A5061590626- Mesenchymal stem cell research
- Lymphatic System and Diseases
- Pluripotent Stem Cells Research
- Peripheral Nerve Disorders
- Skin and Cellular Biology Research
- Orthopedic Surgery and Rehabilitation
- Reconstructive Surgery and Microvascular Techniques
- Myofascial pain diagnosis and treatment
- Tendon Structure and Treatment
- Body Contouring and Surgery
- Wnt/β-catenin signaling in development and cancer
- Nerve Injury and Rehabilitation
- Colorectal Cancer Surgical Treatments
- Tissue Engineering and Regenerative Medicine
- Pancreatitis Pathology and Treatment
- melanin and skin pigmentation
- Dermatological and Skeletal Disorders
- Wound Healing and Treatments
- Diagnosis and Treatment of Venous Diseases
- Polish Law and Legal System
- Advancements in Transdermal Drug Delivery
- Aesthetic Perception and Analysis
- Pressure Ulcer Prevention and Management
- Anatomy and Medical Technology
- Amyotrophic Lateral Sclerosis Research
Postgraduate School of Molecular Medicine
2011-2025
Chang Gung University
2024
Chang Gung Memorial Hospital
2024
Memorial Hospital
2024
Medical University of Lublin
2023
Medical University of Warsaw
2023
University Clinical Centre
2023
Central Clinical Hospital
2023
Memorial Hospital
2015
Grochowski Hospital
2013
ABSTRACT The Charles procedure (CP) is a potentially devastating treatment; however, in cases of an end stage untreated or improperly treated lymphedema, it the ultimate surgical therapy. As life‐saving solution, quickly relieves patients with giant, hypertrophic extremities, mostly ambulation and hygiene maintenance. Nevertheless, long‐term results may disappoint both doctors patients, who struggle social stigma, need for lifelong compression, massive lymphoedema distal parts feet, badly...
Introduction: Upper limb lymphedema is the most common complication after breast cancer therapy. Suddenly disturbed lymphatic transport in affected arm causes tissue fluid accumulation spaces, enlargement, and secondary changes tissue. Early compression therapy necessary. We aim to evaluate effectiveness of intermittent pneumatic (IPC) movement along possibility creating compensatory drainage pathways. Methods Results: investigated 25 patients with Breast Cancer Related Lymphedema (BCRL)...
Currently, the number of stem-cell based experimental therapies in neurological injuries and neurodegenerative disorders has been massively increasing. Despite fact that we still have not obtained strong evidence mesenchymal stem/stromal cells' neurogenic effectiveness vivo, research may need to focus on more appropriate sources result therapeutically promising cell populations. In this study, used dedifferentiated fat cells (DFAT) are proven demonstrate pluripotent abilities comparison with...
Objective/Purpose. Evaluation of efficacy and safety autologous adipose-derived regenerative cells (ADRCs) treatment in autoimmune refractory epilepsy. Patients. Six patients with proven or probable epilepsy (2 Rasmussen encephalitis, 2 antineuronal autoantibodies serum, possible FIRES) were included the project approval Bioethics Committee.Intrathecal injection ADRC acquired through liposuction followed by enzymatic isolation was performed. The procedure repeated 3 times every months each...
Background: Carpal tunnel release is the gold standard for treatment of median nerve compression disease. Recurrent or persistent symptoms do not occur in most patients, although a small number them have indicated that such postoperative condition indeed exists. Some patients undergo repeated treatments. In majority cases, disease associated with scarring carpal even reformation ligament. The authors propose usage autologous fat grafting during secondary to inhibit process. Methods: Ten...
The development of an effective method melanocyte isolation and culture is necessary for basic clinical studies concerning skin diseases, including pigmentation disorders melanoma. In this paper, we describe a novel, non-enzymatic metastatic melanoma cell (along with the spontaneous spheroid creation) from or lymph node explants. based on selective harvesting melanocytes cells emigrating cultured Thereby, isolated retain their natural phenotypical features, such as expression tyrosinase...
Stem cells (SCs) may constitute a perspective alternative to pharmacological treatment in neurodegenerative diseases. Although the safety of SC transplantation has been widely shown, their clinical efficiency amyotrophic lateral sclerosis (ALS) is still be proved. It not only due limited number studies, small groups, and fast but nonlinear disease progression also lack objective methods able show subtle changes. Preliminary guidelines for cell therapy have recently proposed by group ALS...
Introduction: In advanced lymphedema of lower limbs, stage III bandaging under the routinely applied pressure 40–60 mmHg remains largely ineffective. This is caused by skin and subcutaneous tissue stiffness due to fibrosis. Edema fluid accumulates deep in tissue. Evacuating this requires a high external compression force overcome resistance fibrous We aimed investigate effectiveness method, with lasting for 3 days.
ABCA4 gene mutations are the cause of a spectrum retinopathies, and most common juvenile macular degeneration is called Stargardt disease. has previously been observed almost exclusively in retina. Therefore, studying functional consequences variants required advanced molecular analysis techniques. The aim present study was to evaluate whether human hair follicles may be used for splice-site patients with retinopathies. We assessed expression skin at mRNA protein levels by means real-time...
The reconstruction of nerve continuity after traumatic injury is the gold standard in hand surgery. Immediate, tension-free, end-to-end suture ensures best prognosis. recovery mostly promising; however, a few cases, insufficient outcomes motor or sensory function are observed. Intra- and extra-fascicular scarring accompanies regeneration process limits final outcomes. Secondary release those cases recommended. Unfortunately, recurrence cannot be eliminated secondary revision neurolysis....
A universal classification of the negative outcomes carpal tunnel release surgery does not, as yet, exist. In order to avoid use arbitrary factors we have applied Accordion Severity Grading System, which uses rigorously defined qualitative terms classify complications. It also provides a uniform manner for grading severity complications, enabling outcome comparisons between centres. We analysed 500 device-assisted releases performed over 2-year period in author's department. establish...
Background: Lipedema of lower limbs is characterized by bilateral accumulations excess adipose tissue starting from the ankle to hips and buttocks. The studies with lymphoscintigraphy (LSC) magnetic resonance (MR) lymphography show altered transport index enlarged lymphatic vessels (LVs). Our aimed investigate superficial lymph flow, water accumulation, skin subcutaneous elasticity, possibility using this information diagnose lipedema. Methods Results: Fifty patients lipedema 50 control...
The epidermis forms the barrier between an organism and its external environment. Although one of major functional elements is lipid-enriched extracellular matrix, containing mainly ceramides, cholesterol (CHOL) free fatty acids, data are limited regarding lipid profile in epidermis. aim study was to determine whole acids (FAs) examine any dependence according age subject site on epidermis.Epidermis extracts obtained from 10 adults 6 children were analyzed by gas chromatography-mass...
Introduction: Lymph flows along the lymphatics due to spontaneous contraction. However, injury and inflammation may deteriorate lymphatic' s endothelial muscle cells valves. In consequence, lymphatic vessels (LVs) become insufficient. Their contraction strength rate slow down, then lymph flow stops. Our study aimed investigate changes in early lymphedema cases. Methods Results: 36 patients with unilateral stages 0 I, we performed indocyanine green (ICG) lymphography, lymphoscintigraphy, skin...
Abstract The application of mesenchymal stem/stromal cells (MSC) in regenerative medicine offers hope for the effective treatment incurable or difficult‐to‐heal diseases. However, it requires development unified protocols both safe and efficient cell acquisition clinical usage. therapeutic effect fat grafts (containing stem cells) non‐healing wounds has been discussed previous studies, although local general anaesthesia. MSC derived from adipose tissue (ASC) could be a less invasive method,...
Lower limb trauma often results in mangled extremities, and some cases, complete amputation may be necessary. However, limiting the extent of preserving major knee joint are crucial to enhance mobility overall functionality. By providing painless soft tissue coverage on stump, early prosthesis use initiation physiotherapy become more feasible. Soft transfers hold potential benefit patients two essential aspects: first, resolving deficiencies without causing bone shortening, second, preparing...
<title>Abstract</title> <bold>Background</bold>: The Mendelian Disorders of Cornification (MeDOC) comprise a large number disorders that are manifested by either localised (palmoplantar keratoderma, PPK) or generalised (ichthyoses) symptoms. MeDOC highly heterogenic in terms genetics and phenotype. Consequently, diagnostic process is challenging before implementation the next generation sequencing, was mostly symptomatic, not causal, which limited research on those diseases. aim study to...
Abstract Background The Mendelian Disorders of Cornification (MeDOC) comprise a large number disorders that present with either localised (palmoplantar keratoderma, PPK) or generalised (ichthyoses) signs. MeDOC are highly heterogenic in terms genetics and phenotype. Consequently, diagnostic process is challenging before implementation the next generation sequencing, was mostly symptomatic, not causal, which limited research on those diseases. aim study to genetically characterise cohort 265...
Colon anastomosis is therapeutically challenging because multiple, usually undetectable factors influence a spectrum of repair mechanisms. We hypothesized that low molecular weight heparins, routinely administered perioperatively, may differentially affect gene expression related to colon healing. Twenty pairs untreated and enoxaparin-treated rats underwent left-side hemicolectomy with primary end-to-end anastomosis. Normal anastomotic bowel segments were resected on day 0 days 1, 3, 5, 7...
AMA Kuczynska Z, Neglur P, Metin E, et al. Safety of GMP-compliant iPSC lines generated by Sendai virus transduction is dependent upon clone identity and sex the donor. Folia Neuropathologica. 2024;62(1):32-46. doi:10.5114/fn.2024.134026. APA Kuczynska, Z., Neglur, P., Metin, E., Liput, M., Zychowicz, & Zayat, V. (2024). Neuropathologica, 62(1), 32-46. https://doi.org/10.5114/fn.2024.134026 Chicago Zuzanna, Pawan Kumar Erkan Michal Marzena Valery Natalia E. Krześniak 2024. "Safety donor"....
Abstract In the era of a constantly growing number reports on therapeutic properties dedifferentiated, ontogenetically rejuvenated cells and their use in treatment neurological diseases, optimization derivation long-term culture methods seem to be crucial. One solutions is seen dedifferentiated fat (DFATs) which are characterized by greater homogeneity. Moreover, these possess higher expression transcriptional factors necessary maintain pluripotency (STRFs) as well ability differentiate...
Ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5mC), first to 5-hydroxymethylcytosine (5hmC), then 5-formylcytosine (5fC), and finally 5-carboxycytosine (5caC). Evidence suggests that changes in TET expression may impact cell function phenotype aging. Proliferation, apoptosis, markers autophagy double-strand DNA break repair, Fibulin 5 were assessed by flow cytometry TET1 TET2-overexpressing fibroblasts isolated from sun-unexposed skin young (23-35 years)...