A5004725679- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Reproductive Health and Technologies
- Microfluidic and Capillary Electrophoresis Applications
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Fatty Acid Research and Health
- Healthcare and Venom Research
- Regulation of Appetite and Obesity
- Milk Quality and Mastitis in Dairy Cows
- Xenotransplantation and immune response
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Seed Germination and Physiology
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Diet and metabolism studies
- Cancer-related molecular mechanisms research
Institute of Livestock and Grassland Science
2021-2025
Utsunomiya University
2012-2025
Tokyo University of Agriculture and Technology
2012-2025
National Agriculture and Food Research Organization
2020-2025
Central Region Agricultural Research Center
2023
National Livestock Breeding Center
2017-2020
Osaka Institute of Technology
2006
Significance Iatrogenic failures of assisted reproduction technology could be associated with routine sperm preparation techniques. Limitations conventional selection methods include the inability to efficiently sort functional spermatozoa and assess fertilization potential. We developed a robust microfluidic sorting system by using diffuser-type sorter device capable ultrahigh-throughput separation motile, DNA-intact, functionally competent sperm. The strategy inclusively targeted intrinsic...
The application of cryopreservation and artificial insemination technology have contributed to the advancement animal reproduction. However, a substantial proportion spermatozoa undergoes alterations loses their fertility during cryopreservation, rendering frozen-thawed semen impractical for routine use. Cryopreservation is known reduce sperm lifespan fertility. Variation in cryosurvival from different sires even with individual sire common (AI) centers. Our goal improve post-thawed quality...
The maturation rate of canine oocytes during in vitro (IVM) needs to be improved. present study was designed evaluate the effects insulin-like growth factor-1 (IGF-1) on IVM oocytes. Ovaries were obtained by ovariohysterectomy and sliced release cumulus-oocyte complexes (COCs). In Experiment 1, different concentrations IGF-1 nuclear investigated. COCs cultured a modified medium (mTCM199) with (0, 0.5, 5, 10, 50 µg/ml). At end 48 h culture, fixed stained their stage. Supplementation µg/ml...
Here, we investigated the effect of liver abnormality on developmental potential bovine oocytes. Good quality oocytes from healthy cows and animals with a were matured fertilized in vitro then cultured to blastocyst stage. On day 7 after fertilization, embryo cleavage development assessed. The concentrations glucose, nonesterified fatty acid (NEFA), γ-glutamyl transpeptidase (γ-GTP), β-hydroxybutyrate (BHBA) glutathione measured follicular fluids (FF). proportion good frequency stage lower...
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. samples cooled 4 C then frozen liquid nitrogen vapor. Motility parameters sperm evaluated at 0, 1, 2, 3, 4, 12 24 h after thawing. Sperm...
For semen suppliers, predicting the low fertility of service bull candidates before artificial insemination would help prevent economic loss; however, through in vitro assessment is yet to be established. In present study, we focused on methylated CpG sites sperm nuclear DNA and examined methylation levels screen new biomarkers for fertility. frozen-thawed samples collected from Japanese Black bulls, which sire conception rate (SCR) was recorded, level each site analyzed using human...
In order to obtain more information on the development of bovine and ovine fetal mammary glands, a series glands from fetuses different ages were analyzed. A total 16 with curved crown rump lengths ranging 12 cm (80 days) 75 (240 15 55 days 131 examined. We used hematoxylin eosin stain Oil-Red-O analyze developmental morphogenetic processes glands. addition, we immunohistochemical staining determine pattern expression cytokeratin 18 (CK18) during luminal epithelial differentiation,...
Abstract Background The isolation and characterization of sperm subpopulations that can achieve fertilization is a major challenge assisted reproduction methods. We focused on the microfluidic sorter as novel tool for collecting highly motile spermatozoa from heterogeneous semen samples. Objectives This study primarily aims to obtain baseline information sorted according its characteristics in vitro life span. Materials Methods Frozen‐thawed bull was subjected sorting using diffuser‐type...
During cryopreservation, spermatozoa may suffer cold and cryo-induced injuries -associated with alterations in cell defense systems- that are detrimental to their function subsequent fertility. This study aimed determine the efficacy of supplementing semen freezing extender antioxidant reduced glutathione (GSH) cattle. Semen was collected from four bulls diluted a supplemented or without GSH (0, 1, 5, 10 mM) before cooling step cryopreservation process. After thawing, quality frozen-thawed...
The aim of this study was to investigate how intraduodenal infusions fatty acids (FA) affect appetite-related gut peptides such as glucagon-like peptide-1 (GLP-1) and ghrelin in sheep. We hypothesized that these can be highly reactive unsaturated long-chain FA, because they are well known decrease dry matter intake (DMI). Four ewes were fitted with a duodenal cannula jugular vein catheter for 6-h infusion the 9 FA (C8:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1, C18:2, C18:3) water...
Although in vitro maturation (IVM) of oocytes has been successfully established for many species, the efficiency IVM canine is still very low. As growth factors have shown to promote oocyte some we investigated whether use transforming factor α (TGF-a) and insulin-like 1 (IGF-1) might overcome difficulties achieving meiotic cultured cumulus-oocyte complexes (COC). Ovaries were obtained from bitches at 6 months 7 years age by ovariohysterectomy sliced repeatedly release COC. In first...
Previously, we generated monkey/sheep haematopoietic chimeras by in utero transplantation (IUT) of monkey embryonic stem (ES); however, the factors that control how ES cells successfully engraft and differentiate into tissue sheep fetuses remain uncertain. Here, examined might influence donor recipient affect successful cell engraftment. We transplanted either undifferentiated or ES-derived at an early differentiation stage fetuses. The latter were allowed to culturing on OP9 layers for 6...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife
Trans-cervical insemination (TCI) with cryopreserved semen offers a potentially effective approach for breeding canids specific genetic traits, such as guide dogs the blind. However, there are technical difficulties in canine sperm cryopreservation, composition of extender. The aim this study was to evaluate effects glutathione (GSH) an antioxidant extender improve quality frozen-thawed dog sperm. A Tris-egg yolk-citrate containing 15.7 mg mL–1 TRIS, 8.8 citric acid, 14.1 lactose, 25.4...
Intracytoplasmic sperm injection (ICSI) has become the method of choice for bovine ovum pick-up and IVF. However, there are many difficulties with ICSI technique to obtain viable fetuses. One major problems associated this is our lack knowledge concerning status mitochondria when injected into oocyte its effect on embryo development. First, we examined mitochondrial activity that had been activated by culturing methyl-β cyclodextrin (MBCD), in In vitro-matured oocytes JC1-labelled were used...
In vitro-produced (IVP) embryos are more easily damaged by cryopreservation than in vivo-derived embryos. Therefore, transportation of fresh IVP a manner that can maintain viability is necessary. This study was conducted to determine the preferable culture conditions for transport at 5 days post-insemination (dpi) 1.5-mL microtubes. Cumulus-oocyte complexes derived from an abattoir were matured and then inseminated with frozen-thawed semen. Presumptive zygotes cultured mCR1aa (CR1)+5% calf...
Bio applications of compound semiconductors are great interest and much attention has been paid to ZnO-based materials because their multifunctional properties. Toward the biologically sensitive field effect transistor (BioFET) (Schoning Poghossian, 2002), biochemical functionalization remains a fundamental issue. For instance, for realization glucose sensor, specifically enzymes, such as oxidase, need be immobilized via spacer molecules. In this work, molecule modified ZnO surface well...