A5032162886- Hearing, Cochlea, Tinnitus, Genetics
- Cancer-related molecular mechanisms research
- Developmental Biology and Gene Regulation
- Corneal surgery and disorders
- Genomics and Chromatin Dynamics
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Aquaculture Nutrition and Growth
- Neuroscience of respiration and sleep
- Olfactory and Sensory Function Studies
- Medical Imaging and Pathology Studies
- Intraocular Surgery and Lenses
- Fish Biology and Ecology Studies
- Asthma and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Corneal Surgery and Treatments
- Genetics and Neurodevelopmental Disorders
- Eicosanoids and Hypertension Pharmacology
- Occupational and environmental lung diseases
- Ocular Infections and Treatments
- Nanoplatforms for cancer theranostics
- RNA regulation and disease
- Yersinia bacterium, plague, ectoparasites research
- Photodynamic Therapy Research Studies
- Forensic Toxicology and Drug Analysis
University of Southern California
2019-2024
University of California, San Diego
2020-2024
Hainan University
2024
Chongqing Cancer Hospital
2023
Chongqing University
2023
Broad Center
2020-2021
Dalian University
2021
Dalian University of Technology
2021
Agency for Science, Technology and Research
2016
Institute of Bioengineering and Nanotechnology
2016
The human lung plays vital roles in respiration, host defense, and basic physiology. Recent technological advancements such as single-cell RNA sequencing genetic lineage tracing have revealed novel cell types enriched functional properties of existing lung. time has come to take a new census. Initiated by members the NHLBI-funded LungMAP Consortium aided experts biology community, we synthesized current data into comprehensive practical cellular census Identities normal are captured...
Exaggerated airway constriction triggered by repeated exposure to allergen, also called hyperreactivity, is a hallmark of asthma. Whereas vagal sensory neurons are known function in allergen-induced hyperreactivity
The mechanoreceptive sensory hair cells in the inner ear are selectively vulnerable to numerous genetic and environmental insults. In mammals, lack regenerative capacity, their death leads permanent hearing loss vestibular dysfunction. Their paucity inaccessibility has limited search for otoprotective strategies. Growing vitro would provide a route overcome this experimental bottleneck. We report combination of four transcription factors (Six1, Atoh1, Pou4f3, Gfi1) that can convert mouse...
During embryonic development, hierarchical cascades of transcription factors interact with lineage-specific chromatin structures to control the sequential steps in differentiation specialized cell types. While examples factor have been well documented, mechanisms underlying developmental changes accessibility type-specific enhancers remain poorly understood. Here, we show that transcriptional "master regulator" ATOH1-which is necessary for two distinct mechanoreceptor types, hair cells inner...
The mammalian cochlea loses its ability to regenerate new hair cells prior the onset of hearing. In contrast, adult vestibular system can produce in response damage, or by reprogramming supporting with cell transcription factor Atoh1. We used RNA-seq and ATAC-seq probe transcriptional epigenetic responses utricle damage Atoh1 transduction. show that regenerative correlates a more accessible chromatin structure compared their cochlear counterparts. also provide evidence transduction is able...
The specification of cartilage requires Sox9, a transcription factor with broad roles for organogenesis outside the skeletal system. How Sox9 and other factors gain access to cartilage-specific cis-regulatory regions during development was unknown. By analyzing chromatin accessibility differentiation neural crest cells into chondrocytes zebrafish head, we find that cartilage-associated is dynamically established. Cartilage-associated become accessible after migration are co-enriched Fox...
Lidocaine has been reported to induce apoptosis on rabbit corneal endothelial cells. However, the apoptotic effect and exact mechanism involved in cytotoxicity of lidocaine are not well-established human (HCE) In this study, we investigated apoptosis-inducing HCE cells vitro. After were treated with at concentrations 0.15625-10.0 g/l, morphology ultrastructure observed by inverted light microscope transmission electron (TEM). Cell viability was measured MTT assay, ratio evaluated flow...
Significance The evolution of jaws in early vertebrates provided such a predatory advantage that 99% vertebrate species living today are jawed. What is often overlooked, however, another structural innovation happened concurrently and may have been equally critical to the lineage’s success: musculoskeletal gill covers actively drive oxygenated water over gills. Here, we identify first essential gene for cover formation modern vertebrates, Pou3f3, uncover genomic element brought Pou3f3...
To evaluate the biological functions of tissue-engineered human corneal epithelium (TE-HCEP) by transplantation in limbal stem cell deficiency (LSCD) rabbit models.TE-HCEPs were reconstructed with DiI-labeled untransfected HCEP cells and denuded amniotic membrane (dAM) air-liquid interface culture, their morphology structure characterized hematoxylin-eosin (HE) staining paraffin-sections, immunohistochemistry electron microscopy. LSCD models established mechanical alcohol treatment left eyes...
To demonstrate the apoptosis-inducing effect of lidocaine on human corneal stromal (HCS) cells in vitro, and provide experimental basis for safety anesthetic usage clinic ophthalmology.In vitro cultured HCS were treated with at different doses times, their morphology was monitored successively inverted phase contrast microscopy. The membrane permeability them detected by acridine orange/ethidium bromide (AO/EB) double staining. DNA fragmentation examined agarose gel electrophoresis,...
Abstract Chronic exposure of the lung to irritants such as allergen is a primary cause asthma characterized by exaggerated airway constriction, also called hyperreactivity, which can be life-threatening. Aside from immune cells, vagal sensory neurons are important for hyperreactivity 1–4 . However, identity and signature downstream nodes this adaptive circuit remains poorly understood. Here we show that single population Dbh + in nucleus solitary tract (nTS) brainstem, ambiguous (NA), both...
Introduction Asian tropical rainforests have the highest rates of degradation in world. Consequently, a large decline Chinese Vatica mangachapoi (a keystone species) had led to its listing category “vulnerable” species by IUCN. However, current status after decades conservation efforts remains unknown. Methods Here, we evaluate V. . Results and Discussion We found that population is now dispersed 14 protected areas, largest being coastal forest contains 96.84% all Compared their historic...
SUMMARY Idiopathic pulmonary fibrosis (IPF) is a lethal progressive lung disease urgently needing new therapies. Current treatments only delay progression, leaving transplant as the sole remaining option. Recent studies support model whereby IPF arises because alveolar epithelial type II (AT2) cells, which normally mediate distal regeneration, acquire airway and/or mesenchymal characteristics, preventing proper repair. Mechanisms driving this abnormal differentiation remain unclear. We...
Summary Whereas the gill chambers of extant jawless vertebrates (lampreys and hagfish) open directly into environment, jawed evolved skeletal appendages that promote unidirectional flow oxygenated water over gills. A major anatomical difference between two vertebrate lineages is presence a single large cover in bony fishes versus separate covers for each chamber cartilaginous fishes. Here we find these divergent patterns correlate with pharyngeal arch expression Pou3f3 orthologs. We identify...
Abstract The specification of cartilage requires Sox9, a transcription factor with broad roles for organogenesis outside the skeletal system. How Sox9 gains selective access to cartilage-specific cis-regulatory regions during development had remained unclear. By analyzing chromatin accessibility differentiation neural crest cells into chondrocytes zebrafish head, we find that cartilage-associated is dynamically established. Cartilage-associated become accessible after migration are...