A5026569515- Estrogen and related hormone effects
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Heat shock proteins research
- Clusterin in disease pathology
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Aquaculture disease management and microbiota
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Physiological and biochemical adaptations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Gene Regulatory Network Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Computational Drug Discovery Methods
- Animal Genetics and Reproduction
Institut de Recherche en Santé, Environnement et Travail
2011-2023
École des Hautes Études en Santé Publique
2019-2023
Inserm
2013-2023
Université de Rennes
2001-2023
Physique des interactions ioniques et moléculaires
2005-2012
Centre National de la Recherche Scientifique
1997-2010
Institut National des Sciences Appliquées de Lyon
1999
University of Illinois Urbana-Champaign
1993-1995
Illinois College
1993-1995
Fish Physiology and Genomics Institute
1992
We have used a transient transfection system with cytomegalovirus-based vector expressing high levels of biologically active human estrogen receptor (ER) in COS-1 cells to study the phosphorylation ER and identify major hormone-regulated sites. The features wild-type were very similar those previously observed for endogenous uterine cells: exhibited basal level which was increased approximately 3-4-fold by (estradiol) antiestrogens (hydroxytamoxifen ICI164,384), an almost extent activation...
A relation between the chemical structure of a xenobiotic and its steroidal action has not yet been clearly established. Thus, it is possible to define estrogenic potency different xenobiotics. An assessment may be accomplished by use bioassays. We have previously developed yeast system highly stably expressing rainbow trout estrogen receptor (rtER) in order analyze biological activity receptor. The recombinant appears reliable, rapid sensitive bioassay for screening determination direct...
The glucocorticoid receptor (GR) is a transcription factor, subject to several types of posttranslational modifications including phosphorylation and ubiquitination. We showed that the GR covalently modified by small ubiquitin-related modifier-1 (SUMO-1) peptide in mammalian cells. demonstrated sumoylation not dependent on presence ligand regulates stability protein as well its transcriptional activity. SUMO-1 overexpression induces dramatic degradation, abolished proteasome inhibition. also...
Clusterin is a usually secreted glycoprotein with chaperone properties. Recently, it has been suggested that clusterin isoforms reside in the nuclear and cytosolic compartments of human cell types, where they can influence various cellular programs including DNA repair, transcription apoptosis. Several mechanisms have proposed to explain this atypical location, alternative initiation splicing. However, none these unequivocally established as occurring live cells. Here we provide direct...
Clusterin is a secreted protein chaperone up-regulated in several pathologies, including cancer and neurodegenerative diseases. The present study shows that accumulation of aberrant proteins, caused by the proteasome inhibitor MG132 or incorporation amino acid analogue AZC (L-azetidine-2-carboxylic acid), increased both clusterin mRNA levels human glial cell line U-251 MG. Consistently, treatment was capable stimulating 1.3 kb gene promoter. Promoter deletion mutation studies revealed...
This study was conducted to determine if the cadmium-mediated inhibition of vitellogenesis observed in fish collected from contaminated areas or undergoing experimental exposure cadmium correlated with modification transcriptional activity estrogen receptor. A recombinant yeast system expressing rainbow trout (Oncorhynchus mykiss) estradiol receptor human used evaluate direct effect on activity. In yeast, reduced estradiol-stimulated transcription an estrogen-responsive reporter gene....
We describe the isolation and characterization of a cDNA for trout prolactin (tPrl). An extensive analysis tPrl recombinant clones by restriction sequencing revealed presence only one form mRNA. The deduced protein sequence consists 210 amino acids, including signal peptide 23 acids. acid mature is compared among teleosts mammals, showing two domains strong similarity that may be involved in biological activity.
Protein transduction domains (PTDs) are promising tools for transducing presynthesized polypeptides across the plasma membrane. However, development and optimization of PTDs hampered by many technical problems artifacts resulting notably from tight binding to cell surface difficulty in discriminating, through imagery analyses, truly cytosolic cytoplasmic vesicular compartments. To circumvent these problems, we have developed an unambiguous enzymatic assay uptake PTD-driven proteins, based on...
Estrogen receptor-alpha (ERα) is the driving transcription factor in 70% of breast cancers and its activity associated with hormone dependent tumor cell proliferation survival. Given recurrence resistant relapses, understanding etiological factors fueling resistance major clinical interest. Hypoxia, a frequent feature solid microenvironment, has been described to promote endocrine by triggering ERα down-regulation both vitro vivo models. Yet, consequences hypoxia on genomic remain largely...
Chaperone synthesis in response to proteotoxic stress is dependent on a family of transcription factors named heat shock (HSFs). The two main this family, HSF1 and HSF2, are co-expressed numerous tissues where they can interact form heterotrimers proteasome inhibition. HSF2 exhibit alternative splicing isoforms, called α β, which contribute additional complexity HSF transcriptional regulation, but remain poorly examined the literature. In work, we studied activity isoforms transfected into...