A5079206458- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Reproductive biology and impacts on aquatic species
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cytokine Signaling Pathways and Interactions
- Aquaculture Nutrition and Growth
- Reproductive System and Pregnancy
- Inflammatory mediators and NSAID effects
- NF-κB Signaling Pathways
- Menopause: Health Impacts and Treatments
- Genomics and Chromatin Dynamics
- Phytoestrogen effects and research
- Molecular Biology Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- Osteoarthritis Treatment and Mechanisms
- Receptor Mechanisms and Signaling
- Gene Regulatory Network Analysis
- Pharmacogenetics and Drug Metabolism
- Nuclear Receptors and Signaling
- Melanoma and MAPK Pathways
- Cancer, Lipids, and Metabolism
Université de Rennes
2013-2024
Institut de Recherche en Santé, Environnement et Travail
2013-2024
Inserm
2014-2024
École des Hautes Études en Santé Publique
2019-2024
Centre National de la Recherche Scientifique
2004-2017
Université de Toulouse
2017
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2013-2015
Physique des interactions ioniques et moléculaires
2003-2009
Oncogenesis Stress Signaling
2008
European Molecular Biology Laboratory
1998-2002
The teleost fish are thought to lack the mineralocorticoid hormone aldosterone but possess receptor (MR) homologs. Here we describe characterization of two rainbow trout (Oncorhynchus mykiss) MRs, called rtMRa and rtMRb. open reading frame cDNA encoded a protein 1041 amino acids. rtMRb predicted sequence is similar, differing in only 10 acids nonconserved A/B domain lacking three-amino acid insertion between zinc fingers C domain. Expression rtMR mRNA (sum both forms), measured juvenile by...
ABSTRACT Rainbow trout hepatocyte primary culture was used to test the influence of some xenobiotics on expression two genes implicated in reproduction, those for estrogen receptor (ER) and vitellogenin (Vg). We showed that chlordecone, nonylphenol, a polychlorobiphenol (PCB) mixture (Aroclor 1245) lindane were able induce ER Vg mRNA accumulation. Antiestrogens, 4-hydroxytamoxifen ICI 164,384, prevented effects xenobiotics, indicating induction gene is mediated by ER. Among these four only...
The isolation and characterization of several new human estrogen receptor-alpha (hERalpha) mRNAs are described. Together with those previously identified, they give rise to a total six hERalpha mRNA isoforms (A-F mRNAs). Produced from single gene by multiple promoter usage, all these transcripts encode common protein but differ in their 5'-untranslated region as consequence alternative splicing five upstream exons (1B-1F). RT-PCR S1 nuclease mapping analysis different revealed differential...
The transcriptional activity of ER␣ (or NR3A1) after binding ligand is mediated through synergistic action between activation functions (AFs) AF-1 and AF-2 the machinery.This functionally achieved by bridging coactivators such as CEBP protein/p300 members p160 subfamily steroid receptor coactivator protein-1 (SRC-1).We previously identified a conserved potential ␣-helical structure within functional core, evaluating point mutants human (hER␣) this region, we show that in transfection...
Abstract The beneficial influence of E2 in the maintenance healthy bone is well recognized. However, way which actions this hormone are mediated less clearly understood. Western blot analysis ERα osteoblasts demonstrated that characterized 66-kDa was only one isoforms present. Here we describe a 46-kDa isoform ERα, expressed at level similar to isoform, also present human primary osteoblasts. This shorter generated by alternative splicing an gene product, results exon 1 being skipped with...
17β-Estradiol (E2) regulates estrogen receptor-α (ERα) target gene transcription through the two independent activation functions (AFs), AF1 and AF2, located in N-terminal ligand binding domain of ERα, respectively. We previously reported that ERα is required for E2 atheroprotective action as well its accelerative on endothelial healing, but function dispensable. Here, we investigated role ERαAF2 these major beneficial actions by electively targeting (named 0 ) . Our results prove four...
Full-length 66-kDa estrogen receptor alpha (ERalpha) stimulates target gene transcription through two activation functions (AFs), AF-1 in the N-terminal domain and AF-2 ligand binding domain. Another physiologically expressed 46-kDa ERalpha isoform lacks A/B domains is consequently devoid of AF-1. Previous studies cultured endothelial cells showed that might not be required for estradiol (E2)-elicited NO production. To evaluate involvement vasculoprotective actions E2, we generated a...
CXCR4 and CXCR7 are the two receptors for chemokine CXCL12, a key mediator of growth effect estrogens (E2) in estrogen receptor (ER)-positive breast cancers. In this study we examined E2-regulation CXCL12 axis components their involvement cancer cells. were differentially regulated by E2 which enhanced expression both but repressed CXCR7. Formaldehyde-associated isolation regulatory elements (FAIRE) revealed that E2-mediated transcriptional regulation these genes is linked to control...
The expression of two human estrogen receptor-alpha (hERalpha) isoforms has been characterized within receptor-alpha-positive breast cancer cell lines such as MCF7: the full-length hERalpha66 and N terminally deleted hERalpha46, which is devoid activation function (AF)-1. Although known to mediate mitogenic effects that estrogens have on MCF7 cells, exact hERalpha46 in these cells remains undefined. Here we show that, during growth, mainly expressed nucleus at relatively low levels, whereas...
ABSTRACT An aggregate culture system for rainbow trout hepatocytes was developed to study liver-specific mRNA expression. Maintenance of differentiated functions and morphology were examined using both monolayer systems. The estrogen receptor vitellogenin genes induced by estradiol their mRNAs used as markers cell differentiation during culture. In culture, established very few cell-cell contacts in minimal media. use more complete media promotes islet formation. Hepatocyte response...
Abstract Estrogen receptor-α (ERα) regulates gene transcription through the 2 activation functions (AFs) AF-1 and AF-2. The crucial role of ERαAF-2 was previously demonstrated for endometrial proliferative action 17β-estradiol (E2). Here, we investigated ERαAF-1 in regulation cell proliferation uterus. We show that acute treatment with E2 or tamoxifen, which selectively activates ERαAF-1, similarly regulate expression a uterine set estrogen-dependent genes as well epithelial uterus wild-type...
To date, all studies conducted on breast cancer diagnosis have focused the expression of full-length 66-kDa estrogen receptor alpha (ERα66). However, much less attention has been paid to a shorter 46-kDa isoform (ERα46), devoid N-terminal region containing transactivation function AF-1. Here, we investigated levels ERα46 in tumors relation tumor grade and size, examined mechanism its generation specificities coregulatory binding functional activities. Using approaches combining...
Approximately 80% of breast cancer (BC) cases express the estrogen receptor (ER), and 30–40% these acquire resistance to endocrine therapies over time. Hyperactivation Akt is one mechanisms by which acquired. Apigenin (Api), a flavone found in several plant foods, has shown beneficial effects chronic diseases. Here, we studied therapeutic potential Api treatment ER-positive, therapy-resistant BC. To achieve this objective, stably overexpressed constitutively active form protein MCF-7 cells...
In rainbow trout as well in other species, variability of estrogen receptor (ER) gene expression according to the cell type and physiological state reflects a differential sensitivity estrogen. We previously demonstrated that (rtER) vitellogenin (Vg) genes were induced differently by estrogens liver. Therefore, these two offered suitable model study influence ER concentration on transcriptional activities. present we show transcription rate rtER Vg during an estrogenic treatment are affected...
A characteristic of all estrogen receptors (ER) cloned from fish to date is the lack first 37–42 N-terminal amino acids specific domain. Here we report isolation and characterization trout ovary a full-length complementary DNA (cDNA) clone encoding an variant form rainbow ER (rtER). Sequence analysis open reading frame this cDNA predicts 622-amino acid protein. The C-terminal region protein, position 45 end, was very similar previously reported rtER (referred as short form, or rtERS). In...