BPA has been reported to leach from some resin based dental restorative materials and used for orthodontic treatment. To confirm update previous findings, especially in light of the new temporary lower threshold value tolerable daily intake, we have investigated leaching 4 composite filling materials, 3 sealants 2 bonding materials. The were either uncured dissolved methanol or cured. cured kept deionized water 24 hours weeks. Samples subsequently analyzed by ultra-performance liquid...

Polymer‐based dental restorative materials are designed to polymerize in situ. However, the conversion of methacrylate monomer polymer is never complete, and leakage occurs. It has been shown that these monomers toxic vitro; hence concerns regarding exposure patients personnel have raised. Different methacrylates thought cause toxicity through similar mechanisms, sequestration cellular glutathione (GSH) may be a key event. In this study we examined commonly used methacrylates,...

We have investigated the potential of two complex mineral particles (feldspar and mylonite), quartz (Min-U-Sil), suspended particulate matter (SRM-1648) (SPM) from urban air to induce inflammatory cytokine responses in primary rat alveolar type 2 cells macrophages, involvement cellular formation free radicals these responses. All particle types induced an increased release interleukin (IL)-6 macrophage protein (MIP)-2 cells. Diphenyleneiodonium chloride (DPI), a selective inhibitor...

Methacrylate monomers that are found to leach from cured resin-based dental materials induce biological effects in vitro. The underlying mechanisms have not been fully elucidated although involvement of increased cellular reactive oxygen species (ROS) and DNA-damage has suggested. In this vitro study we the impact a commonly used methacrylate monomer, HEMA, on level oxidation state glutathione, intracellular ROS level, as well formation complex between HEMA glutathione. exposure rapidly led...

We have characterized cell death in THP-1 cells after exposure to heat-treated spores from satratoxin G–producing Stachybotrys chartarum isolate IBT 9631, atranone-producing S. 9634, and sterigmatocystin-producing Aspergillus versicolor 3781, as well the trichothecenes T-2 G. Spores induced within 3–6 h, with appearing most potent. 9631 both apoptosis necrosis, while 9634 3781 mostly necrosis. toxin G caused mainly apoptosis. Comet assay ± formamidopyrimidine DNA glycosylase showed that only...

2-Hydroxyethyl methacrylate (HEMA) is a monomer used in polymer-based dental-restorative materials. In this study, the viability of human lung epithelial cells, BEAS-2B, was investigated after exposure to monomer. Exposure HEMA reduced BEAS-2B cells as result increased apoptosis, interruption cell cycle, and decreased proliferation. Depletion cellular glutathione levels reactive oxygen species (ROS) were seen HEMA. The synthase inhibitor, L-buthioninesulfoximine (BSO), study whether caused...

Bronchiolar Clara cells and alveolar type 2 of the lung are known to express relatively high levels P450 enzymes compared other pulmonary cells. Populations enriched were isolated from rat by a procedure including perfusion, protease digestion, centrifugal elutriation, differential attachment. Alveolar macrophages removed lavage. The purity cell-enriched population was approximately 90%, 40-50%. Both proliferated in culture. CYP2B1 mRNA expressed same level population. remained roughly...

The tobacco alkaloid nicotine is known for its activation of neuronal nicotinic acetylcholine receptors. Nicotine consumed in different ways such as through conventional smoking, e-cigarettes, snuff or pouches. use has been associated with several adverse health effects, inflammatory reactions the oral mucosa and cavity cancer. We performed a metabolomic analysis nicotine-exposed THP-1 human monocytes. Cells were exposed to 5 mM up 4 h, cell extracts medium subjected untargeted liquid...

Abstract Objectives Material chemistry and workflow variables associated with the fabrication of dental devices may affect biocompatibility devices. The purpose this study was to compare digital conventional procedures in manufacturing acrylic-based occlusal by assessing cytotoxic potential leakage products. Methods Specimens were manufactured 3D printing (stereolithography light processing), milling, autopolymerization. Print specimens also subjected different post-curing methods. To assess...

Objective The aims of this in vitro study were to assess if dynamic loading increases the metal ion release selected dental alloys and evaluate cytotoxicity released ions.Materials methods One Pd–Ag alloy (Aurolite 2B) two Co–Cr (Wirobond 280 d.Sign 30) investigated. Two different corrosion immersion tests used: a standardized static test (ISO 22674: 2016) an experimental test. Both involved specimens lactic acidic solution (pH = 2.3). Inductively coupled plasma mass spectrometry was used...

Although dental composites are in extensive use today, little is known about the biological effects of filler particles. As composite materials gradually broken down aggressive environment oral cavity, particles may leak and induce toxic on surrounding tissue cells. The aim this study was to elucidate possible adverse commonly used particles; bariumaluminiumsilica (BaAlSi) bariumaluminiumfluorosilica (BaAlFSi) with mean size 1 microm. BEAS-2B cells were as a model system. Particle...

Several in vitro studies utilizing 2-dimensional (2D) cell culture systems have linked 2-hydroxyethyl methacrylate (HEMA) with cytotoxic effects oral mucosa and dental pulp cells. Although such are invaluable dissecting the cellular molecular of HEMA, there is a growing interest utilization appropriate 3-dimensional (3D) models that mimic structure mucosa. Using previously characterized 3D-organotypic co-culture model, this study aimed to investigate HEMA on 3D-co-culture model consisting...

Abstract Resin‐based biomaterials are widely used in medical and dental treatment, both clinicians patients exposed to the materials. The knowledge of toxicity is mainly based on vitro studies at exposure concentrations that induce cell death. However, severe damage death signaling may overshadow essential cellular events caused by a possible toxicant. For resins, interaction with living cells more clinical relevant doses sparse. 2‐Hydroxyethylmethacrylate (HEMA) commonly monomer resins....