A5085338545- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Ovarian function and disorders
- Renal and related cancers
- Animal Genetics and Reproduction
- Fatty Acid Research and Health
- Xenotransplantation and immune response
- Regulation of Appetite and Obesity
- Maternal and Perinatal Health Interventions
- Assisted Reproductive Technology and Twin Pregnancy
- MicroRNA in disease regulation
- Meat and Animal Product Quality
- Animal health and immunology
- Healthcare and Venom Research
- Birth, Development, and Health
- Growth Hormone and Insulin-like Growth Factors
- Reproductive System and Pregnancy
- Animal Behavior and Welfare Studies
- Effects of Environmental Stressors on Livestock
- Zebrafish Biomedical Research Applications
- Odor and Emission Control Technologies
- Pregnancy and Medication Impact
- CRISPR and Genetic Engineering
Utsunomiya University
2012-2024
Tokyo University of Agriculture and Technology
2012
The maturation rate of canine oocytes during in vitro (IVM) needs to be improved. present study was designed evaluate the effects insulin-like growth factor-1 (IGF-1) on IVM oocytes. Ovaries were obtained by ovariohysterectomy and sliced release cumulus-oocyte complexes (COCs). In Experiment 1, different concentrations IGF-1 nuclear investigated. COCs cultured a modified medium (mTCM199) with (0, 0.5, 5, 10, 50 µg/ml). At end 48 h culture, fixed stained their stage. Supplementation µg/ml...
Here, we investigated the effect of liver abnormality on developmental potential bovine oocytes. Good quality oocytes from healthy cows and animals with a were matured fertilized in vitro then cultured to blastocyst stage. On day 7 after fertilization, embryo cleavage development assessed. The concentrations glucose, nonesterified fatty acid (NEFA), γ-glutamyl transpeptidase (γ-GTP), β-hydroxybutyrate (BHBA) glutathione measured follicular fluids (FF). proportion good frequency stage lower...
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. samples cooled 4 C then frozen liquid nitrogen vapor. Motility parameters sperm evaluated at 0, 1, 2, 3, 4, 12 24 h after thawing. Sperm...
Abstract Sheep are typically considered as industrial animals that provide wool and meals. However, they play a significant role in medical research addition to their conventional use. Notably, sheep fetuses resistant surgical invasions can endure numerous manipulations, such needle puncture cell transplantation, operations requiring exposure beyond the uterus. Based on these distinguishing characteristics, we established chimeric model capable of producing human/monkey pluripotent...
We investigated the effects of a calf starter supplemented with calcium salts medium-chain fatty acids (MCFA-Ca) on growth and plasma hormone concentration in calves. Twelve Holstein calves were randomly assigned to two dietary groups (without supplementation [CON] MCFA-Ca [MCFA]) from 4 d age. Calves fed 1.0 kg/d milk replacer until 5 wk age completely weaned at 7 MCFA group received containing 1% MCFA-Ca. dry matter intake (DMI) was measured daily, body weight weekly. Rumen fluid collected...
Objective The objective of this study was to examine the effect using a tunnel ventilation system within dairy barn environment upon productivity cows during winter season. Methods performed at University Farm, Faculty Agriculture, Utsunomiya University. Twenty-one Holstein (5 heifers and 16 multiparous) were enclosed in stall barn. Unventilated (UV) tunnel-ventilated (TV) operated by turns every other week, number key parameters measured barn, including output, temperature, relative...
•Fetal liver provides a microenvironment in which to turn iPSC derivatives into hematopoietic cells.•Human iPSC-derived hematopoiesis and multilineage progeny develop sheep.•iPSC-derived cells move from fetal bone marrow as naturally occurs•Human blood are enriched by xenogeneic donor-lymphocyte infusion sheep. We report that sheep for generating with long-term engrafting capacity differentiation potential human induced pluripotent stem cell (iPSC)–derived hemogenic endothelial (HEs)....
The aim of this study was to investigate how intraduodenal infusions fatty acids (FA) affect appetite-related gut peptides such as glucagon-like peptide-1 (GLP-1) and ghrelin in sheep. We hypothesized that these can be highly reactive unsaturated long-chain FA, because they are well known decrease dry matter intake (DMI). Four ewes were fitted with a duodenal cannula jugular vein catheter for 6-h infusion the 9 FA (C8:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1, C18:2, C18:3) water...
Although in vitro maturation (IVM) of oocytes has been successfully established for many species, the efficiency IVM canine is still very low. As growth factors have shown to promote oocyte some we investigated whether use transforming factor α (TGF-a) and insulin-like 1 (IGF-1) might overcome difficulties achieving meiotic cultured cumulus-oocyte complexes (COC). Ovaries were obtained from bitches at 6 months 7 years age by ovariohysterectomy sliced repeatedly release COC. In first...
Previously, we generated monkey/sheep haematopoietic chimeras by in utero transplantation (IUT) of monkey embryonic stem (ES); however, the factors that control how ES cells successfully engraft and differentiate into tissue sheep fetuses remain uncertain. Here, examined might influence donor recipient affect successful cell engraftment. We transplanted either undifferentiated or ES-derived at an early differentiation stage fetuses. The latter were allowed to culturing on OP9 layers for 6...
Trans-cervical insemination (TCI) with cryopreserved semen offers a potentially effective approach for breeding canids specific genetic traits, such as guide dogs the blind. However, there are technical difficulties in canine sperm cryopreservation, composition of extender. The aim this study was to evaluate effects glutathione (GSH) an antioxidant extender improve quality frozen-thawed dog sperm. A Tris-egg yolk-citrate containing 15.7 mg mL–1 TRIS, 8.8 citric acid, 14.1 lactose, 25.4...
Intracytoplasmic sperm injection (ICSI) has become the method of choice for bovine ovum pick-up and IVF. However, there are many difficulties with ICSI technique to obtain viable fetuses. One major problems associated this is our lack knowledge concerning status mitochondria when injected into oocyte its effect on embryo development. First, we examined mitochondrial activity that had been activated by culturing methyl-β cyclodextrin (MBCD), in In vitro-matured oocytes JC1-labelled were used...