A5076363167- Estrogen and related hormone effects
- Angiogenesis and VEGF in Cancer
- Cytokine Signaling Pathways and Interactions
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Reproductive System and Pregnancy
- Circadian rhythm and melatonin
- Menopause: Health Impacts and Treatments
- Nuclear Receptors and Signaling
- Fatty Acid Research and Health
- Hormonal Regulation and Hypertension
- Prostate Cancer Treatment and Research
- Dietary Effects on Health
- Advanced Breast Cancer Therapies
- HER2/EGFR in Cancer Research
- 14-3-3 protein interactions
- Antioxidant Activity and Oxidative Stress
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- Protein Kinase Regulation and GTPase Signaling
- Bee Products Chemical Analysis
- Cholesterol and Lipid Metabolism
- Lipid metabolism and biosynthesis
- Phytoestrogen effects and research
- Bone Metabolism and Diseases
Institut des Maladies Métaboliques et Cardiovasculaires
2013-2024
Inserm
2013-2024
Université Toulouse III - Paul Sabatier
2012-2024
Université de Toulouse
2013-2023
University of Pennsylvania
2017-2021
École Nationale Vétérinaire de Toulouse
2012-2017
Université de Bordeaux
2017
Centre National de la Recherche Scientifique
2017
Institut de Recherche en Santé, Environnement et Travail
2017
Université de Rennes
2017
Significance The in vivo roles of plasma membrane-associated estrogen receptor (ER)α, including cross-talk with nuclear ERα, are poorly understood. We created a mouse point mutation the palmitoylation site ERα (C451A-ERα) to obtain membrane-specific loss function. A complementary lacking activation function AF-2 (ERα-AF2 0 ) provided selective actions. Physiologic studies revealed critical requirements for membrane receptors ovarian and thereby fertility, vascular physiology. In contrast,...
The nuclear receptors REV-ERBα and -β link circadian rhythms metabolism. Like other receptors, REV-ERB activity can be regulated by ligands, including naturally occurring heme. A putative ligand, SR9009, has been reported to elicit a range of beneficial effects in healthy as well diseased animal models cell systems. However, the direct involvement REV-ERBs these SR9009 not thoroughly assessed, experiments were performed complete absence both proteins. Here, we report generation mouse model...
Estrogens directly promote the growth of breast cancers that express estrogen receptor α (ERα). However, contribution stromal expression ERα in tumor microenvironment to protumoral effects has never been explored. In this study, we evaluated molecular and cellular mechanisms by which 17β-estradiol (E2) impacts modulates development ERα-negative tumors. Using different mouse models ER-negative cancer cells grafted subcutaneously into syngeneic ovariectomized immunocompetent mice, found E2...
Ambient temperature influences the molecular clock and lipid metabolism, but impact of chronic cold exposure on circadian metabolism in thermogenic brown adipose tissue (BAT) has not been studied. Here we show that during (1 wk at 4 °C), genes controlling de novo lipogenesis (DNL) including Srebp1 , master transcriptional regulator DNL, acquired high-amplitude rhythms BAT. These conditions activated mechanistic target rapamycin 1 (mTORC1), an inducer expression, engaged repressors REV-ERBα β...
Abstract Estrogen receptor-α (ERα) regulates gene transcription through the 2 activation functions (AFs) AF-1 and AF-2. The crucial role of ERαAF-2 was previously demonstrated for endometrial proliferative action 17β-estradiol (E2). Here, we investigated ERαAF-1 in regulation cell proliferation uterus. We show that acute treatment with E2 or tamoxifen, which selectively activates ERαAF-1, similarly regulate expression a uterine set estrogen-dependent genes as well epithelial uterus wild-type...
Background Although estrogen receptor α ( ER α) acts primarily as a transcription factor, it can also elicit membrane‐initiated steroid signaling. Pharmacological tools and transgenic mouse models previously highlighted the key role of signaling in 2 actions estrogens endothelium: increase NO production acceleration reendothelialization. Methods Results Using mice with mutated at cysteine 451 (ERaC451A), recognized palmitoylation site required for plasma membrane location, disruption nuclear...
Obesity occurs when energy expenditure is outweighed by intake. Tuberal hypothalamic nuclei, including the arcuate nucleus (ARC), ventromedial (VMH), and dorsomedial (DMH), control food intake expenditure. Here we report that, in contrast with females, male mice lacking circadian nuclear receptors REV-ERBα –β tuberal hypothalamus (HDKO mice) gained excessive weight on an obesogenic high-fat diet due to both decreased increased during light phase. Moreover, rebound after fasting was markedly...
Loss of SCN REV-ERBs alters oscillator period but does not render the clock arrhythmic.
The main estrogen, estradiol (E2), exerts several beneficial vascular actions through estrogen receptor (ER)α in endothelial cells. However, the impact of other natural estrogens such as estriol (E3) and estetrol (E4) on arteries remains poorly described. In present study, we reported effects E3 E4 healing after carotid artery injuries vivo. After endovascular injury, that preserves smooth muscle cells (SMCs), E2, equally stimulated reendothelialization. By contrast, only E2 accelerated...
Objective: ERα (estrogen receptor alpha) exerts nuclear genomic actions and also rapid membrane-initiated steroid signaling. The mutation of the cysteine 451 into alanine in vivo has recently revealed key role this palmitoylation site on some vasculoprotective 17β-estradiol (E2) fertility. Here, we studied arginine 260 which been described to be involved its E2-induced signaling with PI-3K (phosphoinositide 3-kinase) as well G protein cultured cell lines. Approach Results: We generated a...
Background Estrogen Receptor α (ERα) is a significant modulator of energy balance and lipid/glucose metabolisms. Beyond the classical nuclear actions receptor, rapid activation intracellular signaling pathways mediated by sub-fraction ERα localized to plasma membrane, known as Membrane Initiated Steroid Signaling (MISS). However, whether membrane involved in protective metabolic endogenous estrogens conditions nutritional challenge, thus contributes sex differences susceptibility diseases,...
Estrogen receptor-α (ERα) acts primarily in the nucleus as a transcription factor involving two activation functions, AF1 and AF2, but it can also induce membrane-initiated steroid signaling (MISS) through modulation of various kinase activities and/or secondary messenger levels. Previous work has demonstrated that nuclear ERα is required for protective effect estrogen 17β-estradiol (E2), whereas selective ERαMISS sufficient to confer protection cortical not cancellous bone. The aim this...
Estrogen receptor alpha (ERα) activation by estrogens prevents atheroma through its nuclear action, whereas plasma membrane-located ERα accelerates endothelial healing. The genetic deficiency of was associated with a reduction in flow-mediated dilation (FMD) one man. Here, we evaluated ex vivo the role on FMD resistance arteries. FMD, but not agonist (acetylcholine, insulin)-mediated dilation, reduced male and female mice lacking ( Esr1 -/- mice) compared to wild-type dependent presence...
Chronic nonhealing wounds are a substantial medical concern and associated with morbidity mortality; thus, new treatment strategies required. The first step toward personalized/precision medicine in this field is probably taking sex differences into account. Impaired wound healing augmented by ischemia, we previously demonstrated that 17β-estradiol exerts major preventive effect against ischemia-induced skin flap necrosis female mice. However, the equivalent effects of testosterone male mice...
17β-Estradiol induces the postnatal development of mammary gland and influences breast carcinogenesis by binding to estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through fraction expressed at membrane. Here, we have used C451A-ERα mouse model mutated for palmitoylation site understand how membrane affects development. Although overall structure physiological is slightly affected, both epithelial fragments basal cells isolated from glands failed...
Estetrol (E4), a natural estrogen synthesized by the human fetal liver, is currently evaluated in phase III clinical studies as new menopause hormone therapy. Indeed, E4 significantly improves vasomotor and genito-urinary menopausal symptoms prevents bone demineralization. Compared with other estrogens, was found to have limited effects on coagulation factors liver of women allowing expect less thrombotic events. To fully delineate its potential, aim this study assess effect metabolic...