic...

Background: Human urate transporter 1 (hURAT1) is the most pivotal therapeutic target for treating hyperuricemia. However, molecular interactions between uric acid and URAT1 are still unknown due to lack of structural details. Methods: In present study, several methods (homology modeling, sequence alignment, docking, mutagenesis) were used explain atomistic mechanisms transport hURAT1. Results: Residues W357-F365 in TMD7 P484-R487 TMD11 hURAT1 have unique roles both binding causing...

The aim of this study was to establish a new paclitaxel (PTX)-resistant human esophageal squamous carcinoma (ESCC) cell line and investigate its biological characteristics. resistant (EC109/Taxol) developed in vitro by intermittent exposure the ESCC EC109 high concentration PTX with time-stepwise increment over period 6 months. MTT assay performed test drug resistance EC109/Taxol cells. morphological features were observed using inverted microscopy apoptosis measured flow cytometry (FCM)...

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) technology is widely used as a tool for gene editing in rat genome site-specific engineering. Multidrug resistance 1 [MDR1 (also known P-glycoprotein)] key efflux transporter that plays an important role not only the transport of endogenous and exogenous substances, but also tumor MDR. In this report, novel MDR1 (<i>Mdr1a/b</i>) double-knockout (KO) model was generated by...

The Hsp70 gene plays an important role in the animal immune response. In order to analyze linkage between genetic polymorphisms of and virus-resistance trait shrimp, we detected SNPs coding region by direct sequencing 104 specimens Litopenaeus vannamei from three shrimp populations (one TSV-resistance line two TSV-susceptibility lines). Five were found including 661C/A, 712T/C, 782C/T, 892C/T, 1090C/T fragment (GenBank accession no. AY645906). There significant difference allelic frequency...

Inhibitor of growth 1 (ING1) is a type II tumor suppressor that affects cell function by altering chromatin structure and regulating transcription. Recently, three ING1 splice variants have been cloned, but their roles in apoptosis p53 regulation human hepatocellular carcinoma (HCC) not fully elucidated. The present study found ING1, variant-dependent manner, inhibited hepatoma proliferation colony formation, induced cycle arrest at G0/G1 phase, postponed formation nude mice. Expression...

Faldaprevir is a hepatitis C virus protease inhibitor that effectively reduces viral load in patients. Since faldaprevir exhibits slow metabolism vitro and low clearance vivo, was expected to be minor pathway. The human [¹⁴C] absorption, distribution, metabolism, excretion study revealed two monohydroxylated metabolites (M2a M2b) were the most abundant excretory feces, constituting 41% of total administered dose. To deconvolute formation disposition M2a M2b humans determine why...

Glucose transporter 9 (GLUT9), which transports urate in an electrogenic and voltage-dependent manner, plays important role the maintenance of normal blood uric acid/urate levels. In present study, we established a cell model based on single-electrode patch-clamp technique for characterization GLUT9 explored inhibitory effects benzobromarone (BM) probenecid (PB) urate-induced currents mouse GLUT9a (mGLUT9a)-expressing HEK-293T cells. The results showed that acid, rather than glucose...

Opioid use and chronic pain are prevalent in the veteran population. Collaborative care enhances coordination between patients their teams, motivational interviewing (MI) is a communication style designed to facilitate behavior change. This study evaluated of collaborative with MI (CCMI) high-risk prescription opioid use.Small pilot randomized controlled trial.An urban Veterans Affairs (VA) Medical Center United States.One hundred adult veterans currently enrolled into primary receiving...

Ovarian cancer (OC) is still the leading aggressive and lethal disease of gynecological cancers, platinum-based regimes are standard treatments. However, nearly 20%-30% patients with OC initial platinum resistant (IPR), there a lack valid tools to predict whether they will be primary or not prior chemotherapy.Transcriptome data from The Cancer Genome Atlas (TCGA) was downloaded as training data, transcriptome GSE15622, GSE102073, GSE19829, GSE26712 were retrieved Gene Expression Omnibus...

ABSTRACT The pharmacokinetics, mass balance, and metabolite profiles of faldaprevir, a selective peptide-mimetic hepatitis C virus NS3/NS4 protease inhibitor, were assessed at steady state in 7 healthy male subjects. Subjects received oral doses 480 mg faldaprevir on day 1, followed by 240 days 2 to 8 10 15. [ 14 C]faldaprevir (240 containing 100 μCi) was administered 9. Blood, urine, feces, saliva samples collected intervals throughout the study. Metabolite profiling performed using...