A5050750512- Estrogen and related hormone effects
- Reproductive System and Pregnancy
- Angiogenesis and VEGF in Cancer
- Menopause: Health Impacts and Treatments
- Airway Management and Intubation Techniques
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cardiac, Anesthesia and Surgical Outcomes
- Hypothalamic control of reproductive hormones
- Pregnancy and preeclampsia studies
- Inflammatory mediators and NSAID effects
- Atherosclerosis and Cardiovascular Diseases
- Reproductive Physiology in Livestock
- Nitric Oxide and Endothelin Effects
- Antibiotic Resistance in Bacteria
- Tracheal and airway disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer Cells and Metastasis
- T-cell and B-cell Immunology
- Antioxidant Activity and Oxidative Stress
- Cardiac Arrest and Resuscitation
- Nuclear Receptors and Signaling
- Hormonal and reproductive studies
- Hormonal Regulation and Hypertension
- Ovarian function and disorders
Institut des Maladies Métaboliques et Cardiovasculaires
2016-2025
Inserm
2016-2025
Université Toulouse III - Paul Sabatier
2016-2025
Centre Hospitalier Universitaire de Toulouse
2010-2024
Université de Toulouse
2015-2024
Hôpital Rangueil
2008-2019
Institut Gustave Roussy
2002-2018
Centre National de la Recherche Scientifique
1990-2017
Université de Bordeaux
2008-2017
École Nationale Vétérinaire de Toulouse
2012-2017
Abstract The nonpolymorphic soluble HLA-G1 (sHLA-G1) isoform has been reported to be secreted by trophoblast cells at the materno-fetal interface, suggesting that it may act as immunomodulator during pregnancy. In this paper, we report affinity-purified β2-microglobulin-associated sHLA-G1 triggered apoptosis in activated, but not resting CD8+ peripheral blood cells. We demonstrate Western blotting enhanced CD95 ligand expression activated Cytotoxicity was inhibited preincubation of with a...
Significance The in vivo roles of plasma membrane-associated estrogen receptor (ER)α, including cross-talk with nuclear ERα, are poorly understood. We created a mouse point mutation the palmitoylation site ERα (C451A-ERα) to obtain membrane-specific loss function. A complementary lacking activation function AF-2 (ERα-AF2 0 ) provided selective actions. Physiologic studies revealed critical requirements for membrane receptors ovarian and thereby fertility, vascular physiology. In contrast,...
To provide an update to French guidelines about "Difficult intubation and extubation in adult anaesthesia 2006".A consensus committee of 13 experts was convened. A formal conflict-of-interest (COI) policy developed at the onset process enforced throughout. The entire conducted independent any industry funding. authors were advised follow principles Grading Recommendations Assessment, Development Evaluation (GRADE) system guide assessment quality evidence. potential drawbacks making strong...
Using four different HLA-G-recognizing monoclonal antibodies (mAb), we investigated whether this nonclassical HLA class I molecule could be expressed in placental cell types other than extravillous cytotrophoblasts (evct) which HLA-G has already been detected. Immunohistochemical analysis was performed on serial cryosections of first trimester placenta as well maternal decidual tissue. In addition to some proliferative evct, the recently described BFL.1 mAb also slightly stained villous...
Circulating human natural killer (NK) lymphocytes have been functionally defined by their ability to exert cytotoxic activity against MHC class I-negative target cell lines, including K562. Therefore, it was proposed that NK cells recognized the “missing self.” We show here Ig-like CD160 receptor expressed circulating CD56 dim+ or IL-2-deprived lines is mainly involved in K562 cells. Further, we report HLA-C molecules are constitutively trigger lysis through engagement. In addition,...
17β-Estradiol (E2) regulates estrogen receptor-α (ERα) target gene transcription through the two independent activation functions (AFs), AF1 and AF2, located in N-terminal ligand binding domain of ERα, respectively. We previously reported that ERα is required for E2 atheroprotective action as well its accelerative on endothelial healing, but function dispensable. Here, we investigated role ERαAF2 these major beneficial actions by electively targeting (named 0 ) . Our results prove four...
Full-length 66-kDa estrogen receptor alpha (ERalpha) stimulates target gene transcription through two activation functions (AFs), AF-1 in the N-terminal domain and AF-2 ligand binding domain. Another physiologically expressed 46-kDa ERalpha isoform lacks A/B domains is consequently devoid of AF-1. Previous studies cultured endothelial cells showed that might not be required for estradiol (E2)-elicited NO production. To evaluate involvement vasculoprotective actions E2, we generated a...
Background— The prevention of early atheroma by estrogens has been clearly demonstrated in all animal models and appears to be mediated through a direct action on the arterial wall rather than an effect lipoprotein profile. goal present study was evaluate which cellular target is crucial this beneficial estradiol. Methods Results— We first confirmed key role estrogen receptor-α (ERα) atheroprotective estradiol, because completely abolished mice deficient both low-density receptor (LDLr) ERα....
Estrogens directly promote the growth of breast cancers that express estrogen receptor α (ERα). However, contribution stromal expression ERα in tumor microenvironment to protumoral effects has never been explored. In this study, we evaluated molecular and cellular mechanisms by which 17β-estradiol (E2) impacts modulates development ERα-negative tumors. Using different mouse models ER-negative cancer cells grafted subcutaneously into syngeneic ovariectomized immunocompetent mice, found E2...
Objective We evaluated the influence of sex on pathophysiology non-alcoholic fatty liver disease (NAFLD). investigated diet-induced phenotypic responses to define sex-specific regulation between healthy and NAFLD identify influential pathways in different preclinical murine models their relevance humans. Design Different (high-fat diet, choline-deficient high-fat Western diet or supplemented with fructose glucose drinking water) were compared a control male female mice. performed metabolic...
One major materno-fetal interface in the human placenta is constituted by syncytiotrophoblast, contact with maternal blood of intervillous space, which derives from differentiation and fusion villous cytotrophoblast (vct). In present work, we purified vct term depleting HLA class I- II-positive cells. We found RT-PCR that both soluble intron 4-retaining HLA-G1 (sHLA-G1) HLA-G2 isoforms were transcribed vct. Using different HLA-G-specific mAb, demonstrated intracellular flow cytometry,...
The human MHC class Ib antigen HLA-G is thought to regulate maternal immune responses during pregnancy. Here we show that expression of in transgenic mice diminished cellular immunity by inhibiting maturation myelomonocytic cells into functional antigen-presenting (APC). Skin allografts applied survived longer and resultant T cell were less potent compared control mice. from responded normally allogeneic APC immunohistological analyses spleen revealed no marked abnormalities. However,...
Fibroblast growth factor 2 (FGF2) is a major angiogenic involved in angiogenesis and arteriogenesis, however the regulation of its expression during these processes poorly documented. FGF2 mRNA contains an internal ribosome entry site (IRES), translational regulator expected to allow cellular stress.In present study, we have developed skin ischemia model transgenic mice expressing reporter transgene under control IRES. The results reveal that induced at protein level ischemia, concomitant...