A5074407518- Gastrointestinal motility and disorders
- Regulation of Appetite and Obesity
- Helicobacter pylori-related gastroenterology studies
- Neuroendocrine Tumor Research Advances
- Congenital gastrointestinal and neural anomalies
- Gastrointestinal disorders and treatments
- Diabetes Treatment and Management
- Biochemical Analysis and Sensing Techniques
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Diet and metabolism studies
- Neuroscience of respiration and sleep
- Celiac Disease Research and Management
- Receptor Mechanisms and Signaling
- Retinal Diseases and Treatments
- Microbial Metabolites in Food Biotechnology
- Effects of Environmental Stressors on Livestock
- Ion Channels and Receptors
- Microfluidic and Bio-sensing Technologies
- Dietary Effects on Health
- Digestive system and related health
- Nitric Oxide and Endothelin Effects
- Retinal Development and Disorders
- Animal Behavior and Welfare Studies
- Nutrition, Genetics, and Disease
Florey Institute of Neuroscience and Mental Health
2017-2025
The University of Melbourne
2016-2025
Walter and Eliza Hall Institute of Medical Research
2024
Ecosystem Sciences
2024
Sun Pharma Advanced Research (India)
2021
Mayo Clinic
2021
WinnMed
2021
Carl Zeiss (Australia)
2021
Monash University
2020
What is the central question of this study? Oxidative stress may play a role in compromising intestinal epithelial barrier integrity pigs subjected to heat stress, but it unknown whether an increase dietary antioxidants (selenium and vitamin E) could alleviate gut leakiness heat-stressed pigs. main finding its importance? Levels selenium (1.0 p.p.m.) E (200 IU kg(-1) ) greater than those usually recommended for reduced caused by stress. This suggests that oxidative plays also provides...
Recent studies reveal complex patterns of hormone coexpression within enteroendocrine cells (EECs), contrary to the traditional view that gut hormones are expressed individually in EECs. Moreover, different have been found separate subcellular vesicles. However, detailed analysis relative expression multiple has not made. Subcellular confined peptide hormones, and included indolamine 5-hydroxytryptamine (5-HT) or neuroendocrine protein chromogranin A (CgA). In present work, 5-HT, CgA,...
Dynamic interactions between gut mucosal cells and the external environment are essential to maintain homeostasis. Enterochromaffin (EC) transduce both chemical mechanical signals produce 5-hydroxytryptamine (5-HT) mediate disparate physiological responses. However, molecular cellular basis for functional diversity of ECs remains be adequately defined. Here, we integrated single-cell transcriptomics with spatial image analysis identify fourteen EC clusters that topographically organized...
Dynamic interactions between gut mucosal cells and the external environment are essential to maintain homeostasis. Enterochromaffin (EC) transduce both chemical mechanical signals produce 5-hydroxytryptamine mediate disparate physiological responses. However, molecular cellular basis for functional diversity of ECs remains be adequately defined. Here, we integrated single-cell transcriptomics with spatial image analysis identify 14 EC clusters that topographically organized along gut....
TRPA1 is a ligand-activated cation channel found in the intestine and other tissues. Components of food that stimulate receptors (phytonutrients) include allyl isothiocyanate, cinnamaldehyde linalool, but these may also act at receptors. Cells lining intestinal mucosa are immunoreactive for Trpa1 mRNA occurs mucosal extracts, suggesting receptor target agonists. However, situ hybridisation reveals expression 5-HT containing enteroendocrine cells, not enterocytes. agonists evoke secretion,...
Progeny born to primiparous sows (gilt progeny; GP) have lower birth, weaning and slaughter weights than sow progeny (SP). GP also reduced gastrointestinal tract (GIT) development, as evidenced by organ weights. Therefore, the aim of this experiment was quantify changes in GIT barrier function that occur birth weaning, representing two major challenges young piglet. The effects parity (GP vs. SP) integrity were quantified at four timepoints: (~0 h), 24 h after (24 1-d preweaning (PrW),...
Purpose: Vision loss caused by photoreceptor death represents one of the first symptoms in neuronal ceroid lipofuscinosis, a condition characterized accumulation intracellular waste. Cln6nclf mice have naturally occurring mutation ceroid–lipofuscinosis (CLN) protein 6 and are model this disorder. In order to identify effect waste (lipofuscin) plays driving retinal degeneration, time course degeneration was carefully functionally using electroretinogram structurally histology. Methods:...
We investigated the distributions and targets of nitrergic neurons in rat stomach, using neuronal nitric oxide synthase (NOS) immunohistochemistry nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry. Nitrergic comprised similar proportions myenteric neurons, about 30%, all gastric regions. Small numbers occurred submucosal ganglia. In total, there were ~ 125,000 (nNOS) stomach. The cell bodies had single axons, type I morphology a wide range sizes. Five identified,...
Dynamic interactions between gut mucosal cells and the external environment are essential to maintain homeostasis. Enterochromaffin (EC) transduce both chemical mechanical signals produce 5-hydroxytryptamine (5-HT) mediate disparate physiological responses. However, molecular cellular basis for functional diversity of ECs remains be adequately defined. Here, we integrated single-cell transcriptomics with spatial image analysis identify fourteen EC clusters that topographically organized...
Abstract Background Dopamine receptor 2 (DRD2) and ghrelin (GHSR1a) agonists both stimulate defecation by actions at the lumbosacral center. is in nerve terminals surrounding autonomic neurons of center, whereas not present spinal cord. D2 receptors generally inhibits neurons, but its effect excitatory. Methods In vivo recording colorectal propulsion was used to investigate interaction between DRD2 GHSR1a. Localization studies were determine sites expression rat human Key Results Dopamine,...
ABSTRACT Patients with Hirschsprung disease lack enteric ganglia in the distal colon and propulsion of colorectal content is substantially impaired. Proposed stem cell therapies to replace neurons require surgical bypass aganglionic bowel during re-colonization, but there inadequate knowledge consequences bypass. We performed surgery Ednrb−/− rat pups. Surgically rescued rats failed thrive, an outcome reversed by supplying electrolyte- glucose-enriched drinking water. Histologically,...