A5060665937- Fungal and yeast genetics research
- Bacterial Genetics and Biotechnology
- Gene expression and cancer classification
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Skin Protection and Aging
- Telomeres, Telomerase, and Senescence
- Genomic variations and chromosomal abnormalities
- Allergic Rhinitis and Sensitization
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Organ Transplantation Techniques and Outcomes
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Cytokine Signaling Pathways and Interactions
- Plant Virus Research Studies
- Reproductive System and Pregnancy
- Melanoma and MAPK Pathways
- Food Allergy and Anaphylaxis Research
- Dermatology and Skin Diseases
- Bone fractures and treatments
- Extracellular vesicles in disease
- Congenital limb and hand anomalies
Yonsei University
2005-2017
Ewha Womans University
2016
Yunnan Agricultural University
2010
Army Medical University
2010
Southwest Hospital
2010
Catholic University of Korea
2009
Boston University
2005
The University of Texas Southwestern Medical Center
1999-2001
Howard Hughes Medical Institute
1999
Rockefeller University
1999
Georgia A. Patikoglou, Joseph L. Kim, Liping Sun, Sang-Hwa Yang, Thomas Kodadek, and Stephen K. Burley Laboratories of Molecular Biophysics, Howard Hughes Medical Institute, The Rockefeller University, New York, York 10021 USA; Departments Internal Medicine Biochemistry, Ryburn Cardiology Center, University Texas Southwestern Dallas, 75235-8573 USA
The microbial environment is an important factor that contributes to the pathogenesis of atopic dermatitis (AD). Recently, it was revealed not only bacteria itself but also extracellular vesicles (EVs) secreted from affect allergic inflammation process. However, almost all research carried out so far related local microorganisms, systemic distribution. We aimed compare bacterial EV composition between AD patients and healthy subjects experimentally find beneficial effect some composition.
We have identified an 8-gene signature with significant expression differences between gastric cancer and normal tissues. This set can predict the status of tissues more than 96% accuracy in a totally independent microarray dataset. The 8 genes are composed down-regulated KLF4, GPX3, SST LIPF, together up-regulated SERPINH1, THY1 INHBA cancer. To corroborate differential gene pattern, we chose GPX3 examined its pattern detail. A comparison shows broader multiple types cancers, including...
Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how regulates cancer unclear. In human adenocarcinoma tissue samples, we found that both mRNA and protein of were increased, compared with neighboring normal epithelium, suggesting plays functional roles cells. Consistent this notion, syndecan-2-overexpressing HT-29 cells showed enhanced migration/invasion, anchorage-independent growth, primary tumor formation nude mice,...
The Ni(II) complex of the tripeptide NH2-Gly-Gly-His-COOH is shown to mediate efficient protein-protein cross-linking in presence oxidants such as oxone and monoperoxyphthalic acid. Only proteins that associate specifically solution are cross-linked under these conditions. Preliminary probes mechanism reaction suggest active intermediate may be a high-valent metal attacks aromatic amino acids.
Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a natural compound found in large quantities, most notably grapes and red wine, which has been shown to have anti-inflammatory, chemopreventive anti-angiogenic effects. We examined whether resveratrol any effect on growth gene expression the human ovarian cancer PA-1 cells. show that inhibits cell induces apoptosis also investigated of changes global during resveratrol-induced inhibition cells using cDNA microarray with 7,448...
The mediator is an approximately 20 protein complex that essential for the transcription of most genes in yeast. It contacted by a number gene-specific activators, but details these interactions are not well understood cases. Here, evidence presented component Gal11 represents at least one target Gal4 activation domain (AD). Deletion shown to decrease affinity AD mediator, and direct binding N-terminal with demonstrated. Quantitative studies, however, indicate KD 1:1 AD−Gal11 modest....
There has been considerable interest in virulence genes the plasticity region of Helicobacter pylori , but little is known about many these genes. JHP940, one factors encoded by H. strain J99, a proinflammatory protein that induces tumor necrosis factor-alpha and interleukin-8 secretion as well enhanced translocation NF-κB cultured macrophages. Here we have characterized structure function JHP940 to provide framework for better understanding its role inflammation . Our work demonstrates...
Atopic dermatitis (AD) is an inflammatory skin disease, significantly affecting the quality of life. Using AD as a model system, we tested successive identification AD-associated microbes, followed by culture-independent serum detection identified microbe.
Purpose: Chitin is a potent adjuvant in the development of immune response to inhaled allergens airways.According other studies, chitin known as multi-faced adjuvants which can induce Th2 responses.Recently, we found that TNF-α key mediator cell allergens.Here, evaluated immunologic mechanisms airway hypersensitivity allergens, enhanced by house dust mite (HDM)-derived chitin.Methods: The role and TLRs was an mouse model induced sensitization with allergen (ovalbumin, OVA) HDM-derived using...
Repetitive exposure to ultraviolet B (UVB) radiation is known cause DNA damage and increase levels of reactive oxy-gen species (ROS), which are linked the upregulation matrix metallopeptidases (MMPs) inflammatory cyto-kines. These events lead collagen breakdown by MMPs, resulting in wrinkles, loss elasticity, rough, dry skin—typical signs photoaging—and may even contribute skin cancers long term. We have previously shown that Solanum tuberosum-Derived Exosomes (SDE) can repress 80%...
Background/Objectives: Potato (Solanum tuberosum)-derived exosomes (SDEs) are extracellular vesicles (66 nm in diameter) with therapeutic potential. SDEs suppress matrix metallopeptidases (MMPs) 1, 2, and 9, tumor necrosis factor (TNF), interleukin 6 (IL6), while exhibiting radical-scavenging activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) vitro mitigating hydrogen peroxide (H2O2)-induced oxidative stress HaCaT cells. upregulate antioxidant gene glutathione...
Abstract Aberrant constitutive expression of the NF‐κB c‐Rel and RelA subunits in breast cancer cells was shown to promote their survival. Recently, we demonstrated that aggressive cancers constitutively express high levels RelB subunit, which promotes more invasive phenotype via induction BCL2 gene. As these are frequently resistant therapy, here tested hypothesis High expressing Hs578T MDA‐MB‐231 were γ‐radiation than MCF7 ZR‐75 cells, lower levels. Knockdown led decreased survival...
There are hump, humpless cattle and gayal distributed in Yunnan province, south-west China, but their genetic background remains unclear. To determine the origin diversity of (Diqing, Nujiang Wenshan cattle), we analysed mtDNA control region sequences 71 samples SRY gene 39 samples, together with available GenBank. The neighbour-joining phylogeny reduced median network analysis showed that originated from hybridization between male Bos frontalis female taurus or indicus, mostly B. also...
Accumulating evidence has shown that the Toll-like receptor 7 agonist imiquimod (IMQ) induces psoriasiform skin inflammation in mice and this is dependent on IL-23/IL-17 axis. Moreover, it been demonstrated main source of IL-17 not Th17 but dermal gamma delta (γδ) T cells mouse skin. Recent advances understanding immunopathogenesis psoriasis led to an alteration treatment paradigm use highly efficacious biologics. However, their high cost impedes extensive these agents. Thus, inexpensive...
This study examined the gene copy number change events at chromosome 20 in gastric cancer, and their possible relationship with recurrence using cDNA microarray-based comparative genomic hybridization.Thirty pairs of tumor normal tissues were used hybridization. The microarrays containing 17,000 sequence-verified human probes a direct comparison design, where DNAs from labeled fluorescent dyes Cy3 Cy5, respectively, cohybridized. Genes log(2) (Cy5/Cy3) > or = 0.58 least one case selected as...
ABSTRACT Fourteen population samples of Drosophila pavani were obtained from a number localities in Chile. The populations sampled dispersed over 7 degrees latitude and 1800 meters elevation, drawn at three different times. Sixteen electrophoretic loci assayed for each population; eight the analyzed statistically geographic variation; other essentially monomorphic. For all variable loci, variation allelic frequencies among was highly significant. In cases, significant portion associated with...
<b><i>Background:</i></b> There has been a paucity of data about the difference in gene expression between melasma lesional skin and normal adjacent one. <b><i>Objective:</i></b> Our aim was to identify novel genes involved pathogenesis melasma. <b><i>Methods:</i></b> We performed microarray analysis confirmed results on quantitative real-time polymerase chain reaction (qRT-PCR) Korean women with...
Although various marine ingredients have been exploitedfor the development of cosmetic products, no previous study has examined potential seaweed extracellular vesicles (EV) in such applications.Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis MNT-1 human melanoma cells, associated with downregulation MITF (microphthalmia-associated transcription factor), tyrosinase TRP1 (tyrosinase-related proteins 1).The most...
Abstract Ribozyme possesses specific endoribonuclease activity and catalyzes the hydrolysis of phosphodiester bonds, which results in cleavage target RNA sequences. Here, we evaluated ability hammerhead ribozymes targeting human telomerase (hTR) to inhibit catalytic proliferation cancer cells. Hammerhead were designed against 7 NUX sequences located open loops hTR secondary structure. We verified ribozyme specificity by vitro assay using a synthetic substrate. Subsequently, introduced...
The site-specific recombinases Flp and R from Saccharomyces cerevisiae Zygosaccharomyces rouxii, respectively, are related proteins that belong to the yeast family of recombinases. They share approximately 30% amino acid matches exhibit a common reaction mechanism appears be conserved within larger integrase Two regions proteins, designated box I II, also harbor significantly high degree homology at nucleotide sequence level. We have analyzed properties variants carrying point mutations...