A5048193191- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Epilepsy research and treatment
- Gene Regulatory Network Analysis
- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Cancer, Lipids, and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- stochastic dynamics and bifurcation
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Neonatal Respiratory Health Research
- Genetic and phenotypic traits in livestock
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Biomedical Ethics and Regulation
- Microtubule and mitosis dynamics
University at Buffalo, State University of New York
2020-2024
Mossakowski Medical Research Institute, Polish Academy of Sciences
2020-2024
Polish Academy of Sciences
2020-2024
West Pomeranian University of Technology
2019
Dravet syndrome (DRVT) is a rare form of neurodevelopmental disorder with high risk sudden unexpected death in epilepsy (SUDEP), caused mainly (>80% cases) by mutations the SCN1A gene, coding Nav1.1 protein (alfa-subunit voltage-sensitive sodium channel). Mutations are linked to heterogenous epileptic phenotypes various types, severity, and patient prognosis. Here we generated iPSC lines from fibroblasts obtained three individuals affected DRVT carrying distinct gene (nonsense mutation...
Schizophrenia (SZ) is a neurodevelopmental genetic disorder in which maternal immune activation (MIA) and increased tumor necrosis factor-α (TNF-α) may contribute. Previous studies using iPSC-derived cerebral organoids neuronal cells demonstrated developmental malformation transcriptional dysregulations, including TNF receptors their signaling genes, common to SZ patients with diverse backgrounds. In the present study, we examined significance of receptor dysregulations by transiently...
Polyunsaturated fatty acids (PUFAs) play a role in wide variety of physiological processes. They are produced by series desaturation and elongation reactions. Δ -6-desaturase is membrane-bound enzyme that catalyzes the conversion α -linolenic acid (C18:3 n -3) linoleic (C18:2 -6) to stearidonic (18:4 γ (18:3 -6). It encoded FADS2 gene located on bovine chromosome 29. The aim this study was identify single nucleotide polymorphism determine possible associations with milk composition two...
During the development of mouse embryonic stem cells (ESC) to neuronal committed (NCC), coordinated changes in expression 2851 genes take place, mediated by nuclear form FGFR1. In this paper, widespread differences are demonstrated ESC and NCC inter- intra-chromosomal interactions, chromatin looping, formation CTCF- nFGFR1-linked Topologically Associating Domains (TADs) on a genome-wide scale exemplary HoxA-D loci. The analysis centered HoxA cluster shows that blocking FGFR1 disrupts loop...
Abstract Homeostatic control of neuronal excitability by modulation synaptic inhibition (I) and excitation (E) the principal neurons is important during brain maturation. The fundamental features in-utero development, including local E–I ratio bioenergetics, can be modeled cerebral organoids (CO) that have exhibited highly regular nested oscillatory network events. Therefore, we evaluated a 'Phase Zero' clinical study platform combining broadband Vis/near-infrared(NIR) spectroscopy...
The aim of the study was to evaluate effect different types high-fat diets (HFDs) on proteomic profile mouse liver. analysis included four dietary groups mice fed a standard diet (STD group), rich in SFAs (SFA and dominated by PUFAs with linoleic acid (LA, C18:2n-6) α-linolenic (ALA, C18:3n-3) ratios 14:1 (14:1 group) 5:1 (5:1 group). After three months diets, liver proteins were resolved two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3-10 pH gradient strips. Protein spots...
AMA Kuczynska Z, Neglur P, Metin E, et al. Safety of GMP-compliant iPSC lines generated by Sendai virus transduction is dependent upon clone identity and sex the donor. Folia Neuropathologica. 2024;62(1):32-46. doi:10.5114/fn.2024.134026. APA Kuczynska, Z., Neglur, P., Metin, E., Liput, M., Zychowicz, & Zayat, V. (2024). Neuropathologica, 62(1), 32-46. https://doi.org/10.5114/fn.2024.134026 Chicago Zuzanna, Pawan Kumar Erkan Michal Marzena Valery Natalia E. Krześniak 2024. "Safety donor"....
As human progenitor cells differentiate into neurons, the activities of many genes change; these changes are maintained within a narrow range, referred to as genome homeostasis. This process, which alters synchronization entire expressed genome, is distorted in neurodevelopmental diseases such schizophrenia. The coordinated gene activity networks formed by altering sets comprise recurring coordination modules, governed entropy-controlling action nuclear FGFR1, known be associated with DNA...
Abstract Homeostatic control of neuronal excitability by modulation synaptic inhibition (I) and excitation (E) the principal neurons is important during brain maturation. The fundamental features in-utero developmental, including local E-I ratio bioenergetics, can be modeled cerebral organoids (CO) that have exhibited highly regular nested oscillatory network events. Therefore, we evaluated a ‘Phase Zero’ clinical study platform combining broadband Vis/near-infrared(NIR) spectroscopy...
The physiological origin of the aperiodic signal present in electrophysiological recordings, called l/f neural noise, is unknown; nevertheless, it has been associated with health and disease. power spectrum slope, <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$-\alpha$</tex> xmlns:xlink="http://www.w3.org/1999/xlink">$1/\mathrm{f}^{\alpha}$</tex> , postulated to be related dynamic balance between excitation (E) inhibition (I). Our study found...
Abstract The physiological origin of the aperiodic signal present in electrophysiological recordings, called 1/f neural noise, is unknown; nevertheless, it has been associated with health and disease. power spectrum slope, -a a , postulated to be related dynamic balance between excitation (E) inhibition (I). Our study found that human cerebral organoids grown from induced pluripotent stem cells (iPSCs) Schizophrenia patients (SCZ) showed structural changes altered elasticity compared normal...