Studies have discovered that different extracts of Evodia rutaecarpa and its phytochemicals show a variety biological activities associated with inflammation. Although rutaecarpine, an alkaloid isolated from the unripe fruit E. rutaecarpa, has been exposed to anti-inflammatory properties, mechanism action not well studied. Thus, this study investigated molecular mechanisms rutaecarpine (RUT) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. RUT reserved production nitric oxide (NO)...

Platelets assume a pivotal role in the cardiovascular diseases (CVDs). Thus, targeting platelet activation is imperative for mitigating CVDs. Ginkgetin (GK), from Ginkgo biloba L, renowned its anticancer and neuroprotective properties, remains unexplored concerning impact on activation, particularly humans. In this investigation, we delved into intricate mechanisms through which GK influences human platelets. At low concentrations (0.5-1 μM), exhibited robust inhibition of collagen...

Inflammation, a fundamental response to infection and injury, involves interactions among immune cells signaling molecules. Dysregulated inflammation contributes diseases such as autoimmune disorders cancer. Interleukin-1 beta (IL-1β), produced by macrophages in lipoteichoic acid (LTA) from Gram-positive bacteria, is key inflammatory mediator. Glabridin (GBD), bioactive compound licorice root, exhibits anti-inflammatory properties. This study investigates GBD’s effects on LTA-induced...

Inflammation is a major root of several diseases such as allergy, cancer, Alzheimer’s, and others, the present state existing drugs provoked researchers to search for new treatment strategies. Plants are regarded be unique sources active compounds holding pharmacological properties, they offer novel designs in development therapeutic agents. Therefore, this study aimed explore anti-inflammatory mechanism esculetin lipoteichoic acid (LTA)-induced macrophage cells (RAW 264.7). The relative...

Esculetin, a bioactive 6,7-dihydroxy derivative of coumarin, possesses pharmacological activities against obesity, diabetes, renal failure, and cardiovascular disorders (CVDs). Platelet activation plays major role in CVDs. Thus, disrupting platelet represents an attractive therapeutic target. We examined the effect esculetin human experimental mouse models. At 10–80 μM, inhibited collagen- arachidonic acid-induced aggregation washed platelets. However, it had no effects on other agonists...

Lipoteichoic acid (LTA) is a key cell wall component and virulence factor of Gram-positive bacteria. LTA contributes major role in infection it mediates inflammatory responses the host. Rutaecarpine, an indolopyridoquinazolinone alkaloid isolated from Evodia rutaecarpa, has shown variety fascinating biological properties such as anti-thrombotic, anticancer, anti-obesity thermoregulatory, vasorelaxing activity. It also potent effects on cardiovascular endocrine systems. Herein, we...

Platelets assume a pivotal role in the pathogenesis of cardiovascular diseases (CVDs), emphasizing their significance disease progression. Consequently, addressing CVDs necessitates targeted approach focused on mitigating platelet activation. Eugenol, predominantly derived from clove oil, is recognized for its antibacterial, anticancer, and anti-inflammatory properties, rendering it valuable medicinal agent. This investigation delves into intricate mechanisms through which eugenol influences...

Platelets are crucial for hemostasis and arterial thrombosis, which may lead to severe cardiovascular diseases (CVDs). Thus, therapeutic agents must be developed prevent pathological platelet activation. Glabridin, a major bioalkaloid extracted from licorice root, improves metabolic abnormalities (i.e., obesity diabetes) protects against CVDs neuronal disorders. To the best of our knowledge, no studies have focused on glabridin’s effects Therefore, we investigated these in humans mice....

Objective. Oxidative stress-mediated inflammatory events involve in the progress of several diseases such as asthma, cancers, and multiple sclerosis. Auraptene (AU), a natural prenyloxycoumarin, possesses numerous pharmacological activities. Here, anti-inflammatory effects AU were investigated lipoteichoic acid- (LTA-) induced macrophage cells (RAW 264.7). Methods. The expression cyclooxygenase (COX-2), tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), inducible nitric oxide synthase...

Platelets play a crucial role in the physiology of primary hemostasis and pathological processes such as arterial thrombosis; thus, developing therapeutic target that prevents platelet activation can reduce thrombosis. Pterostilbene (PTE) has remarkable pharmacological activities, including anticancer neuroprotection. Few studies have reported effects pterostilbene on activation. Thus, we examined inhibitory mechanisms human platelets its vascular thrombosis prevention mice. At low...

Platelets play a vital role in the formation of dangerous arterial thrombosis. are activated by adhesive proteins or soluble agonists through their specific receptors. The receptor-mediated signaling pathways lead to common events, which result shape changes and inside-out signaling, leading fibrinogen binding glycoprotein IIb/IIIa complex (integrin αIIbβ3). This interaction initiates integrin αIIbβ3-mediated outside-in subsequently culminating granule secretion aggregation. Myricetin is...

Auraptene is the most abundant coumarin derivative from plants. The pharmacological value of this compound has been well demonstrated, especially in prevention cancer and neurodegenerative diseases. Platelet activation a major factor contributing to arterial thrombosis. Thus, study evaluated influence auraptene platelet aggregation thrombotic formation. inhibited human platelets stimulated with collagen only. However, was not effective inhibiting thrombin, arachidonic acid, U46619. also...

Pterostilbene (PTE), a natural stilbenoid occurring in grapes and berries, is recognized as dimethylated analogue of resveratrol. This compound shows numerous notable pharmacological activities, including antiaging, anticancer, antidiabetes, antioxidant, neuroprotection. study investigates the anti-inflammatory properties PTE macrophage cells (RAW 264.7) against lipoteichoic acid (LTA) stimulation. The expression inflammatory tumor necrosis factor (TNF-α), interleukin-1β (IL-1 β), inducible...

Morin hydrate, a bioactive flavonoid, has been proven to prevent inflammation and apoptosis of cells. Flavonoids can reduce the risk cardiovascular diseases, in which platelet activation plays major role. This study investigated effect morin hydrate on vitro vivo. markedly inhibited aggregation stimulated by collagen human platelets but not that other agonists. In collagen-activated platelets, adenosine triphosphate (ATP) release; intracellular Ca2+ mobilization; P-selectin expression;...

Several studies have reported that metal complexes exhibit anti-inflammatory activities; however, the molecular mechanism is not well understood. In this study, we used a potent ruthenium (II)-derived compound, [Ru(η6-cymene)2-(1H-benzoimidazol-2-yl)-quinoline Cl]BF4 (TQ-6), to investigate mechanisms underlying effects against lipopolysaccharide (LPS)-induced macrophage activation and liver injury in mice. Treating LPS-stimulated RAW 264.7 cells with TQ-6 suppressed nitric oxide (NO)...

Activated microglia are crucial in the regulation of neuronal homeostasis and neuroinflammation. They also contribute to neuropathological processes after ischemic stroke. Thus, finding new approaches for reducing neuroinflammation has gained considerable attention. The metal ruthenium notable attention because its ability form complexes that can be used disease treatment. [Ru(η6-cymene)2-(1H-benzoimidazol-2-yl)-quinoline Cl]BF4 (TQ-6), a potent (II)-derived compound, was this study...

Objectives Oxidative stress induces chronic inflammatory diseases in aerobic organisms, and antioxidants from plants represent an efficient strategy to prevent this condition. Morin hydrate (MH), a bioactive flavonoid, has wide range of pharmacological properties, including anti-inflammatory anti-oxidant. This study evaluated the protective effects MH on lipoteichoic acid (LTA)-induced inflammation RAW 264.7 macrophages by testing main oxidative biomarkers also investigating molecular...

Abstract During platelet activation, fibrinogen binds to its specific receptor, integrin α II b β 3 , thus completing the final common pathway for aggregation. Norcantharidin ( NCTD ) is a promising anticancer agent in China from medicinal insect blister beetle. In this study, we provided evidence demonstrate (0.1–1.0 μM) possesses very powerful antiplatelet activity human platelets; nevertheless, it had no effects on surface P‐selectin expression and only slight inhibition ATP ‐release...

Myocardial infarction and cerebral ischemic stroke are prominent causes of death worldwide. Platelets play major roles in these diseases, although they anucleated cells, but also express the NF-κB. Pterostilbene (PTE) possesses some intriguing pharmacological properties, including capacity to inhibit platelet activation. We investigated inhibitory role PTE NF-κB-mediated signal events compared relative potency with that classical NF-κB inhibitors. IκB kinase (IKK) inhibitor, BAY11-7082,...

Platelets play a critical role in arterial thrombosis. Rutaecarpine (RUT) was purified from

Platelet activation plays a major role in cardio and cerebrovascular diseases, cancer progression. Disruption of platelet represents an attractive therapeutic target for reducing the bidirectional cross talk between platelets tumor cells. Platinum (Pt) compounds have been used treating cancer. Hence, replacing Pt with iridium (Ir) is considered potential alternative. We recently developed Ir(III)-derived complex, [Ir(Cp*)1-(2-pyridyl)-3-(2-hydroxyphenyl)imidazo[1,5-a]pyridine Cl]BF4 (Ir-11),...

Platelets play a major role in hemostatic events and are associated with various pathological events, such as arterial thrombosis atherosclerosis. Iridium (Ir) compounds potential alternatives to platinum compounds, since they exert promising anticancer effects without cellular toxicity. Our recent studies found that Ir show potent antiplatelet properties. In this study, we evaluated the vitro antiplatelet, vivo antithrombotic structure⁻activity relationship (SAR) of newly synthesized...

Macrophages are essential immune cells involved in maintaining physiological homeostasis and mediating pathological responses. Activation of macrophages by lipoteichoic acid (LTA) from Gram-positive bacteria triggers inflammatory pathways, leading to the production pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) inducible nitric oxide synthase (iNOS). Understanding regulation these pathways is crucial for developing therapeutic strategies diseases. Our previous study has...

Objectives: Inflammation, a crucial biological response defending the body against harmful stimuli, has been central focus in medicinal research. Licorice root, rich bioactive compounds, contains glabridin (GBD), renowned natural flavonoid with anti-inflammatory and antioxidant properties. Despite its recognition, precise molecular mechanisms underlying GBD's effects remain subject of ongoing investigation. Methods: This study explores impact GBD on lipopolysaccharide (LPS)-induced...