A5011838429- Retinopathy of Prematurity Studies
- Neonatal and fetal brain pathology
- RNA modifications and cancer
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Anesthesia and Neurotoxicity Research
- Retinal Development and Disorders
- Tryptophan and brain disorders
- Neonatal Respiratory Health Research
- Stress Responses and Cortisol
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroendocrine regulation and behavior
- Retinal and Optic Conditions
- MicroRNA in disease regulation
- Muscle metabolism and nutrition
- Biochemical Analysis and Sensing Techniques
- Histone Deacetylase Inhibitors Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Analytical Chemistry and Sensors
- Neonatal Health and Biochemistry
- Mesenchymal stem cell research
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Neurotransmitter Receptor Influence on Behavior
- RNA regulation and disease
University of Massachusetts Chan Medical School
2020-2024
Montreal Children's Hospital
2013-2018
McGill University
2011-2018
McGill University Health Centre
2017-2018
Chungnam National University
2018
Seoul National University
2017-2018
Douglas Mental Health University Institute
2011-2013
Significance Fragile X syndrome (FXS), the most prevalent monogenic form of autism, is caused by loss FMRP, an RNA binding protein. In absence translation dysregulated, but restoration translational homeostasis rescues and thus could suggest new treatments for disorder. Using ribosome profiling metabolic profiling, we show that, in FMRP-deficient mouse brain, there are few specific disturbances. Instead, widespread imbalance levels. This destabilization FMRP targets other mRNAs based on...
Using inbred strains of mice that differ widely in their innate preference for and consumption ethanol, we demonstrate, ethanol-preferring C57BL/6J (C57) mice, decreased dopamine (DA) content turnover the terminals mesolimbic mesostriatal neurons, compared with ethanol-avoiding DBA/2J BALBc mice. These data suggest genetically determined hypodopaminergic function these pathways plays a role predisposition to high voluntary intake ethanol. DA areas was selectively increased by ethanol C57...
Purpose: We investigated the effects of term neonatal encephalopathy on retinal function and structure. Methods: A rat model hypoxic-ischemic (HI) (Vannucci model) was used. Hypoxia-ischemia induced by a left common carotid ligation followed 2-hour period hypoxia (8% oxygen) in Long-Evans pups at postnatal day 10 (P10). Sham operated rats served as controls.. Retinal assessed P30 P60 electroretinograms (ERGs), after which histology performed. Retinocortical with visual evoked potentials...
ABSTRACT Many premature newborns develop bronchopulmonary dysplasia (BPD), a chronic lung disease resulting from prolonged mechanical ventilation and hyperoxia. BPD survivors typically suffer long-term injuries not only to the lungs, but also brain retina. However, currently it is clear whether retinal in these are related their prematurity, or BPD. We investigated hyperoxia known cause histologic changes lungs similar an animal model causes injuries. Sprague Dawley rat pups were exposed...
Poly(A) tail length is regulated in both the nucleus and cytoplasm. One factor that controls polyadenylation cytoplasm CPEB1, an RNA binding protein associates with specific mRNA 3′UTR sequences to tether enzymes add remove poly(A). Two of these enzymes, noncanonical poly(A) polymerases GLD2 (TENT2, PAPD4, Wispy) GLD4 (TENT4B, PAPD5, TRF4, TUT3), interact CPEB1 extend To identify additional proteins might anchor RNA, we expressed double tagged U87MG cells, which was used for sequential...
Purpose: The purpose of this study was to investigate the effects sildenafil on retinal injury following neonatal hypoxia-ischemia (HI) at term-equivalent age in rat pups. Methods: Hypoxia-ischemia induced male Long-Evans pups postnatal day 10 (P10) by a left common carotid ligation followed 2-hour exposure 8% oxygen. Sham-operated rats served as control group. Both groups were administered vehicle or 2, 10, 50 mg/kg sildenafil, twice daily for 7 consecutive days. Retinal function assessed...
Fragile X syndrome (FXS) is a neurodevelopmental disorder that often modeled in Fmr1 knockout mice where the RNA-binding protein FMRP absent. Here, we show -deficient mice, RNA mis-splicing occurs several brain regions and peripheral tissues. To assess molecular mechanisms of splicing mis-regulation, employed N2A cells depleted . In absence FMRP, RNA-specific exon skipping events are linked to factors hnRNPF, PTBP1, MBNL1. regulates translation Mbnl1 mRNA as well auto-splicing. Elevated...
Microglia are myeloid cells of the central nervous system that perform tasks essential for brain development, neural circuit homeostasis, and disease. react to inflammatory stimuli by upregulating signaling through several different immune cell receptors such as Toll-like receptor 4 (TLR4), which signals downstream effectors including transforming growth factor beta-activated kinase 1 (TAK1). Here, we show TAK1 levels regulated CPEB1, a sequence-specific RNA binding protein controls...
Background: Oxygen therapy provided to support the lungs of premature newborns often leads damages retina called retinopathy prematurity (ROP) and long-term visual impairments. Current treatments for ROP are invasive aim at preventing further progression retina, but do not repair these damages. Our goal is investigate therapeutic effect sildenafil on retinal structure in a rat model ROP. Methods: Sprague-Dawley rats were exposed hyperoxia (i.e., 80% oxygen) interrupted by three 0.5-hour...
Background: Retinal pigment epithelium (RPE) is vital for the homeostasis of subretina including photoreceptors and choroid. Interestingly, our previous results suggested that recently discovered lactate receptor GPR81 abundantly expressed in RPE. To date, only one study has shown activating could enhance DNA repair by HDAC1. Consequently, we investigated whether exhibits epigenetic modification using GPR81−/− mice. Methods: mice wide type littermates were generated on a background C57BL/6J...
Abstract Fragile X Syndrome (FXS) is a neurodevelopmental disorder that often modeled in Fmr1 knockout mice where the RNA binding protein FMRP absent. Here we show -deficient mice, mis-splicing occurs several brain regions and peripheral tissues. To assess molecular mechanisms of splicing mis-regulation, employed N2A cells depleted . In absence FMRP, RNA-specific exon skipping events are linked to factors hnRNPF, PTBP1, MBNL1. regulates translation Mbnl1 mRNA as well auto-splicing. Elevated...