A5091890488- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Down syndrome and intellectual disability research
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Mesenchymal stem cell research
- Vitamin D Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Circular RNAs in diseases
- Genetics, Bioinformatics, and Biomedical Research
- Autism Spectrum Disorder Research
- RNA Interference and Gene Delivery
- Congenital heart defects research
- Computational Drug Discovery Methods
- PI3K/AKT/mTOR signaling in cancer
- Virus-based gene therapy research
- Iron Metabolism and Disorders
- Nerve injury and regeneration
- Nutrition, Genetics, and Disease
- Treatment of Major Depression
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Coccidia and coccidiosis research
Universiti Putra Malaysia
2016-2025
McMaster University
2023-2025
University of Toronto
2024
Impact
2024
Queens University
2024
Harvard University
2019-2023
Simon Fraser University
2022
Jaffna Teaching Hospital
2020
Serdang Hospital
2014-2019
Northeastern University
2018
The molecular basis of the earliest neuronal changes that lead to Alzheimer's disease (AD) is unclear. Here, we analyze neural cells derived from sporadic AD (SAD), APOE4 gene-edited and control induced pluripotent stem (iPSCs). We observe major differences in iPSC-derived progenitor (NP) neurons gene networks related differentiation, neurogenesis, synaptic transmission. SAD patients exhibit accelerated differentiation reduced cell renewal. Moreover, a similar phenotype appears NP cerebral...
Impulse control disorder (ICD) and behaviours (ICB) represent a group of behavioural disorders that have become increasingly recognised in Parkinson's disease (PD) patients who previously used dopaminergic medications, particularly dopamine agonists levodopa. It has been suggested these medications can lead to the development ICB through abnormal modulation transmission signalling mesocorticolimbic system. Several studies reported an association between polymorphisms receptor (DRD)...
We investigated the effects of zerumbone (1 and 10 mg/kg) against hyperactivity, anxiety memory impairment in scopolamine-induced dementia Sprague-Dawley rats.Open field tests, elevated plus maze Morris water were performed to assess general locomotor activity, anxiety-like behaviours learning processes respectively rats pretreated with scopolamine.Scopolamine-treated showed high total stereotype, distance travelled open arena, reduced number entries arms, decreased percentage time spent...
Abstract We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free method spatially-resolved, targeted, in situ identification of single or multiple targets. BOLORAMIS was demonstrated range cell types human cerebral organoids. Singleplex experiments to detect coding non-coding RNAs iPSCs showed stem-cell signature pattern. Specificity found be 92% as illustrated by clear distinction between mouse...
The Ts1Cje mouse model of Down syndrome (DS) has partial triplication chromosome 16 (MMU16), which is partially homologous to human 21. These mice develop various neuropathological features identified in DS individuals. We analysed the effect MMU16 segment on global gene expression cerebral cortex, cerebellum and hippocampus at 4 time-points: postnatal day (P)1, P15, P30 P84. Gene profiling a total 317 differentially expressed genes (DEGs), selected from spatiotemporal comparisons, between...
MicroRNAs (miRNAs) are small non-coding RNAs that can exert multilevel inhibition/repression at a post-transcriptional or protein synthesis level during disease development. Characterisation of miRNAs in adult mammalian brains by deep sequencing has been reported previously. However, to date, no RNA profiling the developing brain undertaken using this method. We have performed and analysis (E15.5) mouse brain.We identified expression 294 known E15.5 brain, which were mostly represented let-7...
Background Brain-derived neurotrophic factor (BDNF) is a neurotrophin found in abundance brain regions such as the hippocampus, cortex, cerebellum and basal forebrain. It has been associated with risk of susceptibility to major depressive disorder (MDD). This study aimed determine association three BDNF variants (rs6265, rs1048218 rs1048220) Malaysian MDD patients. Methods The correlation these plasma level among patients was assessed. A total 300 cases matched controls recruited from four...
Polycystic ovary syndrome (PCOS) affects reproductive-age women. This condition causes infertility, insulin resistance, obesity, and heart difficulties. The molecular basis mechanism of PCOS might potentially generate effective treatments. Long non-coding RNAs (lncRNAs) show control over multifactorial disorders' growth incidence. Numerous studies have emphasized its significance alterations in PCOS. We used bioinformatic methods to find novel dysregulated lncRNAs To achieve this objective,...
Bipolar disorder (BD) is one of the major psychiatric diseases in which impairment mitochondrial functions has been closely connected or associated with disease pathologies. Different lines evidence close connection between mitochondria dysfunction and BD were discussed a particular focus on (1) dysregulation energy metabolism, (2) effect genetic variants, (3) oxidative stress, cell death apoptosis, (4) dysregulated calcium homeostasis electrophysiology, (5) current as well potential...
Increasing shreds of evidence suggest that neurogenic-to-gliogenic shift may be critical to the abnormal neurodevelopment observed in individuals with Down syndrome (DS). REST, Repressor Element-1 Silencing Transcription factor, regulates differentiation and development neural cells. Downregulation REST lead defects post-differentiation neuronal morphology brain DS fetal. This study aims elucidate role DS-derived NPCs using bioinformatics analyses laboratory validations. We identified...
Ruxolitinib is a Janus kinase (JAK) inhibitor that inhibits the JAK/STAT signalling pathway by targeting JAK1 and JAK2, which are crucial for regulating astrogliogenesis. This study assessed effect of ruxolitinib (5 30 mg/kg/day) on developing mouse brains administering it to pregnant mice from E7.5 E20.5. No adverse effects were observed in treated mice. The P1.5 pups collected, RNA was extracted assess markers neurogenesis astrogliogenesis through RT-qPCR. results revealed significant...
Up to 15% of survivors COVID-19 infection experience long-term health effects, including fatigue, myalgia and impaired cognitive function, termed post-COVID-19 condition or long COVID. Several trials that study the benefits harms various interventions manage COVID have been published hundreds more are planned ongoing. Trustworthy systematic reviews clarify critical promote evidence-based practice. To create maintain a living review network meta-analysis addressing pharmacologic...
Poor reproducibility and high inter-individual variability in responses to intermittent theta burst stimulation (iTBS) of the human motor cortex (M1) are matters concern. Here we recruited 17 healthy young adults a randomized, sham-controlled, crossover study. Transcranial magnetic (TMS)-elicited evoked potentials (MEPs) were measured pre-iTBS (T0) post-iTBS at 4-7 (T1), 9-12 (T2), 17-20 (T3), 27-30 minutes (T4) from right first dorsal interosseous muscle. MEP grand average (MEPGA) was...
Introduction Accumulating evidence indicates that the increased presence of astrocytes is fundamentally linked to neurological dysfunctions observed in individuals with Down syndrome (DS). REST (RE1-silencing transcription factor), as a chromatin modifier, regulates 15,450 genes humans. key regulatory element governs astrocyte differentiation, development, and maintenance their physiological functions. The downregulation may disrupt homeostatic balance DS. Methods This study aims elucidate...