A5101886280- Protein Degradation and Inhibitors
- Cancer-related gene regulation
- Prostate Cancer Treatment and Research
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- PARP inhibition in cancer therapy
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cell death mechanisms and regulation
- Circular RNAs in diseases
- Cancer, Lipids, and Metabolism
- GDF15 and Related Biomarkers
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Heavy Metal Exposure and Toxicity
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Peptidase Inhibition and Analysis
- Chromium effects and bioremediation
- Intraocular Surgery and Lenses
- Leaf Properties and Growth Measurement
- Phytochemicals and Antioxidant Activities
Nanjing Medical University
2022-2024
North China University of Science and Technology
2024
Wuxi People's Hospital
2023-2024
University of Kentucky
2019-2024
Fudan University
2024
Sichuan University
2024
State Key Laboratory of Oral Diseases
2024
China University of Geosciences (Beijing)
2024
Ningxia Medical University
2024
Huazhong University of Science and Technology
2021-2023
Enzalutamide, approved by the United States Food and Drug Administration in 2018 for management of metastatic castration-resistant prostate cancer (CRPC), is an androgen receptor (AR) inhibitor. It blocks binding to AR, AR nuclear translocation, AR-mediated DNA binding. Unfortunately, a considerable proportion tumors eventually develop resistance during treatment. The molecular mechanisms underlying enzalutamide are not completely understood. Enhancer zeste homolog 2 (EZH2), catalytic...
Abstract Background Malnutrition is common in patients with acute myocardial infarction (AMI) and associated a poor prognosis. The prognostic value of the nutritional index (PNI) AMI remains controversial. We aimed to explore relationship between PNI all-cause mortality critically ill evaluate incremental commonly used assessment tools. Methods Medical Information Mart for Intensive Care-IV (MIMIC-IV) database was conduct retrospective cohort analysis on 1180 AMI. primary endpoints were...
Abstract Olaparib is an FDA-approved PARP inhibitor (PARPi) that has shown promise as a synthetic lethal treatment approach for BRCA-mutant castration-resistant prostate cancer (CRPC) in clinical use. However, emerging data have also even cells may be resistant to PARPi. The mechanistic basis these drug resistances poorly understood. Polo-like kinase 1 (Plk1), critical regulator of many cell-cycle events, significantly elevated upon castration mice carrying xenograft tumors. Herein, by...
We report the scalable production of recombinant proteins in Escherichia coli, reliant on tightly controlled autoinduction, triggered by phosphate depletion stationary phase. The method, engineered strains and plasmids, enables improved protein expression across scales. Expression levels using this approach have reached as high 55% total cellular protein. initial use method instrumented fed-batch fermentations cell densities ∼30 gCDW/L titers up to 8.1 ± 0.7 g/L (∼270 mg/gCDW). process has...
Essential trace elements (ETEs) play essential roles in vital functions, but their effects on epigenetic aging remain poorly understood.This study aimed to investigate the associations of ETEs with four indicators and assess potential mediating role inflammation.We recruited 93 individuals from hospitals between October 2018 August 2019. Plasma levels cobalt, copper, iron, manganese, molybdenum, selenium, zinc were measured by ICP-MS, leukocyte DNA methylation using Illumina MethylationEPIC...
Polo-like kinase 1 (Plk1), a crucial regulator of cell-cycle progression, is overexpressed in multiple types cancers and has been proven to be potent promising target for cancer treatment. In case prostate cancer, we once showed that antineoplastic activity Plk1 inhibitor largely due inhibition androgen receptor (AR) signaling. However, also discovered causes activation the β-catenin pathway increased expression c-MYC, eventually resulting resistance inhibition. JQ1, selective small-molecule...
Abstract Background Because androgen receptor (AR) signaling is essential for prostate cancer (PCa) initiation and progression, castration the main approach treatment. Unfortunately, patients tend to enter a stage called castration‐resistant (CRPC) despite initial response castration. For various reasons, AR reactivated in CRPC. As such, inhibitors, such as enzalutamide, has been approved by Food Drug Administration treat CRPC clinic. However, limited success of these new drugs suggests an...
Gemcitabine is the standard-of-care for chemotherapy in patients with pancreatic adenocarcinoma and it can directly incorporate into DNA or inhibit ribonucleotide reductase to prevent replication and, thus, tumor cell growth. Most tumors, however, develop resistance gemcitabine. Polo-like kinase 1 (Plk1), a critical regulator many cycle events, significantly elevated human cancer. In this study, we show that Plk1 required G1/S transition inhibition of reduces synthesis rate cancer cells....
We report improved release of recombinant proteins in Escherichia coli, which relies on combined cellular autolysis and DNA/RNA autohydrolysis, conferred by the tightly controlled autoinduction both phage lysozyme nonspecific endonuclease from Serratia marcescens. Autoinduction occurs a two-stage process wherein heterologous protein expression enzymes are induced upon entry into stationary phase phosphate depletion. Cytoplasmic periplasmic kept inducing lysis until membrane integrity is...
Lidocaine has been reported to induce apoptosis on rabbit corneal endothelial cells. However, the apoptotic effect and exact mechanism involved in cytotoxicity of lidocaine are not well-established human (HCE) In this study, we investigated apoptosis-inducing HCE cells vitro. After were treated with at concentrations 0.15625-10.0 g/l, morphology ultrastructure observed by inverted light microscope transmission electron (TEM). Cell viability was measured MTT assay, ratio evaluated flow...
Upregulation of EZH2 is associated with advanced stage and poor prognosis prostate cancer; therefore, it likely to be a promising therapeutic target. Metformin, drug that has been used treat type 2 diabetes, was found have antineoplastic activity in different cancers. Herein, we report the combination metformin inhibitor GSK126 exerts synergistic inhibition on cancer cell growth, both vitro vivo Mechanistically, identify can reduce expression through upregulating miR-26a-5p, which...
Circular RNAs (circRNAs) play a critical role in the modulation of tumor metabolism. However, expression patterns and metabolic function circRNAs chronic lymphocytic leukemia (CLL) remain largely unknown. This study aimed to elucidate lipid metabolism CLL.The cohort 53 patients with CLL were investigated using whole transcriptome sequencing. Cell viability, liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis, Nile red staining as well triglyceride (TG) assay used evaluate...
Tumor cells resist oxidative damage and apoptosis by activating defense mechanisms. Herein, a self-delivery biomedicine (designated as BSC) is developed the self-assembly of Bortezomib (BTZ), Sabutoclax (Sab) Chlorin e6 (Ce6). Interestingly, BTZ can be coordinated with Sab to promote assembly uniform ternary through non-covalent intermolecular interactions. Moreover, proteasome inhibitor prevent tumor from scavenging damaged proteins reduce their resistance. downregulate B-cell lymphoma 2...
Constipation is a common functional gastrointestinal disorder, which needs more effective treatment approaches. Houpo Paiqi Mixture (HPPQM) type of Chinese patent medicine developed from classical formula that has been widely applied to the intestinal motility disorder. Here we aim assess effectiveness HPPQM in constipation rat models and its potential mechanism.UPLC-MS/MS was performed investigate chemical component HPPQM. Rats were randomly divided into normal control, model (CM), (low,...
Cavernous hemangioma and schwannoma are tumors that both occur in the orbit. Because treatment strategies of these two different, it is necessary to distinguish them at initiation. Magnetic resonance imaging (MRI) typically used differentiate tumor types; however, they present similar features MRI images which increases difficulty differential diagnosis. This study aims devise develop an artificial intelligence framework improve accuracy clinicians' diagnoses enable more effective decisions...
In this work, we developed a carrier free photodynamic oxidizer to decrease intracellular glutathione (GSH) levels and disrupt the redox homeostasis for enhanced therapy (PDT) efficacy on tumor inhibition.
Abstract The copolymers P(AM‐ co ‐4VP) of acrylamide (AM) and 4‐ vinyl pyridine (4‐VP) were synthesized. They had serial varied composition molecular weight then quaternarized by using dimethyl sulfate. Two series cationic polyacrylamide quaternary salt‐type(Quaternary ‐4VP)'QPAV) been synthesized, which have different degree respectively. In this paper, the antibacterial property QPAV was mainly investigated Escherichia coli ( E. ) as model bacterium. Influences various factors, such...
Prostate cancer (PCa) is the most commonly diagnosed malignancy among men, and progression of this disease results in fewer treatment options available to clinical patients. It highlights vital necessity for discovering novel therapeutic approaches expanding current understanding molecular mechanisms. Epigenetic alternations such as DNA methylation models histone modifications have been associated key drivers development advancement PCa. Several studies conducted demonstrated that targeting...