A5075798675- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Assisted Reproductive Technology and Twin Pregnancy
- Renal and related cancers
- Reproductive Health and Technologies
- Mesenchymal stem cell research
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Food Supply Chain Traceability
- Animal health and immunology
- Prenatal Screening and Diagnostics
- Ruminant Nutrition and Digestive Physiology
- Birth, Development, and Health
- Bioenergy crop production and management
- Effects of Environmental Stressors on Livestock
- IoT Networks and Protocols
- Diet and metabolism studies
- Regulation of Appetite and Obesity
- Xenotransplantation and immune response
- Fatty Acid Research and Health
- Organ and Tissue Transplantation Research
Utsunomiya University
2016-2025
Tokyo University of Agriculture and Technology
2015-2025
Ono Clinic
2021-2023
Farm Foundation
2010
Megmilk Snow Brand (Japan)
1990-2007
Dankook University
2004
Chugoku Electric Power (Japan)
2001
Hokkaido University
1995
In cattle, maternal metabolic health has been suggested to influence oocyte and embryo quality. Here, we examined whether liver abnormalities affected
Abstract The objective here was to review the effects of polyvinylpyrrolidone (PVP) upon sperm function and embryonic development in humans. PVP has been used successfully intracytoplasmic injection (ICSI) facilitate handling immobilization for both domestic animals In our previous reports, solution exists locally embryos injected during early developmental period, also exerts influence over capacity such embryos. other causes significant damage membranes that can be detected by transmission...
Various factors such as embryo density and substances in the medium can influence development vitro. These embryos probably interact with each other, however interactions are not fully understood. To investigate interactions, we examined effects of number embryos, drop size, oxygen concentration glucose inorganic phosphate during protein-free culture bovine IVM/IVF embryos. In Experiment 1, different numbers were cultured a 50 microl medium. The frequencies blastocyst groups 25, 100 per...
The generation of mammalian embryos by in vitro culture is hampered the failure many to develop blastocyst stage. This problem occurs even when cumulus-oocyte complexes (COCs) with good morphology are visually selected and used for culture. Because cumulus cells important oocyte maturation subsequent embryo development, here we compared gene expression patterns COCs that developed stage those failed develop. Cumulus were aspirated from bovine Oocyte developmental competence was evaluated...
Intracytoplasmic sperm injection (ICSI) has become the method of choice to treat human male infertility. One outstanding problems associated with this technique is our current lack knowledge concerning effect capacitation and motility upon subsequent development oocytes following ICSI. In present study, we first examined state exhibiting normal motility, along that had been activated, reactive oxygen species (ROS) produced by these types embryogenesis bovine in vitro fertilization (IVF) Data...
The maturation rate of canine oocytes during in vitro (IVM) needs to be improved. present study was designed evaluate the effects insulin-like growth factor-1 (IGF-1) on IVM oocytes. Ovaries were obtained by ovariohysterectomy and sliced release cumulus-oocyte complexes (COCs). In Experiment 1, different concentrations IGF-1 nuclear investigated. COCs cultured a modified medium (mTCM199) with (0, 0.5, 5, 10, 50 µg/ml). At end 48 h culture, fixed stained their stage. Supplementation µg/ml...
Here, we investigated the effect of liver abnormality on developmental potential bovine oocytes. Good quality oocytes from healthy cows and animals with a were matured fertilized in vitro then cultured to blastocyst stage. On day 7 after fertilization, embryo cleavage development assessed. The concentrations glucose, nonesterified fatty acid (NEFA), γ-glutamyl transpeptidase (γ-GTP), β-hydroxybutyrate (BHBA) glutathione measured follicular fluids (FF). proportion good frequency stage lower...
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. samples cooled 4 C then frozen liquid nitrogen vapor. Motility parameters sperm evaluated at 0, 1, 2, 3, 4, 12 24 h after thawing. Sperm...
Background. Although directed differentiation of human embryonic stem (ES) cells would enable a ready supply and tissues required for transplantation therapy, the methodology is limited. We have developed novel method hematopoietic development from primate ES cells. first cultured cynomolgus monkey in vitro transplanted vivo into fetal sheep liver, generating with hematopoiesis. Methods. Cynomolgus were induced to mesodermal on murine stromal OP9 multiple cytokines 6 days. The (average...
The expression of mRNA leptin, the product obese gene, in bovine adipose tissue was analyzed by a lysate RNase protection assay. level significantly decreased food deprivation for two days and partially recovered after 3 hr refeeding, indicating that gene ruminant regulated feeding.
Summary The overnight preservation of bovine ovaries would be highly useful in the subsequent harvest viable oocytes for reproductive study. present study aimed to optimize conditions by examining effects temperature, solution and supplementation. In Experiment 1, rate development blastocyst stage derived from preserved at 15°C was higher than that either 5 or 25°C ( p < 0.05). 2, University Wisconsin when PBS saline used 3, supplemented with 0.3 mM glutathione (GSH) exhibited an increase...
Mammalian sperm undergo a series of biochemical transformations in the female reproductive tract that are collectively known as capacitation. One key processes involved capacitation is activation motility. Here, we investigated and fertility status activated which had been cultured media containing methyl-β-cyclodextrin (MBCD). In order to do this, single were caught using micropipette stained with chlortetracycline (CTC). Firstly, effects preincubation upon motility, fertility. Culture...