A5003938069- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Protein Kinase Regulation and GTPase Signaling
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Telomeres, Telomerase, and Senescence
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- SARS-CoV-2 and COVID-19 Research
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Respiratory viral infections research
- Pancreatic function and diabetes
Korea Advanced Institute of Science and Technology
2020
Inha University
2010-2014
Incheon Medical Center
2014
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of a present pandemic, infects human lung alveolar type (hAT2) cells. Characterizing pathogenesis crucial for developing vaccines and therapeutics. However, lack models mirroring cellular physiology pathology hAT2 cells limits study. Here, we develop feeder-free, long-term, three-dimensional (3D) culture technique derived from primary tissue investigate infection response to SARS-CoV-2. By imaging-based analysis...
Background The gliotransmitters released from astrocytes are deemed to play key roles in the glial cell-neuron communication for normal function of brain. gliotransmitters, such as glutamate, ATP, D-serine, neuropeptide Y, stored vesicles and secreted following inositol 1,4,5-trisphosphate (IP3)-induced intracellular Ca2+ releases. Yet studies on identity IP3-dependent stores remain virtually unexplored. Principal Findings We have therefore studied potential existence IP3-sensitive cytoplasm...
Mitochondrial dysfunction in dopaminergic neurons of patients with idiopathic and familial Parkinson's disease (PD) is well known although the underlying mechanism not clear. We established a homogeneous population human adipose tissue-derived mesenchymal stromal cells (hAD-MSCs) from adult early-onset hereditary Parkin-defect PD as late-onset by immortalizing hTERT gene to better understand PD. The hAD-MSCs were designated "PD", "Parkin" pituitary adenomas "non-PD" short. pGRN145 plasmid...
Phosphatidylinositol (PI) kinases are key molecules that participate in the phosphoinositide signaling cytoplasm. Despite accumulating evidence supports existence and operation of independent PI system nucleus, exact location inside nucleus is not well defined. Here we show PI4-kinases IIα IIβ, which play central roles PI(4,5)P2 synthesis signaling, localized numerous small nucleoplasmic vesicles function as inositol 1,4,5-trisphosphate (Ins(1,4,5)P3)-sensitive Ca2+ stores. This accord with...
Objectives The α and β cells of pancreatic islet release important hormones in response to intracellular Ca2+ increases that result from releases through the inositol 1,4,5-trisphoshate receptor (IP3R)/Ca2+ channels. Yet no systematic studies on distribution IP3R/Ca2+ channels have been done, prompting us investigate all 3 IP3R isoforms. Methods Immunogold electron microscopy was performed determine presence relative concentrations isoforms 2 major organelles secretory granules (SGs)...
Abstract Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), which is the cause of a present global pandemic, infects human lung alveolar cells (hACs). Characterising pathogenesis crucial for developing vaccines and therapeutics. However, lack models mirroring cellular physiology pathology hACs limits study. Here, we develop feeder-free, long-term three-dimensional (3D) culture technique type (hAT2) cells, investigate infection response to SARS-CoV-2. By imaging-based analysis...