Objectives: Diabetic nephropathy (DN) is a major complication of diabetes that requireseffective treatment options. This study explores the potential benefits saffron extractas remedy derived from medicinal plants, focusing on its effects key inflammatorygenes—Toll-like receptor 4 (TLR-4), S100 calcium-binding protein A8 (S100A8), andHigh Mobility Group Box 1 (HMGB1)—as well as role in reducing oxidative stressin kidney tissue rats with type diabetes. Methods: The were randomly divided into...

Background: The high incidence of Non-Alcoholic Fatty Liver Disease (NAFLD) as a hepatic component metabolic syndrome is attributed to the ongoing rise in obesity rates. Given beneficial effects hydroxychloroquine (HCQ) on metabolism, there growing interest combining it with fisetin (FSN), natural product, treat NAFLD more effectively. Objective: efficacy and safety combined therapy FSN HCQ animal models were assessed, focusing liver function, insulin sensitivity, oxidative stress,...

In this study, we aimed to investigate the effects of 10 weeks high-intensity interval training (HIIT) and endurance (END) on irisin, betatrophin, insulin, fasting blood glucose (FBG) concentrations, lipid profiles in diabetic rats.Twenty-four Wistar rats (weight: 200-250 g) were randomly assigned into four groups as follows: (1) control (Cnt), (2) (Dibt), (3) HIIT (Dibt-HIIT), (4) END (Dibt-END). For inducing diabetes, after 12 h food starvation, nicotinamide (120 mg/kg) streptozotocin...

Despite the dramatic efficacy of erlotinib, an EGFR tyrosine kinase inhibitor (TKI), most non-small cell lung cancer (NSCLC) patients ultimately acquire resistance to this agent. Different studies indicated that miRNA-125a-5p is down-regulated in human cells and may function as a tumor suppressor by targeting EGFR. However, biological NSCLC EGFR-TKIs not fully understood. In study effect on proliferation, apoptosis sensitivity A549 erlotinib was investigated.After transfection, expression...

Studies have shown that myeloid cell leukemia-1 (Mcl-1) is the target gene for microRNA -101 (miRNA-101), and decreased levels of miRNA-101 are associated with elevated Mcl-1 lung cancer survival. The objective present study was to investigate effect on sensitivity A549 cells etoposide.The conducted during 2018 2019 at Arak University Medical Sciences, Arak, Iran. expression assessed using reverse transcription-quantitative polymerase chain reaction 3-(4, 5-Dimethylthiazol-2-yl)-2,...

One of the major complications diabetes is diabetic nephropathy, and often many patients suffer from nephropathy. That why it important to find mechanisms that cause nephropathy its treatment. This study was designed examine antidiabetic effects biochanin A (BCA) evaluate on oxidative stress markers expression transforming growth factor-β1 (TGF-β1) protease-activated receptors-2 (PAR-2) genes in kidney type 1 rats. After induction using streptozotocin (STZ), 55 mg/kg bw dose, rats were...

Background: Despite the dramatic efficacy of ABT-737, a large percentage cancer cells ultimately become resistance to this drug. Evidences show that over-expression Mcl-1 is linked ABT-737 in NSCLC cells. The aim study was investigate effect miRNA-101 on expression and sensitivity A549 ABT-737. Methods: After transfection, mRNA levels were quantified by RT-qPCR. Trypan blue staining used explore cell growth. cytotoxic effects alone combination, measured using MTT assay. drugs combination...

Abstract Objectives To find suitable biomarkers for diagnosis of prostate cancer (PC) in serum and saliva; also, to evaluate the diagnostic efficacy saliva patients with PC. Methods This case-control study included 20 PC benign prostatic hyperplasia (BPH). Blood were collected from participants centrifuged. Serum supernatant used biochemical analysis. We evaluated salivary levels urea, creatinine, prostate-specific antigen (PSA), creatine kinase BB (CK-BB), zinc, β-2 microglobulin (B2M),...

TNF-α and S100B are important signaling factors that involved in many aberrant conditions of the brain. Chronic morphine exposure causes modifications brain.We examined consequences chronic consumption by parents before mating on hippocampus levels their offspring.A total 12 adult female male Wistar rats were used as parents. Each gender was divided randomly into two groups: control consumer. Morphine consumer groups received sulfate dissolved drinking water (0.4 mg/ml) for 60 days. Control...

Background: Despite effective activity of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), such as erlotinib, all non-small cell lung cancer (NSCLC) patients eventually acquire resistance to these agents. Studies have demonstrated that down-regulation miRNA-145 leads enhancement EGFR expression, proliferation and metastasis. The aim this study was investigate the effect on sensitivity A549 NSCLC cells erlotinib. Methods: Quantitative real-time PCR used examine...

Abstract Objective To find suitable biomarkers for diagnosis of Breast cancer in serum and saliva; also, to examine the correlation between salivary concentrations biomarkers. Methods This case-control study included 30 women with breast as a case group healthy matched control group. Blood saliva specimens were collected from all participants. We evaluated antigen 15-3 (CA15-3), carcinoembryonic (CEA), estradiol, vaspin, obestatin concentrations. Mann-Whitney U testing Spearman coefficients...

Abstract Background The present study was conducted to examine the antidiabetic effects of Scrophularia striata ethanolic extract and evaluate its on oxidative stress markers RAGE S100A8 gene expressions in kidney type 1 diabetic rats. Methods A total 36 rats (weight 200–250 g) were randomly assigned into six groups as follows: Cnt, Cnt + S. 100, 200 that received normal saline, 100 mg/kg bw, bw ethanol , respectively; group Dibt, Dibt respectively. Type diabetes induced by a single...

Introduction: Up-regulation of the anti-apoptotic proteins such as Mcl-1 is associated with primary and secondary resistance tumor cells to ABT-737 Bcl-2 inhibitor. The combined treatment inhibitors has been proposed an attractive therapeutic strategy overcome this drug resistance. Here, we investigated effect dihydroartemisinin on expression sensitization T-ALL ABT-737. Methods: cell growth survival were tested by proliferation MTT assays, respectively. mRNA levels Bcl-2, Mcl-1, Bax P21...

Introduction: Change in the balance of Bcl-2 family proteins is one main reasons for resistance tumor cells to ABT-199. In this study, effect dihydroartemisinin on cell growth, apoptosis and sensitivity AML ABT-199 was investigated. Methods: Cell proliferation survival were assessed by trypan blue staining MTT assay, respectively. measured Hoechst 33342 caspase-3 activity assay. The expression levels Bcl-2, Mcl-1 Bax mRNA tested qRT-PCR. Results: Our data showed that combination therapy...

Enhanced cell survival and drug resistance in tumor cells have been linked to the overexpression of antiapoptotic members Bcl-2 family proteins, including Mcl-1. The aim this study was explore impact formononetin dihydroartemisinin combination on growth apoptosis acute myeloid leukemia (AML) cells.

Abstract Despite the dramatic efficacy of EGFR‐TKIs, most non‐small cell lung cancer patients ultimately develop resistance to these agents. In this study, we explored effects miRNA‐125a‐5p and miRNA‐145, alone or in combination, EGFR expression, growth sensitivity NSCLC cells erlotinib. The expression was measured using RT‐qPCR Western blotting. effect miRNAs erlotinib on survival assessed by trypan blue assay MTT assay, respectively. Apoptosis ELISA death assay. We found that transfection...

Diabetic nephropathy is one of the major complications diabetes, use medicinal plants increasing due to fewer side effects. This study was designed examine antidiabetic effects Allium jesdianum (A. jesdianum) ethanolic extract and evaluate its on oxidative stress markers expression connective tissue growth factor (CTGF) receptor for advanced glycation endproducts (RAGE) genes in kidney type 1 diabetic rats.In this study, we randomly divided 24 rats into four groups with six each group as...

Abstract Objectives Liver cirrhosis is one of the most important causes death from liver diseases. Nowadays, use herbal medicines has increased due to its availability, less side effects and cheapness for treatment The present study was conducted examine therapeutic hydroalcoholic extract Scrophularia striata ( S. ) on thioacetamide-induced in rats through evaluate oxidative stress markers expression metalloproteinase 1 (TIMP 1), toll-like receptor-4 (TLR-4), Mitofusin (MFN2) genes. Methods...

Abstract Background Physical inactivity is the major risk factor for type 2 diabetes (T2D). The present study was conducted to investigate effects of resistance training and endurance on diabetic-related metabolic parameters in diabetic rats. Materials methods Twenty-four male Wistar rats were randomly assigned four groups six each: control group (C), (D), (RES) (END). T2D induced intraperitoneally using nicotinamide (120 mg/kg) streptozotocin (STZ, 65 mg/kg). period 70 days. irisin,...