A5085470464- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Galician and Iberian cultural studies
- Urbanism, Landscape, and Tourism Studies
- Cardiac tumors and thrombi
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Transport and Economic Policies
- Archaeological and Historical Studies
- Occupational Health and Safety in Workplaces
- Environmental and Ecological Studies
- Chronic Lymphocytic Leukemia Research
- CRISPR and Genetic Engineering
- Historical and socio-economic studies of Spain and related regions
- Chronic Myeloid Leukemia Treatments
- Fire effects on ecosystems
- Heat shock proteins research
- Ecology and Vegetation Dynamics Studies
- Virus-based gene therapy research
- Regional Development and Innovation
- Maritime Ports and Logistics
- Cancer-related gene regulation
- Immunodeficiency and Autoimmune Disorders
- Engineering and Information Technology
Universidad de Salamanca
2010-2023
Instituto de Investigación Biomédica de Salamanca
2012-2023
Centro de Investigación del Cáncer
2012-2021
University of Chile
2021
Instituto de Biomedicina de Sevilla
2015-2018
Hospital Universitario Virgen del Rocío
2015-2018
Consejo Superior de Investigaciones Científicas
2008-2016
Universidad de Sevilla
2015
Universidad Nacional Autónoma de México
1995-2014
Mackay Memorial Hospital
2012
AbstractFire has favored pines throughout their natural range in environments subject to continuous disturbances, such as the Mediterranean Basin. However, recovery of pine species after large fires is not always successful. In this study, we analyze post-fire regeneration pattern Pinus halepensis, P. nigra and sylvestris three years fire, an area affected by a wildfire 1994. Moreover, develop model succession predict medium-term changes forest composition 30 fire from monitored during first...
Abstract Purpose: Ewing tumor cell survival and proliferation depends on several autocrine loops. Targeting these loops is a promising therapeutic approach. We recently showed the cytostatic role of imatinib, an inhibitor SCF-KIT loop, cells, in this study, we intend to analyze inhibition insulin-like growth factor I receptor (IGF1R) loop. Experimental Design: analyzed IGF1R blockade by ADW742, small molecule specific for receptor, alone combination with vincristine, doxorubicin lines....
BACKGROUND: Ewing sarcoma is a paradigm of solid tumour -bearing chromosomal translocations resulting in fusion proteins that act as deregulated transcription factors. fuse the EWS gene with an ETS factor, mainly FLI1. Most EWS–FLI1 target genes still remain unknown and many have been identified heterologous model systems. METHODS: We developed stable RNA interference knocking down cell line TC71. Gene expression analyses were performed to study effect on genetic signature identify could...
// José Luis Ordóñez 1,4,* , Ana Teresa Amaral Angel M. Carcaboso 2 David Herrero-Martín 3 María del Carmen García-Macías 4 Vicky Sevillano Diego Alonso Guillem Pascual-Pasto Laura San-Segundo Monica Vila-Ubach Telmo Rodrigues Susana Fraile Cristina Teodosio Agustín Mayo-Iscar 5 Miguel Aracil 6 Carlos Galmarini Oscar Tirado Jaume Mora and Enrique de Álava 1,4 1 Laboratory of Molecular Pathology, Instituto Biomedicina Sevilla (IBiS), Hospital Universitario Virgen Rocio/CSIC/Universidad...
Abstract Ewing Sarcoma (ES) shows several deregulated autocrine loops mediating cell survival and proliferation. Therefore, their blockade is a promising therapeutic approach. We previously reported the in vitro effect of insulin-like growth factor 1 receptor (IGF1R)/KIT pathway on ES lines, we now extend our observations to changes induced by this treatment interacting proteins/networks. A proteomic analysis revealed that Heat Shock Protein (HSP)90 was differentially expressed between lines...
Background Ewing Sarcoma (EWS) is a mesenchymal-derived tumor that generally arises in bone and soft tissue. Intensive research regarding the pathogenesis of EWS has been insufficient to pinpoint early events sarcomagenesis. However, Mesenchymal Stem Cell (MSC) currently accepted as most probable cell origin. Materials Methods In an initial study deep characterization MSC obtained specifically from patients (MSC-P), we compared them with derived healthy donors (MSC-HD) lines. We evaluated...
This study analyses the effects of post‐dispersal predation Pinus nigra seeds on initial recruitment this species in areas burned by large wildfires, where P. shows very low regeneration. In three different habitats obtained a gradient time since fire Catalonia (NE Spain), we have evaluated seed predators (ants, rodents and birds) removal early seedling establishment using selective exclosures limiting their access to seeds. Ants were most efficient predator group, followed birds. The...
Receptor endocytosis is critical for cell signaling. IGF1R mediates an autocrine loop that de-regulated in Ewing Sarcoma (ES) cells. Here we study the impact of internalization, mediated by clathrin and caveolin-1 (CAV1), ES We used CAV1-siRNA to interfere clathrin- caveolin-dependent endocytosis. Chlorpromazine (CPMZ) methyl-beta-cyclo-dextrin (MCD) were also order inhibit endocytosis, respectively. analyzed internalization co-localization with CAV1 upon ligand binding, as well status...
Abstract Purpose: Goal of this study was to identify mechanisms that limit efficacy trabectedin (ET-743, Yondelis) in Ewing sarcoma (EWS), so as develop a clinical applicable combination therapy. Experimental Design: By chromatin immunoprecipitation, we analyzed EWS–FLI1 binding the promoters several target genes, such TGFβR2, CD99, insulin-like growth factor receptor 1 (IGF1R), and IGF1, both vitro xenografts treated with or doxorubicin. Combined therapy anti-IGF1R agents (AVE1642 HAb;...
Neuroblastic tumors (NTs) include the neuroblastomas, ganglioneuroblastomas and ganglioneuromas. We have reported previously that calcium-sensing receptor is expressed in differentiated, favorable NTs but almost undetectable unfavorable neuroblastomas. now detected hypermethylation of a particular region within CpG island encompassing CaSR gene promoter 2 neuroblastoma cell lines 25% primary Hypermethylation this was associated with reduced messenger RNA expression several predictors poor...
CRISPR/Cas9 technology was used to abrogate p210 oncoprotein expression in the Boff-p210 cell line, a pro-B line derived from interlukin-3-dependent Baf/3, that shows IL-3-independence arising constitutive of BCR-ABL p210. Using this approach, pools Boff-p210-edited cells and single edited cell-derived clones were obtained functionally studied vitro. The loss resulted ability grow absence IL-3, as Baf/3 parental showing significantly increased apoptosis levels. Notably, clone carrying...
Endoglin (ENG; CD105) is a coreceptor of the TGFβ family that highly expressed in proliferating endothelial cells. Often coopted by cancer cells, ENG can lead to neo-angiogenesis and vasculogenic mimicry aggressive malignancies. It exists both as transmembrane cell surface protein, where it primarily interacts with TGFβ, soluble matricellular protein (sENG) when cleaved matrix metalloproteinase 14 (MMP14). High expression has been associated poor prognosis Ewing sarcoma, an bone occurs...
Abstract The deletion of 11q (del(11q)) invariably comprises ATM gene in chronic lymphocytic leukemia (CLL). Concomitant mutations this the remaining allele have been identified 1/3 CLL cases harboring del(11q), being biallelic loss associated with adverse prognosis. Although introduction targeted BCR inhibition has significantly favored outcomes del(11q) patients, responses patients functional through inactivation are unexplored, and development resistances to therapies increasingly...
In order to evaluate the effect of training upon postoperative adhesions, standard bipolar and mechanical, nonopposing injuries were performed in uterine horns side walls 52 mature female rabbits using a conventional three-puncture laparoscopy, by an endoscopic surgeon with limited experience. An additional injury, either or mechanical both, was retro-uterine space. With experience, duration surgery decreased progressively from 12 +/- 2 8 1 min first last 10 animals respectively. The amount...
:Pinus nigra subsp. salzmannii is a pine species that regenerates quite well under its own canopy but has difficulty regenerating after fire. This study analyzes, by means of greenhouse and field experiments, the pattern seed germination seedling establishment P. in gradient post-fire environmental conditions. The results obtained controlled conditions experiment cover effects absence vegetation interference indicate survival growth attained their maximum values high levels. In field, were...
Background: The t(12;21)(p13;q22), which fuses ETV6 and RUNX1 genes, is the most common genetic abnormality in children with B-cell precursor acute lymphoblastic leukaemia. implication of fusion protein leukemogenesis seems to be clear. However, its role maintenance disease continues controversial. Methods: Generation an vitro ETV6/RUNX1 knock out model using CRISPR/Cas9 gene editing system. Functional characterization by RNA sequencing, proliferation assays, apoptosis pharmacologic studies,...