A5009753214- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epilepsy research and treatment
- Blood disorders and treatments
- Erythrocyte Function and Pathophysiology
- Cancer, Hypoxia, and Metabolism
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- Nuclear Structure and Function
- Immunodeficiency and Autoimmune Disorders
- Photoreceptor and optogenetics research
- Biomedical Ethics and Regulation
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cytokine Signaling Pathways and Interactions
- Protein Degradation and Inhibitors
- Neurogenesis and neuroplasticity mechanisms
- Phosphodiesterase function and regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Genetics and Neurodevelopmental Disorders
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
Mossakowski Medical Research Institute, Polish Academy of Sciences
2011-2024
Polish Academy of Sciences
2011-2024
Hebrew University of Jerusalem
2013
The Maria Sklodowska-Curie National Research Institute of Oncology
2011
Highlights•Gata3, Eomes, Tfap2c, Myc, and Esrrb convert fibroblasts into iPSCs, iTSCs, iXENs•Esrrb, but not other pluripotency genes, can shift the TSC fate pluripotency•Esrrb induces by activation of a unique XEN-like state•The interplay between Eomes determines cell decisionSummaryFollowing fertilization, totipotent cells undergo asymmetric divisions, resulting in three distinct types late pre-implantation blastocyst: epiblast (Epi), primitive endoderm (PrE), trophectoderm (TE). Here, we...
Human trophoblast stem cells (hTSCs) can be derived from embryonic (hESCs) or induced somatic by OCT4, SOX2, KLF4 and MYC (OSKM). Here we explore whether the hTSC state independently of pluripotency, what are mechanisms underlying its acquisition. We identify GATA3, (GOKM) as a combination factors that generate functional hiTSCs fibroblasts. Transcriptomic analysis stable GOKM- OSKM-hiTSCs reveals 94 hTSC-specific genes aberrant specifically in OSKM-derived hiTSCs. Through...
Dravet syndrome (DRVT) is a rare form of neurodevelopmental disorder with high risk sudden unexpected death in epilepsy (SUDEP), caused mainly (>80% cases) by mutations the SCN1A gene, coding Nav1.1 protein (alfa-subunit voltage-sensitive sodium channel). Mutations are linked to heterogenous epileptic phenotypes various types, severity, and patient prognosis. Here we generated iPSC lines from fibroblasts obtained three individuals affected DRVT carrying distinct gene (nonsense mutation...
HAX-1 is a multi-functional protein that involved in the regulation of apoptosis, cell motility and calcium homeostasis. It also reported to bind RNA: it associates with structural motifs present 3' untranslated regions at least two transcripts, but functional significance this binding remains unknown. Although has been detected various cellular compartments, predominantly cytoplasmic. Our detailed localization studies isoforms revealed partial nuclear localization, extent which depends on...
Standardization of methods for obtaining iPS cells from the human somatic and then their successful differentiation are important in context possible application personalized cell therapy development toxicological pharmacological tests. In present study, influence small molecules representing epigenetic modulators (histone deacetylase inhibitor Trichostatin A DNA methyltransferase RG-108) on process reverting neural progenitors HUCB-NSC (Human Umbilical Cord Blood Neural Stem Cell) line to...
HAX-1, a multifunctional protein involved in the regulation of apoptosis, cell migration, and calcium homeostasis, binds 3′ untranslated region motifs specific transcripts. This suggests that HAX-1 plays role post-transcriptional regulation, at level mRNA stability/transport or translation. In this study, we analyze detail colocalization with processing bodies (P-bodies) its dependence on availability. Endogenous P-body markers DCP1 Rck/p54 were shown to colocalize endogenous but case...
In the context of cell therapy, epigenetic status core stemness transcription factor (STF) genes regulating proliferation/differentiation program is primary interest. Our results confirmed that in vitro differentiation umbilicalcord-blood-derived-neural-stem-cells (HUCB-NSC) coincides with progressive down-regulation Oct3/4 and Nanog gene expression. Consistently parallel repression transcription, a substantial increase mosaic cytosine methylation CpG dinucleotide was observed promoter...
AMA Kuczynska Z, Neglur P, Metin E, et al. Safety of GMP-compliant iPSC lines generated by Sendai virus transduction is dependent upon clone identity and sex the donor. Folia Neuropathologica. 2024;62(1):32-46. doi:10.5114/fn.2024.134026. APA Kuczynska, Z., Neglur, P., Metin, E., Liput, M., Zychowicz, & Zayat, V. (2024). Neuropathologica, 62(1), 32-46. https://doi.org/10.5114/fn.2024.134026 Chicago Zuzanna, Pawan Kumar Erkan Michal Marzena Valery Natalia E. Krześniak 2024. "Safety donor"....
SUMMARY Recent studies demonstrated that human trophoblast stem-like cells (hTS-like cells) can be derived from naïve embryonic stem or induced somatic by the pluripotency factors, OSKM. This raises two main questions; (i) whether TSCs (hiTSCs) generated independently to pluripotent state factors and (ii) what are mechanisms which hTSC is established during reprogramming. Here, we identify GATA3, OCT4, KLF4 MYC (GOKM) as a pluripotency-independent combination of generate stable functional...