A5044762023- Prion Diseases and Protein Misfolding
- Analytical chemistry methods development
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Neurological diseases and metabolism
- Hemoglobin structure and function
- Monoclonal and Polyclonal Antibodies Research
- Polymer Surface Interaction Studies
- Electrochemical sensors and biosensors
- Enzyme Structure and Function
- Supramolecular Self-Assembly in Materials
- Heme Oxygenase-1 and Carbon Monoxide
- Gold and Silver Nanoparticles Synthesis and Applications
- Trace Elements in Health
- Microfluidic and Capillary Electrophoresis Applications
- Peptidase Inhibition and Analysis
- Advanced Biosensing Techniques and Applications
- Pharmacological Effects and Assays
- Mass Spectrometry Techniques and Applications
- Nanocluster Synthesis and Applications
- Biochemical Analysis and Sensing Techniques
- Chemical Synthesis and Analysis
- Immunotherapy and Immune Responses
- Molecular Sensors and Ion Detection
- X-ray Diffraction in Crystallography
University of Florence
2018-2025
Data Management (Italy)
2024
Istituto Nazionale Biostrutture e Biosistemi
2015-2017
FORTH Institute of Molecular Biology and Biotechnology
2017
University Hospital of Heraklion
2017
University of Catania
2015-2016
Consortium GARR
2015
University of Minho
2015
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2011-2012
University of Reading
2012
Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics. In particular, streptavidin-modified AuNPs are increasingly used for biosensing purposes. We report here study of surface-functionalized with streptavidin-biotinylated oligonucleotide, focussing on the role played by oligonucleotide probes stabilization/destabilization functionalized nanoparticle...
We have recently investigated the aggregation behavior of zwitterionic n-dodecyl phosphocholine in presence high salt. As double logarithmic Corrin–Harkins plots critical micellar concentration versus salt were not linear, here we re-examine those data context binding model surfactant aggregation, as previously developed by us for ionic surfactants. also re-examined plenty available literature on salt-dependent neutral The use double-logarithmic allowed to show that is general applicability....
A novel method for cleaving from resin and removing acid-labile protecting groups the Fmoc solid-phase peptide synthesis is described. 0.1 N HCl in hexafluoroisopropanol or trifluoroethanol cleanly rapidly removes tert-butyl ester ether, Boc, trityl, Pbf cleaves common linkers: Wang, HMPA, Rink amide, PAL. Addition of just 5–10% a hydrogen-bonding solvent considerably retards even fully inhibits reaction. However, non-hydrogen-bonding tolerated.
Peptide materials based on the aggregation of polyphenylalanine conjugates containing gadolinium complexes and acting as potential contrast agents (CAs) in magnetic resonance imaging (MRI) are described. Monomers contain two (F2) or four (F4) phenylalanine residues for self‐assembly, a chelating agent, 1,4,7,10‐tetraazacyclododecane‐ N , ‐tetraacetic acid (DOTA) diethylenetriaminepentaacetic (DTPA), achieving coordination, ethoxylic linkers at (L 2 ) six 6 poly(ethylene glycol) (PEG) units...
Nanomaterials have revolutionized the design of detection strategies, and nowadays nanoparticles are extensively employed in innovative assays for selective sensitive a large variety analytes. Recently, new nanomaterials category, namely nanoclusters (NCs), is rapidly emerging. These nanostructures offer great advantages terms stability ease fabrication. The increasing interest NCs applications, well represented by wide bibliography reporting on gold silver NCs, opens perspectives copper...
The genome of Populus trichocarpa contains five methionine sulfoxide reductase A genes. Here, both cytosolic (cMsrA) and plastidial (pMsrA) poplar MsrAs were analyzed. two recombinant enzymes are active in the reduction with either dithiothreitol or thioredoxin as a reductant. In enzymes, cysteines, at positions 46, 81, 100, 196, 202, conserved. Biochemical enzymatic analyses cysteine-mutated support catalytic mechanism involving three cysteines 202. Cys46 is cysteine, C-terminal Cys196...
Hepatocellular carcinoma (HCC) is among the most aggressive and fatal cancers. Its treatment with conventional chemotherapeutic agents inefficient, due to several side effects linked impaired organ function typical of liver diseases. Consequently, there exists a decisive requirement explore possible alternative chemopreventive therapeutic strategies. The use dietary antioxidants micronutrients has been proposed for HCC successful management. aim this work was test in vitro lipoic acid,...
Abstract An original biomimetic enzyme-linked immunoassay (BELISA) to target the small peptide hormone gonadorelin is presented. This has been recently listed among substances banned in sports by World Antidoping Agency (WADA) since its misuse male athletes triggers testosterone increase. Hence, response this emerging issue anti-doping controls, we proposed BELISA which involves growth of a polynorepinephrine (PNE)–based molecularly imprinted polymer (MIP) directly on microwells. PNE,...
Molecular imprinting and related technologies are becoming increasingly appreciated in bioanalysis diagnostic applications. Among the imprinted polymers, we have already demonstrated that endogenous neurotransmitters (NTs) dopamine (DA) norepinephrine (NE) can be efficiently used as natural sustainable monomers to straightforwardly design synthesize a new generation of green "soft" Molecularly Imprinted BioPolymers (MIBPs). Here, for first time ability further NT, i.e., serotonin (SE),...
A colorimetric assay was developed for detection of indole-3-carbaldehyde (I3A), a bioactive compound particularly abundant in vegetables the Brassicaceae family, inducing plant defense responses and ensuring human intestinal barrier integrity. The is based on selective condensation reaction between hydrazine group carbidopa, used as derivatizing agent, aldehyde I3A, generating colored aldazine with characteristic absorbance around 415 nm. As proof concept, among several natural sources here...
By using Fourier transform infrared photolysis difference spectroscopy combined with temperature derivative at cryogenic temperatures, we have measured spectra of the stretching absorption on nitric oxide (NO) in heme-bound and photodissociated states ferrous ferric nitrosyl myoglobin (MbNO) a few site-specific Mb mutants. The NO was utilized as sensitive local probe ligand interactions active-site residues movements within protein. comparison results obtained previous spectroscopic...
Studying peptide amphiphiles (PAs), we investigate the influence of alkyl chain length on aggregation behavior collagen-derived KTTKS with applications ranging from antiwrinkle cosmetic creams to potential uses in regenerative medicine. We have studied synthetic peptides C(14)-KTTKS (myristoyl-Lys-Thr-Thr-Lys-Ser) and C(18)-KTTKS (stearoyl-Lys-Thr-Thr-Lys-Ser) detail their physicochemical properties. It is presumed that hydrophobic these self-assembling enhances permeation across skin...